Rapid biosynthesis of silver nanoparticles from Bacillus megaterium (NCIM 2326) and their antibacterial activity on multi drug resistant clinical pathogens

Saravanan, Muthupandian; Vemu, Anil Kumar; Barik, Sisir Kumar

doi:10.1016/j.colsurfb.2011.07.009

Cited by 164 publications

(90 citation statements)

References 58 publications

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“…Such impressive effect of AgNPs may be due to interplay with the thiol group of L-cysteine protein residues that may force to enzymatic dysfunction [29] or the AgNPs may have galvanize the release of reactive oxygen species, which lead to destruction of DNA and protein of microorganism [30]. Furthermore, it is found that AgNPs have higher antibacterial activities (Comparatively higher MIC against Gram(+) bacteria) against Gram(-) bacteria may be due to the absence of thick layer of peptidoglycan in cell wall of Gram (-) bacteria [31,32].…”

Section: Minimum Inhibitory Concentration and Minimal Bactericidal Comentioning

confidence: 99%

Synthesis, Characterization and Antimicrobial Activity of Abelia grandiflora Assisted AgNPs

Sharma¹,

Jasuja²,

Rajgovind³

et al. 2014

Microb Biochem Technol

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In past few years, wide applicability of silver nanoparticles (AgNPs) in various field attract for an approach of rapid, cost effective and eco-friendly synthesis of AgNPs that is expanding research toward biological methods. The biosynthesised AgNPs were confirmed visually by appearance of dark brown color formation in mixture and silver surface plasmon resonance band observed at 413 nm by using UV-Visible Spectroscopy A. grandiflora may be used for the green synthesis of ultra-fine nanoparticles of silver for their antimicrobial activities.

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Section: Minimum Inhibitory Concentration and Minimal Bactericidal Comentioning

confidence: 99%

Synthesis, Characterization and Antimicrobial Activity of Abelia grandiflora Assisted AgNPs

Sharma¹,

Jasuja²,

Rajgovind³

et al. 2014

Microb Biochem Technol

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“…As a result, the Gram-positive bacteria are defended from the bactericidal mechanisms, which are released by AgNPs. 53,54 cytotoxicity of silver nanoparticles…”

Section: Tem and Edx Analysismentioning

confidence: 99%

Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study

Arokiyaraj¹,

Arasu²,

Vincent³

et al. 2014

IJN

188

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The present work reports a simple, cost-effective, and ecofriendly method for the synthesis of silver nanoparticles (AgNPs) using Chrysanthemum indicum and its antibacterial and cytotoxic effects. The formation of AgNPs was confirmed by color change, and it was further characterized by ultraviolet–visible spectroscopy (435 nm). The phytochemical screening of C. indicum revealed the presence of flavonoids, terpenoids, and glycosides, suggesting that these compounds act as reducing and stabilizing agents. The crystalline nature of the synthesized particles was confirmed by X-ray diffraction, as they exhibited face-centered cubic symmetry. The size and morphology of the particles were characterized by transmission electron microscopy, which showed spherical shapes and sizes that ranged between 37.71–71.99 nm. Energy-dispersive X-ray spectroscopy documented the presence of silver. The antimicrobial effect of the synthesized AgNPs revealed a significant effect against the bacteria Klebsiella pneumonia, Escherichia coli , and Pseudomonas aeruginosa . Additionally, cytotoxic assays showed no toxicity of AgNPs toward 3T3 mouse embryo fibroblast cells (25 μg/mL); hence, these particles were safe to use.

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“…These materials have good antibacterial efficiency. [21][22][23][24] Photodynamic antimicrobial chemotherapy (PACT) is a promising method to eradicate pathogenic bacteria, because it kills these via cytotoxic reactive oxygen species (ROS). ROS are produced by the photosensitive drug after light irradiation, and inflict aspecific damage to bacteria.…”

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confidence: 99%

Photodynamic antimicrobial chemotherapy for <em>Staphylococcus aureus</em> and multidrug-resistant bacterial burn infection in vitro and in vivo

Mai¹,

Gao²,

Li³

et al. 2017

IJN

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Background and objectives Antibiotic resistance has emerged as one of the most important determinants of outcome in patients with serious infections, along with the virulence of the underlying pathogen. Photodynamic antimicrobial chemotherapy (PACT) has been proposed as an alternative approach for the inactivation of bacteria. This study aims to evaluate the antibacterial effect of sinoporphyrin sodium (DVDMS)-mediated PACT on Staphylococcus aureus and multidrug resistant S. aureus in vitro and in vivo. Materials and methods Bacteria were incubated with DVDMS and exposed to treatment with light. After PACT treatment, colony-forming units were counted to estimate the bactericidal effect. Intracellular reactive oxygen-species production was detected by flow cytometry. Flow cytometry and fluorescence-microscopy detection of bacterial cell-membrane permeability. Enzyme-linked immunosorbent assays were used to determine expression of VEGF, TGFβ 1 , TNFα, IL6, and bFGF factors in burn infection. Results DVDMS-PACT effectively killed bacterial proliferation. Intracellular ROS levels were enhanced obviously in the PACT-treatment group. SYTO 9 and propidium iodide staining showed a decrease in the ratio of green:red fluorescence intensity in the PACT-treatment group in comparison to the control group. Enzyme-linked immunosorbent-assay results revealed that in the healing process, the expression of bFGF, TGFβ 1 , and VEGF in the treatment group were higher than in the control group, which inhibited inflammation-factor secretion. In addition, skin-tissue bacteria were reduced after treatment. Conclusion These results indicate that DVDMS-PACT presents significant bactericidal activity and promotes wound healing after burn infections.

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Rapid biosynthesis of silver nanoparticles from Bacillus megaterium (NCIM 2326) and their antibacterial activity on multi drug resistant clinical pathogens

Cited by 164 publications

References 58 publications

Synthesis, Characterization and Antimicrobial Activity of Abelia grandiflora Assisted AgNPs

Synthesis, Characterization and Antimicrobial Activity of Abelia grandiflora Assisted AgNPs

Rapid green synthesis of silver nanoparticles from Chrysanthemum indicum L and its antibacterial and cytotoxic effects: an in vitro study

Photodynamic antimicrobial chemotherapy for <em>Staphylococcus aureus</em> and multidrug-resistant bacterial burn infection in vitro and in vivo

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