Rapid biodegradation of organophosphorus pesticides by Stenotrophomonas sp. G1

Deng, Shuyan; Chen, Yao; Wang, Daosheng; Shi, Taozhong; Wu, Xiangwei; Ma, Xiaoli; Li, Xiangqiong; Hua, Rimao; Tang, Xinyun; Li, Qing X.

doi:10.1016/j.jhazmat.2015.04.052

Cited by 186 publications

(76 citation statements)

References 23 publications

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“…In contrast, strain 5B degraded the highest concentration of fosthiazate (500 mg l À1 ) at a slower rate (DT 50 = 20Á3 days). The slower degradation of the highest concentration of fosthiazate by strain 5B is in line with previous studies which have also observed a retardation or complete halting of pesticide biodegradation at high concentration levels (Rani et al 2008;Anwar et al 2009;Deng et al 2015;Perruchon et al 2016). The dissipation of fosthiazate proceeded at a slower rate in the noninoculated control cultures (abiotic controls) with DT 50s ranging from 33Á4 (20 mg l À1 concentration) to 51Á4 (500 mg l À1 concentration) days.…”

Section: Degradation Of Different Concentrations Of Fosthiazate By Thsupporting

confidence: 89%

Isolation and characterization of soil bacteria able to rapidly degrade the organophosphorus nematicide fosthiazate

Lagos

Perruchon

Katsoula

et al. 2019

Lett Appl Microbiol

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Foshtiazate is an organophosphorus nematicide commonly used in protected crops and potato plantations. It is toxic to mammals, birds and honeybees, it is persistent in certain soils and can be transported to water resources. Recent studies by our group demonstrated, for the first time, the development of enhanced biodegradation of fosthiazate in agricultural soils. However, the micro‐organisms driving this process are still unknown. We aimed to isolate soil bacteria responsible for the enhanced biodegradation of fosthiazate and assess their degradation potential against high concentrations of the nematicide. Enrichment cultures led to the isolation of two bacterial cultures actively degrading fosthiazate. Denaturating Gradient Gel Electrophoresis analysis revealed that they were composed of a single phylotype, identified via 16S rRNA cloning and phylogenetic analysis as Variovorax boronicumulans. This strain showed high degradation potential against fosthiazate. It degraded up to 100 mg l−1 in liquid cultures (DT50 = 11·2 days), whereas its degrading capacity was reduced at higher concentration levels (500 mg l−1, DT50 = 20 days). This is the first report for the isolation of a fosthiazate‐degrading bacterium, which showed high potential for use in future biodepuration and bioremediation applications. Significance and Impact of the Study This study reported for the first time the isolation and molecular identification of bacteria able to rapidly degrade the organophosphorus nematicide fosthiazate; one of the few synthetic nematicides still available on the global market. Further tests demonstrated the high capacity of the isolated strain to degrade high concentrations of fosthiazate suggesting its high potential for future bioremediation applications in contaminated environmental sites, considering high acute toxicity and high persistence and mobility of fosthiazate in acidic and low in organic matter content soils.

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Section: Degradation Of Different Concentrations Of Fosthiazate By Thsupporting

confidence: 89%

Isolation and characterization of soil bacteria able to rapidly degrade the organophosphorus nematicide fosthiazate

Lagos

Perruchon

Katsoula

et al. 2019

Lett Appl Microbiol

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“…There was greater abundance of the above-mentioned species in the BFC and BFN treatments compared to the CF and CN treatments. In some niches, Stenotrophomonas can degrade a variety of xenobiotic compounds [45], such as chlorpyrifos, methyl parathion, methyl paraoxon, diazinon, phoxim, parathion, profenofos, and triazophos [46]. Thus, Stenotrophomonas plays a significant role in the bioremediation of polluted sites, especially those with Cr [47].…”

Section: Discussionmentioning

confidence: 99%

Changes in soil bacterial community structure as a result of incorporation of Brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses

