Rapid baseline-separation of all eight tocopherols and tocotrienols by reversed-phase liquid-chromatography with a solid-core pentafluorophenyl column and their sensitive quantification in plasma and liver

Grebenstein, Nadine; Frank, Jan

doi:10.1016/j.chroma.2012.04.042

Cited by 118 publications

(82 citation statements)

References 21 publications

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“…The separation of various tocols was also carried out by reverse phase Kinetex 2.6 µ PFP 100A column (150 × 4.6 mm) attached to Security Guard ULTRA Cartridges (UHPLC PFP for 4.6 mm ID column). The eluting solvent was methanol + water (85% + 15%, vol/vol) at a flow rate of 0.8 ml/min [30]. The rest of the conditions were the same as described above.…”

Section: High Performance Liquid Chromatography Analysismentioning

confidence: 99%

“…The retention time of the individual peaks of the unknown tocols were compared against the retention time of the pure standard tocols. The tocols were eluted under these conditions in the sequence: [30]. The separation of various tocols was also carried out by reverse phase Kinetex 2.6 µ PFP 100A column (150 × 4.6 mm) attached to Security Guard ULTRA Cartridges (UHPLC PFP for 4.6 mm ID column).…”

Section: High Performance Liquid Chromatography Analysismentioning

confidence: 99%

“…The extraction of plasma was carried out for the estimation of α-, β-, γ-, δ-tocopherols and α-, β-, γ-, δ-tocotrienols (tocols) by modified normal and reverse phase HPLC procedures as described [30,31]. Briefly, plasma (200 µl) was added in screw cap disposable glass tube (15 ml) + 200 µl 1% ascorbic acid + 25 µl butylated hydroxyl toluene (1 mg/1 ml) + 900 µl HPLC water + 5 ml HPLC hexane, tube was closed and shaken for 10 minutes on a shaker, and centrifuged for 10 min at 5000 rpm.…”

Section: High Performance Liquid Chromatography Analysismentioning

confidence: 99%

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Pharmacokinetics and Bioavailability of Annatto δ-tocotrienol in Healthy Fed Subjects

Qureshi¹,

Khan²,

Saleem³

et al. 2015

J Clin Exp Cardiolog

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Background: Although, α-tocopherol is the most bioavailable form of vitamin E, but several animal and clinical studies have demonstrated tocotrienol bioavailability to various tissues. There are few reports on bioavailability of tocotrienols in humans. Most studies were carried out with mixtures of tocotrienols + tocopherols rather than pure tocotrienols. Moreover, dietary α-tocopherol interferes with the bioavailability of tocotrienols, and prevents absorption and delivery to organs and tissues.Aim: Pharmacokinetics and bioavailability of annatto-based δ-tocotrienol, plasma levels of α-, β-, γ-, δ-tocotrienol and tocopherols were quantified. In addition, several cytokines and microRNAs were examined. Study design:An open-label, randomized study evaluated pharmacokinetics and bioavailability of δ-tocotrienol in 33 healthy fed subjects. All subjects (11/dose) were randomly assigned to doses of 125, 250, or 500 mg/d. Plasma samples collected at 0, 1, 2, 3, 4, 6, 8, 10 h intervals were estimated by HPLC for tocols (tocotrienols and tocopherols). Results:The present study describes the effects of δ-tocotrienol on pharmacokinetic parameters of all eight tocol isomers. Supplementation of 125, 250 and 500 mg/d doses resulted in dose-dependent increases of (a) area under concentration-time curve (AUC t0 -t10 , ng/ml) 2464, 5412, 14986; (b) maximum concentration (C max , ng/ml) 829, 1920, 3278 (P<0.001); (c) time to achieve maximum peak (T max ; h) 3, 3, 6; (d) elimination of half-life (t 1/2 h) 1.74, 1.39, 2.54; (e) time of clearance (Cl-T, h -1 ) 0.049, 0.045, 0.030; (f) volume of distribution (Vd/f, mg/h) 0.119, 0.114, 0.113; and (g) elimination rate constant (Ke; h -1 ) 0.412, 0.401, 0.265. Similar results were reported for the other tocols. Maximum plasma levels of δ-tocotrienol were observed at 3 h with doses of 125 and 250 mg/d, and 6 h with 500 mg/d. γ-tocotrienol, β-tocotrienol, α-tocotrienol, δ-tocopherol, γ-tocopherol β-tocopherol and α-tocopherol were appeared in the plasma after 2 h. Moreover, δ-tocotrienol treatment resulted in down-regulation of eight cytokines and upregulation of adiponectin, TGF-β1, and leptin. The expression of miR-34a (increased in bipolar disorder) was down-regulated, but expression of miR-107, miR-122a, and miR-132 (decreased in Alzheimer's disease) was upregulated by δ-tocotrienol treatment.Conclusion: This is the first study describing the effect of δ-tocotrienol on pharmacokinetics and bioavailability of all eight tocol isomers. When tocotrienols are supplemented in absence of tocopherols, δ-tocotrienol has better bioavailability, and δ-tocotrienol is converted stepwise to other tocotrienols/tocopherols. These results support that tocotrienol, particularly δ-tocotrienol, as a dietary supplement might be useful in the prevention of age-related and chronic ailments. Pharmacokinetics and Bioavailability of Annatto δ-tocotrienol in Healthy Fed Subjects IntroductionSeveral studies have reported the antioxidant, anti-inflammatory, anticancer, hypocholesterolemic and neuroprotec...

