Rapid biomimetic mineralization of collagen fibrils and combining with human umbilical cord mesenchymal stem cells for bone defects healing

Ye, Bihua; Luo, Xueshi; Li, Zhiwen; Zhuang, Caiping; Li, Lihua; Lu, Lu; Ding, Shan; Tian, Jie; Zhou, Changren

doi:10.1016/j.msec.2016.05.104

Cited by 34 publications

(35 citation statements)

References 33 publications

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“…Some bone substitutes in nanofibers containing these two biomolecules, when subjected to cellular activity, can gradually release these ions, aiding in bone regeneration (Bouler et al, ; Yokote et al, ; Zhang et al, ). The ACP fibers were thus idealized for this study due to their surface topography and surface degradation, allowing a better natural biomineralization of newly deposited tissues using calcium and phosphate ions dispersed in the extracellular medium (Ye et al, ).…”

Section: Discussionmentioning

confidence: 99%

Amorphous calcium phosphate (ACP) in tissue repair process

Allegrini

Silva

Tsujita

et al. 2018

Microscopy Res & Technique

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Synthetic biomaterials submitted to new structural technologies have become ideal for the recovery of traumatized bone tissues and some bone substitutes such as bioactive glass, β-Tricalcium phosphate (β-TCP) and amorphous calcium phosphate (ACP) are being used in areas of tissue defects. For this study, ACP was produced in the form of fibers and then submitted to cytotoxicity testing. A sample of ACP was inserted into the mandibular region of a patient with a lost implant so after removal and curettage, the remaining bone site was filled with the ACP biomaterial. Preliminary cytotoxicity test was negative. After 15 weeks of healing, a titanium implant was inserted at the site. Clinical and radiographic follow-up was conducted for 12 months and sequential radiographic analyses revealed tissue formation resembling spongy bone. Images under immunohistochemistry demonstrated efficient deposition and osteoconduction of the newly deposited tissue. Residual portion of the CaO:P O outer layers served as a substrate for osteoid matrix deposition, aiding growth, and the results of fiber absorption favored maturation of the new bone tissue.

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Section: Discussionmentioning

confidence: 99%

Amorphous calcium phosphate (ACP) in tissue repair process

Allegrini

Silva

Tsujita

et al. 2018

Microscopy Res & Technique

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“…Drawbacks of autografting including the limited supply and donor site morbidity make the preparation of osteoinductive biomaterials as autograft substitutes an area of intense research . The currently used bone substitutes such as calcium‐phosphate ceramics and mineralized collagen composites try to simulate the mineral composition or the structure of interconnected struts of natural bone . However, their utility are restricted by variable degradation rates, brittle or insufficient strength, and limited intrinsic osteoinductivity .…”

Section: Introductionmentioning

confidence: 99%

“…The currently used bone substitutes such as calcium‐phosphate ceramics and mineralized collagen composites try to simulate the mineral composition or the structure of interconnected struts of natural bone . However, their utility are restricted by variable degradation rates, brittle or insufficient strength, and limited intrinsic osteoinductivity . Improved autograft substitutes are needed to mimic bone hierarchy and surface topography, and match the performance of autografts to provide an optimal microenvironment for host stem cell recruitment and differentiation …”

Section: Introductionmentioning

confidence: 99%

Thermodynamically Controlled Self‐Assembly of Hierarchically Staggered Architecture as an Osteoinductive Alternative to Bone Autografts

Liu

Luo

et al. 2019

Adv Funct Materials

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Osteoinductive synthetic biomaterials for replacing autografts can be developed by mimicking bone hierarchy and surface topography for host cell recruitment and differentiation. Until now, it has been challenging to reproduce a bone‐like staggered hierarchical structure since the energy change underlying synthetic pathways in vitro is essentially different from that of the natural process in vivo. Herein, a bone‐like hierarchically staggered architecture is reproduced under thermodynamic control involving two steps: fabrication of a high‐energy polyacrylic acid‐calcium intermediate and selective mineralization in collagenous gap regions driven by an energetically downhill process. The intermediate energy interval could easily be adjusted to determine different mineralization modes, with distinct morphologies and biofunctions. Similar to bone autografts, the staggered architecture offers a bone‐specific microenvironment for stem cell recruitment and multidifferentiation in vitro, and induces neo‐bone formation with bone marrow blood vessels by host stem cell homing in vivo. This work provides a novel perspective for an in vitro simulating biological mineralization process and proof of concept for the clinical application of smart biomaterials.

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“…Therefore, it is believed that hUCMSCs own the characteristics of both seed cells and cytokines in the process of fabrication of tissue‐engineered bone, which will be a promising choice to enhance the bioactivity of tissue‐engineered bone graft . Numerous researches have also shown that hUCMSCs have superior osteogenic properties attached to the scaffolds and are suitable seed cells for bone tissue engineering …”

Section: Introductionmentioning

confidence: 99%

A novel method to improve the osteogenesis capacity of hUCMSCs with dual‐directional pre‐induction under screened co‐culture conditions

Rong

Zhou

et al. 2019

Cell Proliferation

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Objectives Mesenchymal stem cells (MSCs) based therapy for bone regeneration has been regarded as a promising method in the clinic. However, hBMSCs with invasive harvesting process and undesirable proliferation rate hinder the extensive usage. HUCMSCs of easier access and excellent performances provide an alternative for the fabrication of tissue‐engineered bone construct. Evidence suggested the osteogenesis ability of hUCMSCs was weaker than that of hBMSCs. To address this issue, a co‐culture strategy of osteogenically and angiogenically induced hUCMSCs has been proposed since thorough vascularization facilitates the blood‐borne nutrition and oxygen to transport in the scaffold, synergistically expediting the process of ossification. Materials and methods Herein, we used osteogenic‐ and angiogenic‐differentiated hUCMSCs for co‐culture in screened culture medium to elevate the osteogenic capacity with in vitro studies and finally coupled with 3D TCP scaffold to repair rat's critical‐sized calvarial bone defect. By dual‐directional induction, hUCMSCs could differentiate into osteoblasts and endothelial cells, respectively. To optimize the co‐culture condition, gradient ratios of dual‐directional differentiated hUCMSCs co‐cultured under different medium were studied to determine the appropriate condition. Results It revealed that the osteogenic‐ and angiogenic‐induced hUCMSCs mixed with the ratio of 3:1 co‐cultured in the mixed medium of osteogenic induction medium to endothelial cell induction medium of 3:1 possessed more mineralization nodules. Similarly, ALP and osteogenesis/angiogenesis‐related genes expressions were relatively higher. Further evidence of bone defect repair with 3D printed TCP of 3:1 group exhibited better restoration outcomes. Conclusions Our work demonstrated a favourable and convenient approach of dual‐directional differentiated hUCMSCs co‐culture to improve the osteogenesis, establishing a novel way to fabricate tissue‐engineered bone graft with 3D TCP for large bone defect augmentation.

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Rapid biomimetic mineralization of collagen fibrils and combining with human umbilical cord mesenchymal stem cells for bone defects healing

Cited by 34 publications

References 33 publications

Amorphous calcium phosphate (ACP) in tissue repair process

Amorphous calcium phosphate (ACP) in tissue repair process

Thermodynamically Controlled Self‐Assembly of Hierarchically Staggered Architecture as an Osteoinductive Alternative to Bone Autografts

A novel method to improve the osteogenesis capacity of hUCMSCs with dual‐directional pre‐induction under screened co‐culture conditions

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