Liu

Zheng

et al. 2017

PLoS ONE

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Greenhouse eggplant monocropping in China has contributed to the aggravation of soil-borne diseases, reductions in crop quality and yield, and the degradation of physical and chemical soil properties. Crop rotation is one effective way of alleviating the problems of continuous cropping worldwide; however, few studies have reported changes in soil bacterial community structures and physical and chemical soil properties after Brassica vegetables had been rotated with eggplant in greenhouses. In this experiment, mustard-eggplant (BFN) and oilseed rape-eggplant (BFC) rotations were studied to identify changes in the physicochemical properties and bacterial community structure in soil that was previously subject to monocropping. Samples were taken after two types of Brassica plants incorporated into soil for 15 days to compare with continually planted eggplant (control, CN) and chemical disinfection of soil (CF) in greenhouses. MiSeq pyrosequencing was used to analyze soil bacterial diversity and structure in the four different treatments. A total of 55,129 reads were identified, and rarefaction analysis showed that the soil treatments were equally sampled. The bacterial richness of the BFC treatment and the diversity of the BFN treatment were significantly higher than those of the other treatments. Further comparison showed that the bacterial community structures of BFC and BFN treatments were also different from CN and CF treatments. The relative abundance of several dominant bacterial genera in the BFC and BFN treatments (such as Flavobacteria, Stenotrophomonas, Massilia and Cellvibrio, which played different roles in improving soil fertility and advancing plant growth) was distinctly higher than the CN or CF treatments. Additionally, the total organic matter and Olsen-P content of the BFC and BFN treatments were significantly greater than the CN treatment. We conclude that Brassica vegetables-eggplant crop rotations could provide a more effective means of solving the problems of greenhouse eggplant monocultures.

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“…resinovorans (12), Ps. fluorescens (13), Bacillus subtilis (13,14,15,16), Bacillus sp and Micrococcus sp (17,18), B. firmis (19), B. cereus (20), Stenotrophomonas (21), Serratia marcescens, Klebsiella oxytoca (22), Achromobacter sp, Ochrobactrum sp (23), Alcaligenes faecalis (24), Mesorhizobium (25), cellulomonas fimi (26), Gordonia (27), B. polymyxa (28), Kocuria sp (29), Staphylococcus sp, Streptococcus sp, azomonas sp, Flavobacterium sp (30) were extensively studied in degradation of chlorpyrifos.…”

Section: Issn: 2320-5407mentioning

confidence: 99%

Isolation and Identification of Chlorpyrifos Degrading Bacteria From Agricultural Soil.

Hamsavathani¹,

Aysha²,

Ajith³

2017

IJAR

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The extensive use of pesticides in agricultural fields for pest control pose a serious problem in contaminating soil and water ecosystems. Chlorpyrifos is the major broad spectrum organophosphorus insecticide used in paddy fields against sucking, chewing, boring insects. Due to its toxicity and persistence in the environment, there is an immediate need to eliminate them from contaminated sites by biodegradation. Two pesticide degrading bacteria were screened and isolated from chlorpyrifos contaminated soil by enrichment culture technique and were identified as Kocuria kristinae and Staphylococcus aureus. The growth response and degradation of chlorpyrifos by the isolates in MSM broth supplemented with 0.5% chlorpyrifos was monitored every 48-72 hrs in spectrophotometer at 600nm. Kocuria sp showed maximum growth in 7 days than Staphylococcus aureus. The degradation efficiency of the strains were determined and estimated by the removal percentage of chlorpyrifos from the liquid culture. Both the isolate showed the degrading capability of chlorpyrifos in MSM. The isolate, S. aureus was more potent in degrading the 80% of the total compound from the media in 2 weeks of incubation than K. kristinae which shows 35 % of degradation. These results were further confirmed by GCMS, in which S. aureus has degraded 82.06% and K. kristinae has degraded 30.78% of chlorpyrifos in the medium. This study indicates that the isolate, Staphylococcus aureus is more potent in degrading chlorpyrifos in liquid culture and can also be used in bioremediation of chlorpyrifos contaminated soils.

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Rapid biodegradation of organophosphorus pesticides by Stenotrophomonas sp. G1

Cited by 186 publications

References 23 publications

Isolation and characterization of soil bacteria able to rapidly degrade the organophosphorus nematicide fosthiazate

Isolation and characterization of soil bacteria able to rapidly degrade the organophosphorus nematicide fosthiazate

Changes in soil bacterial community structure as a result of incorporation of Brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses

Isolation and Identification of Chlorpyrifos Degrading Bacteria From Agricultural Soil.

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