Rapid capillary electrophoresis analysis of glutathione and glutathione disulfide in roots and shoots of plants exposed to copper

Mendoza, Jorge; Garrido, Tatiana; Riveros, Raúl; Parada, José

doi:10.1002/pca.1105

Cited by 17 publications

(13 citation statements)

References 19 publications

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“…Capillary zone electrophoresis was used by various researchers (Kubalczyk & Bald, 2009;Li, Cheng & Tseng, 2009;Mendoza et al, 2009;Yan et al, 2009;Chang & Tseng, 2010;Shen, Tseng & Hsieh, 2012;Tsardaka et al, 2013;Wang et al, 2014a;Wang et al, 2014b), but it does not appear to offer a specific advantage in terms of sensitivity when used alone without derivatization (Kubalczyk & Bald, 2009;Mendoza et al, 2009;Yan et al, 2009;Tsardaka et al, 2013;Wang et al, 2014a;Wang et al, 2014b). However, it surely allowed shorter analysis times.…”

Section: Capillary Electrophoresis In Gsh Determinationmentioning

confidence: 99%

Analytical methods for determination of glutathione and glutathione disulfide in pharmaceuticals and biological fluids

Hamad

Elshahawy

Negm

et al. 2020

Reviews in Analytical Chemistry

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AbstractGlutathione is a natural tripeptide that plays a major role in different physiological processes in the human body. Determination of glutathione in different body fluids and tissues is essential for early diagnosis and follow-up of various diseases. The assay of glutathione is problematic because of the high polarity, the limited stability, and the aliphatic structure, which lacks the appropriate chromophore for UV detection. A number of methods have been reported for determination of glutathione using different techniques. High-performance liquid chromatography was employed in both reverse phase and hydrophilic interaction modes. Electrochemical methods exploited the redox activity of glutathione to allow for quantification by different electrodes after chemical modification, including glassy carbon, carbon paste, and nanocomposite electrodes. Capillary zone electrophoresis was used with less need for derivatization which makes it simpler, faster, and more economic. A number of nanosensors and probes have been developed to assay glutathione in biological fluids using semiconductor nanoparticles, quantum dots, genetically engineered green fluorescent probes, and new derivatives of known dye classes. This work is an updated review of the methods of analysis of glutathione and glutathione disulfide in pharmaceuticals and biological fluids with more emphasis on the technical problems and the assay artifacts.

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Section: Capillary Electrophoresis In Gsh Determinationmentioning

confidence: 99%

Analytical methods for determination of glutathione and glutathione disulfide in pharmaceuticals and biological fluids

Hamad

Elshahawy

Negm

et al. 2020

Reviews in Analytical Chemistry

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show abstract

“…Combined multidimensional UV‐photometric, fluorometric and contactless conductometric single point detector was developed using light emitting diodes at 255 or 470 nm as light sources, optical fibers as light guides and a miniature photomultiplier tube (for 255 nm) or silicone photodiode with preamplifier (for 470 nm) as detectors for photometric and fluorescence detection modes, respectively 164. Two 10 mm long metal tubes with id 0.406 mm and 7 mm gap between them served as electrodes of contacless conductivity detector.…”

Section: Detectionmentioning

confidence: 99%

“…CZE with UV‐detection at ultralow UV detection wavelength 185 nm enhancing sensitivity of this detection mode has been developed for fast simultaneous analysis of GSH and GSSG in the roots and shoots of tomato plants grown under normal or stressful (exposed to copper) conditions 164. Using 300 mM borate BGE, pH 7.6, in bare FS capillary (75 μm id, 40/30 cm) and 25 kV separation voltage the separation of GSH and GSSG was achieved within 3 min.…”

Section: Applicationsmentioning

confidence: 99%

Recent developments in CE and CEC of peptides (2009–2011)

Kašička

2011

Electrophoresis

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The review brings a comprehensive survey of the recent developments of high-performance electroseparation methods in capillary and microchip formats: zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography and electrochromatography. Applications of these techniques to analysis, isolation, purification and physicochemical and biochemical characterization of peptides are described. Advances in the investigation of electromigration properties of peptides, and in the methodology of their analysis, such as sample preparation, adsorption suppression, EOF control and detection, are presented. New developments, in particular, CE and CEC modes are reported and several types of their applications to peptide analysis are described: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide relevant physicochemical characteristics of peptides are demonstrated.

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“…7 Therefore, it is very important to monitor the GSH concentration. To date, various methods for measuring GSH have been developed, including fluorescence-electroanalysis (FL-EC), 8 liquid chromatography (LC), 9 liquid chromatography-mass spectrometry (LC-MS), 10 capillary electrophoresis (CE), 11 electroanalysis (EC), 12 and electrochemiluminescence (ECL). 13 Conventional methods for detecting the cellular GSH concentration typically utilize thiol-sensitive organic fluorophores.…”

Section: Introductionmentioning

confidence: 99%

Cobalt Oxyhydroxide-prompted Synthesis of Fluorescent Polydopamine Nanoparticles for Glutathione Detection

et al. 2019

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Glutathione (GSH) plays an important role in cells, which is an essential endogenous antioxidant. Here, we have developed a new detection platform to analyze GSH levels. In our system, fluorescent polydopamine (PDA) nanoparticles, as signal indicators, were obtained by oxidation through cobalt oxyhydroxide (CoOOH) nanosheets. When CoOOH was present, CoOOH could quickly oxidize dopamine to fluorescent PDA nanoparticles. However, once GSH existed, CoOOH nanosheets were decomposed into Co 2+ , and oxidation between CoOOH and dopamine was prevented with weaker fluorescence occurring. Thus, we could realize detection of the GSH concentration according to the decreased fluorescence value of the fluorescent polydopamine. This method provides a fast, simple, high sensitivity and desirable selectivity platform for GSH monitoring.

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Rapid capillary electrophoresis analysis of glutathione and glutathione disulfide in roots and shoots of plants exposed to copper

Abstract: The results obtained here support the applicability of the method to the fast and simultaneous determination of glutathione and glutathione disulfide in tissue of shoots and roots of plants grown under either normal or stressful conditions.

Cited by 17 publications

References 19 publications

Analytical methods for determination of glutathione and glutathione disulfide in pharmaceuticals and biological fluids

Analytical methods for determination of glutathione and glutathione disulfide in pharmaceuticals and biological fluids

Recent developments in CE and CEC of peptides (2009–2011)

Cobalt Oxyhydroxide-prompted Synthesis of Fluorescent Polydopamine Nanoparticles for Glutathione Detection

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