Rapid bidirectional allele‐specific PCR identification for triazine resistance in higher plants

Tian, Xin; Darmency, Henri

doi:10.1002/ps.1197

Cited by 19 publications

(21 citation statements)

References 42 publications

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“…These results confirm the results of Tian and Darmency (2006), Park and MallorySmith (2006) and Elahifard et al (2013a), with a slight difference from Elahifard et al (2013a) in S-biotype sequencing. They reported a serine-to-glycine substitution in codon 282 in addition to the 232 position but the results of the present study found no such substitution at the 282 site for the S and R biotypes.…”

Section: Comparing Paba Gene Sequence In S and R-biotypessupporting

confidence: 90%

Mechanisms of resistance to metribuzin in new resistant biotype of jungle rice (Echinochloa colona) in sugarcane fields of hot semi-arid climates

Nabipour¹,

Ahmadpour²,

Mesgarbashi³

et al. 2017

Aust J Crop Sci

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The practice of monoculture and the use of one-mode-action herbicides for more than 10-year period can cause development of resistant jungle rice [(Echinochloa colona (L.)] to photosystem II inhibitors. Jungle rice is one of the most troublesome weeds in the sugarcane fields of Iran. Laboratory and greenhouse testing was carried out from 2013 to 2016 to investigate the development and determine the origin of metribuzin-resistant jungle rice biotypes in the sugarcane fields of Khuzestan province. Five populations of jungle rice (1 susceptible and 4 suspected resistant) were collected and assayed. The suspected populations were first screened using discriminating concentrations of mertibuzin. To determine the resistance factor for R-biotypes, bioassay trials were conducted in a greenhouse. Then, PCR sequencing markers used in the laboratory and PCR products were subjected to pair-end sequencing by Eurofins Scientific to determine the molecular base of resistance in the R-biotypes. The results of seedling bioassays showed that four biotypes were resistant to metribuzin. The weed control efficiency of metribuzin for the R4, R2, R3 and R1 biotypes were 12.38%, 27.24%, 59.29% and 75.21%, respectively. The resistance factor for the R4 and R2 biotypes were 58.51 and 30.23 respectively; ranking these as resistant biotypes. Gene sequencing studies found an effective mutation at position 232 of the psbA gene that resulted in amino acid substitution from Serine to Glycine at residue 264 in the D1 protein in R-biotypes versus S-biotypes. These results show that target site resistance to metribuzin is rapidly developing in sugarcane fields in northern Khuzestan province. This could stem from the spread of resistant biotype seeds through irrigation water from the Karun Agro-industry fields (first reported sites of resistance) or by herbicide selection pressure.

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Section: Comparing Paba Gene Sequence In S and R-biotypessupporting

confidence: 90%

Mechanisms of resistance to metribuzin in new resistant biotype of jungle rice (Echinochloa colona) in sugarcane fields of hot semi-arid climates

Nabipour¹,

Ahmadpour²,

Mesgarbashi³

et al. 2017

Aust J Crop Sci

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“…Populations of many other weed species also exhibited the same resistance . In most cases, triazine resistance was due to a point mutation (Ser‐264‐Gly) on the chloroplast gene psbA ; psbA encodes for the thylakoid D1 protein, which is critical for photosystem II energy transfer . This mutation results in a conformational change of the D1 protein, though the protein continues to function, otherwise, the electron transfer would be blocked.…”

Section: Triazine Resistancementioning

confidence: 99%

Pleiotropic effects of herbicide-resistance genes on crop yield: a review

Darmency

2013

Pest. Manag. Sci.

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The rapid adoption of genetically engineered herbicide-resistant crop varieties (HRCVs)-encompassing 83% of all GM crops and nearly 8% of the worldwide arable area-is due to technical efficiency and higher returns. Other herbicide-resistant varieties obtained from genetic resources and mutagenesis have also been successfully released. Although the benefit for weed control is the main criteria for choosing HRCVs, the pleiotropic costs of genes endowing resistance have rarely been investigated in crops. Here the available data of comparisons between isogenic resistant and susceptible varieties are reviewed. Pleiotropic harmful effects on yield are reported in half of the cases, mostly with resistance mechanisms that originate from genetic resources and mutagenesis (atrazine in oilseed rape and millet, trifluralin in millet, imazamox in cotton) rather than genetic engineering (chlorsulfuron and glufosinate in some oilseed rape varieties, glyphosate in soybean). No effect was found for sethoxydim and bromoxynil resistance. Variable minor effects were found for imazamox, chlorsulfuron, glufosinate and glyphosate resistance. The importance of the breeding plan and the genetic background on the emergence of these effects is pointed out. Breeders' efforts to produce better varieties could compensate for the yield loss, which eliminates any possibility of formulating generic conclusions on pleiotropic effects that can be applied to all resistant crops.

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“…Tian and Darmency (2006) identified a point mutation related to triazine resistance in higher plants, which caused a Ser 264 -Gly substitution that confered resistance in Setaria italica. Park and Mallory-Smith (2006) analyzed the nucleotidic sequence in one bromoxynil-resistant S. vulgaris biotype and identified a point mutation which induced the amino acid substitution from asparagine to threonine at codon 266.…”

Section: Analysis Of the Sequenced Ampliconsmentioning

confidence: 99%

Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn

et al. 2010

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ABSTRACT. Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction -Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psbA gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn. RESUMO. Caracterização molecular de acessos de capim-colchão (Digitaria nuda) e resposta à ametrina. As espécies de Digitaria são plantas daninhas nas lavouras de cana-deaçúcar no Brasil e estão sendo controladas com herbicidas, porém há alguns relatos de falha no controle, notavelmente ao grupo das triazinas. Técnicas moleculares são recomendadas para análise da variabilidade genética em plantas daninhas, como o RAPD (DNA Polimórfico Amplificado ao Acaso) e PCR-RFLP (Reação da Polimerase em Cadeia -Polimorfismo no Comprimento de Fragmentos de Restrição) e, aliadas ao sequenciamento, permitem a localização de genes de resistência, bem como possíveis mutações relacionadas ao surgimento de indivíduos resistentes em algumas espécies. Com isso, o objetivo deste trabalho foi caracterizar dez acessos de Digitaria spp. por meio de marcadores RAPD e PCR-RFLP, sequenciar uma região conservada do gene psbA e analisar a resposta destes à ametrina. Pela análise molecular houve elevada similaridade genética entre os acessos, pois todos apresentaram perfis genéticos semelhantes e foram suscetíveis à ametrina.

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Rapid bidirectional allele‐specific PCR identification for triazine resistance in higher plants

Cited by 19 publications

References 42 publications

Mechanisms of resistance to metribuzin in new resistant biotype of jungle rice (Echinochloa colona) in sugarcane fields of hot semi-arid climates

Mechanisms of resistance to metribuzin in new resistant biotype of jungle rice (Echinochloa colona) in sugarcane fields of hot semi-arid climates

Pleiotropic effects of herbicide-resistance genes on crop yield: a review

Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn

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