Rapid capillary electrophoretic analysis of human serum proteins: qualitative comparison with high-throughput agarose gel electrophoresis

Clark, Raynell J.; Katzmann, Jerry A.; Wiegert, Elaine; Namyst-Goldberg, Colette; Sanders, L.; Oda, Robert P.; Kyle, Robert A.; Landers, James P.

doi:10.1016/0021-9673(96)00190-2

Cited by 41 publications

(9 citation statements)

References 7 publications

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“…However, by increasing the ionic strength and pH of the run buffer they were able to detect these MCs thus stressing the importance of method validation with known samples prior to use of an instrument in a clinical laboratory. Although other papers describing comparisons with single capillary instruments gave similar results [35][36][37][38][39] it is the multiple capillary instruments that will impact the clinical laboratory. The first description of a multicapillary instrument, Paragon 2000, by Jolliff and Blessum [40] found that CE and AGE had a 96% concordance in discriminating disease states in 240 samples with varying dysproteinemias, such as hypogammaglobulinemia, acute and chronic inflammation, nephrosis, cirrhosis, poly-and monoclonal gammopathies.…”

Section: Serum Proteinssupporting

confidence: 55%

Capillary electrophoresis and the clinical laboratory

et al. 2006

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Over the past 15 years, CE as an analytical tool has shown great promise in replacing many conventional clinical laboratory methods, such as electrophoresis and HPLC. CE's appeal was that it was fast, used very small amounts of sample and reagents, was extremely versatile, and was able to separate large and small analytes, whether neutral or charged. Because of this versatility, numerous methods have been developed for analytes that are of clinical interest. Other than molecular diagnostic and forensic laboratories CE has not been able to make a major impact in the United States. In contrast, in Europe and Japan an increasing number of clinical laboratories are using CE. Now that automated multicapillary instruments are commercially available along with cost-effective test kits, CE may yet be accepted as an instrument that will be routinely used in the clinical laboratories. This review will focus on areas where CE has the potential to have the greatest impact on the clinical laboratory. These include analyses of proteins found in serum and urine, hemoglobin (A1c and variants), carbohydrate-deficient transferrin, forensic and therapeutic drug screening, and molecular diagnostics.

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Section: Serum Proteinssupporting

confidence: 55%

Capillary electrophoresis and the clinical laboratory

et al. 2006

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“…1B) illustrates that, in addition to the second beta peak, some samples show additional alpha peaks. These types of qualitative variations in normal serum have been discussed previously [14]. Serum samples with large monoclonal peaks are clearly identified by all three procedures (e.g., Fig.…”

Section: Comparing the Profiles From Cae And Age With Cementioning

confidence: 94%

Identification of monoclonal proteins in serum: A quantitative comparison of acetate, agarose gel, and capillary electrophoresis

et al. 1997

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A selected group of 308 sera were analyzed by capillary electrophoresis (CE), agarose gel electrophoresis (AGE), and cellulose acetate electrophoresis (CAE) and evaluated for abnormalities that would suggest the presence of a monoclonal protein. The sensitivity (an electrophoretic abnormality in sera that contained a monoclonal protein) and specificity (a normal electrophoretic pattern in sera that did not contain a monoclonal protein) was determined for each electrophoretic procedure. CAE was the most specific procedure and CE was the most sensitive. The increase in sensitivity of CE was primarily due to increased detection of cryoglobulins and free light chains. The quantitation of the gamma region and/or monoclonal antibody peaks by CE was similar to results obtained by AGE. Quantitation of very large monoclonal protein peaks (> 3.0 g/dL) by on-line absorption detection (CE) yielded higher results than quantitation by dye-binding (AGE).

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“…Another advantage of CZE over AGE is the absence of application point artifact, observed in previous studies. 17 Application point artifact is not seen with CZE because the sample is injected into the inlet of the capillary. Suspect tracings in the y region caused by hemolysis of the serum specimen were also eliminated with CZE.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Capillary Zone and Immunosubtraction with Agarose Gel and Immunofixation Electrophoresis for Detecting and Identifying Monoclonal Gammopathies

Litwin

et al. 1999

Am J Clin Pathol

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Rapid capillary electrophoretic analysis of human serum proteins: qualitative comparison with high-throughput agarose gel electrophoresis

Cited by 41 publications

References 7 publications

Capillary electrophoresis and the clinical laboratory

Capillary electrophoresis and the clinical laboratory

Identification of monoclonal proteins in serum: A quantitative comparison of acetate, agarose gel, and capillary electrophoresis

Comparison of Capillary Zone and Immunosubtraction with Agarose Gel and Immunofixation Electrophoresis for Detecting and Identifying Monoclonal Gammopathies

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