Rapid biodegradation of aflatoxin <scp>B1</scp> by metabolites of <i>Fusarium</i> sp. <scp>WCQ3361</scp> with broad working temperature range and excellent thermostability

Wang, Cuiqiong; Li, Zhongyuan; Hui, Wu; Qiu, Haiyan; Zhang, Minghui; Li, Shuang; Luo, Xue‐Gang; Song, Yajian; Zhou, Hao; Ma, Wenjian; Zhang, Tongcun

doi:10.1002/jsfa.7872

Cited by 31 publications

(30 citation statements)

References 26 publications

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“…However, Fusarium sp. WCQ3361 metabolites displayed excellent thermostability, since temperature change (0–90 °C) did not significantly affect the AFB 1 degradation activity [ 23 ]. Similarly, the culture supernatant of DY3108 showed excellent thermostability, since the degradation rate increased with increasing temperature up to 80 °C.…”

Section: Resultsmentioning

confidence: 99%

“…Kavitha et al [ 34 ] isolated an antifungal protein with robust thermal stability from Bacillus polymyxa strain VLB16, which still maintained activity after sterilization (15 min at 121 °C). In addition, Wang et al [ 23 ] found that the metabolites of Fusarium sp. WCQ3361 exhibited high thermal stability and 99.40% residual activity to degrade AFB 1 was retained even after boiling for 10 min.…”

Section: Resultsmentioning

confidence: 99%

“…Despite these fungi and bacteria were able to degrade AFB 1 effectively, few strains have been applied commercially because the actual use of these microorganisms or their metabolites were affected by the long reaction times, narrow working temperatures, or relatively low degradative efficiency. Therefore, exploring microorganisms or their metabolites that degrade and detoxify AFB 1 with excellent degradation efficacy, wide temperature ranges, or short degradation times would be highly beneficial [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability

Shu

Wang

Zhou

et al. 2018

Toxins

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(1) Background: Aflatoxin contamination in food and grain poses serious problems both for economic development and public health protection, thus leading to a focus on an effective approach to control it; (2) Methods: Aflatoxin B1 (AFB1) degrading bacteria were isolated using a medium containing coumarin as the sole carbon source, and the biodegradation of AFB1 by the isolate was examined by high performance liquid chromatography, and liquid chromatography mass spectrometry; (3) Results: a bacterial strain exhibiting strong AFB1 degradation activity (91.5%) was isolated and identified as Bacillus velezensis DY3108. The AFB1 degrading activity was predominantly attributed to the cell-free supernatant of strain DY3108. Besides, it was heat-stable and resistant to proteinase K treatment but sensitive to sodium dodecyl sulfate treatment. The optimal temperature for the maximal degradation of AFB1 was 80 °C. Even more notable, the supernatant showed a high level of activity over a broad pH (4.0 to 11.0) and exhibited the highest degradation (94.70%) at pH 8.0. Cytotoxicity assays indicated that the degradation products displayed significantly (p < 0.05) lower cytotoxic effects than the parent AFB1; (4) Conclusions: B. velezensis DY3108 might be a promising candidate for exploitation in AFB1 detoxification and bioremediation in food and feed matrices.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability

Shu

Wang

Zhou

et al. 2018

Toxins

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“…However, only a few of these methods conform to the Food and Agriculture Organization’s (FAO) requirement that the reduction of AFB1 is achieved while maintaining the nutritional value without residual toxicity or modifying the food or feed properties [6,7]. Biological degradation has proven to be an efficient, safe, and feasible method for AFB1 removal because its raw materials are non-polluting, highly specific, mild, and environmentally friendly; this process also discourages toxin regeneration [8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain

Wang

Zhang

Yan

et al. 2018

Toxins

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Aflatoxin B1 (AFB1), a pollutant of agricultural products, has attracted considerable attention in recent years, due to its potential impact on health. In the present study, Bacillus licheniformis (BL010) was demonstrated to efficiently degrade AFB1, reducing over 89.1% of the toxin content within 120 h. A crude enzyme solution of BL010 exhibited the highest degradation level (97.3%) after three induction periods. However, uninduced BL010 bacteria was not capable of reducing AFB1. Furthermore, high performance liquid chromatography (HPLC) analysis showed that while a cell-free extract caused a significant decrease in AFB1 content (93.6%, p < 0.05), cell culture fluid treatment did not significantly degrade AFB1. The biotransformation products of AFB1 were detected and further identified by quadrupole time-of-flight liquid chromatography–mass spectrometry (LC-Q-TOF/MS); these corresponded to a molecular formula of C12H14O4. A sequence analysis of whole BL010 genes with a bioinformatics approach identified the secondary structures of two degrading enzymes (Chia010 and Lac010), providing an important basis for subsequent homology modeling and functional predictions.

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“…Pseudomonas spp. Stenotrophomonas spp., Arthrobacter spp., and members of the family Flavobacteriaceae were shown in vitro to produce aflatoxin-degrading enzymes causing a significant reduction (>90%) in the levels of AFB1, AFM1 and/or AFB2 [217][218][219][220]. However, for these microorganisms to be effective in vivo, they have to colonize the intestinal tract and express sufficient levels of the aflatoxin-degrading enzymes.…”

Section: Reducing the Risk By Interfering With The Bioavailability Ofmentioning

confidence: 99%

Retrospective and Prospective Look at Aflatoxin Research and Development from a Practical Standpoint

Benkerroum¹

2019

Preprint

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Among the array of structurally and toxicologically diverse mycotoxins, aflatoxins have attracted the most interest of scientific research due to their high toxicity and incidence in foods and feeds. Despite the undeniable progress made in various aspects related to aflatoxins, the ultimate goal consisting of reducing the associated public health risks worldwide is far from being reached due to multiplicity of social, political, economic, geographic, climatic, and development factors. However, a reasonable degree of health protection is attained in industrialized countries owing to their scientific, administrative, and financial capacities allowing them to use high-tech agricultural management systems. Less fortunate situation exists in equatorial and sub-equatorial developing countries mainly practicing traditional agriculture managed by smallholders for subsistence, and where the climate is suitable for mould growth and aflatoxin production. Accordingly, it is difficult to harmonize the regulatory standards of aflatoxins worldwide, which prevents agri-foods of developing countries from access to the market of industrialized countries. To tackle the multi-facetted aflatoxin problems, actions should be taken collectively by the international community involving scientific research, technological and social development, environment protection, etc. International cooperation should foster technology transfer and exchange of pertinent technical information. This review presents the main historical discoveries leading to our present knowledge on aflatoxins and the challenges that should be addressed presently and in the future at various levels to ensure higher health protection for everybody. In short, it aims to elucidate where we come from and where we should go in terms of aflatoxin research/development.

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Rapid biodegradation of aflatoxin B1 by metabolites of Fusarium sp. WCQ3361 with broad working temperature range and excellent thermostability

Cited by 31 publications

References 26 publications

Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability

Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability

Effective Biodegradation of Aflatoxin B1 Using the Bacillus licheniformis (BL010) Strain

Retrospective and Prospective Look at Aflatoxin Research and Development from a Practical Standpoint

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