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Section: High Performance Liquid Chromatography Analysismentioning

confidence: 99%

Section: High Performance Liquid Chromatography Analysismentioning

confidence: 99%

Section: High Performance Liquid Chromatography Analysismentioning

confidence: 99%

See 1 more Smart Citation

Pharmacokinetics and Bioavailability of Annatto δ-tocotrienol in Healthy Fed Subjects

Qureshi¹,

Khan²,

Saleem³

et al. 2015

J Clin Exp Cardiolog

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“…Separation of D-D- D-, and D--tocopherol by HPLC has been reported with normal-phase HPLC using silica, amino, and diol stationary phases [3,4], with reversed-phase HPLC using silica-C30, pentafluorophenyl, high-density C18 stationary phase with polymeric bonding [5,6,7,8], and with monolithic columns possessing a polymer stationary phase [9,10]. Normal phase LC separates all natural tocopherol isomers, and is well suited for direct injection of oils, but is not used for complicated biological samples in practice.…”

Section: Introductionmentioning

confidence: 99%

“…Some special stationary phases have been employed for the separation of -and -tocopherolsin reversed-phase liquid chromatography [5][6][7][8]. Two stationary phases, polymeric silica C18 (Inertsil ODS-P) and polymeric C30 (Inertsil C30 S-Select) showed opposite elution orders for  and isomers.…”

Section: Introductionmentioning

confidence: 99%

Distinction of synthetic dl-α-tocopherol from natural vitamin E (d-α-tocopherol) by reversed-phase liquid chromatography. Enhanced selectivity of a polymeric C18 stationary phase at low temperature and/or at high pressure

Yui¹,

Miyazaki²,

et al. 2016

Journal of Chromatography A

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Development and validation of a single HPLC method for determination of α‐tocopherol in cell culture and in human or mouse biological samples

Cimadevilla

Hevia

Miar

et al. 2014

Biomedical Chromatography

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A straightforward and common analytical method for α-tocopherol (αT) determination in various biological samples, including plasma, red blood cells (RBC), tissues and cultured cell lines, was developed and validated, using a reverse phase-chromatographic method (RP-HPLC). Even though many chromatographic methods for αT determination have been reported, most of them require readjustment when applied to different types of samples. Thus, an effective and simple method for αT determination in different biological matrices is still necessary, specifically for translational research. This method was applied using a C18 column (250 × 4.6 mm, 5 µm particle size) under isocratic elution with MeOH:ACN:H2 O (90:9:1 v/v/v) at a flow rate of 1 mL/min and detected using photodiode array at 293 nm. Linearity (r >0.9997) was observed for standard calibration with inter- and intraday variation of standard <4%. Lower limits of detection and quantification for αT in this assay were 0.091 and 0.305 µg/mL respectively. Validation proved the method to be selective, linear, accurate and precise. The method was successfully applied in great variety of biological samples, that is, human and mouse plasma, RBCs, murine tissues and human/mouse/rat cultured cell lines. More importantly, a single protocol of extraction and detection can be applied, making this method very convenient for standardization of different types of samples.

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Rapid baseline-separation of all eight tocopherols and tocotrienols by reversed-phase liquid-chromatography with a solid-core pentafluorophenyl column and their sensitive quantification in plasma and liver

Cited by 118 publications

References 21 publications

Pharmacokinetics and Bioavailability of Annatto δ-tocotrienol in Healthy Fed Subjects

Pharmacokinetics and Bioavailability of Annatto δ-tocotrienol in Healthy Fed Subjects

Distinction of synthetic dl-α-tocopherol from natural vitamin E (d-α-tocopherol) by reversed-phase liquid chromatography. Enhanced selectivity of a polymeric C18 stationary phase at low temperature and/or at high pressure

Development and validation of a single HPLC method for determination of α‐tocopherol in cell culture and in human or mouse biological samples

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