Rapid characterization of outer‐membrane proteins in <i>Neisseria lactamica</i> by SELDI–TOF‐MS (surface‐enhanced laser desorption ionization–time‐of‐flight MS) for use in a meningococcal vaccine

Mukhopadhyay, Tarit; Halliwell, Denise; O'Dwyer, Clíona A.; Shamlou, P. Ayazi; Levy, M. Susana; Allison, Nigel; Gorringe, Andrew; Reddin, Karen M.

doi:10.1042/ba20040098

Cited by 24 publications

(2 citation statements)

References 29 publications

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“…Contamination of secreted protein preparations by outer membrane proteins and/or outer membrane vesicles was assessed by the presence or absence of NspA by Western blotting. NspA is an outer membrane protein present in both N. meningitidis and N. lactamica and is a major component of their outer membrane vesicles [30]. No NspA was detected in either the N. meningitidis and N. lactamica secreted protein preparations (data not shown).…”

Section: Resultsmentioning

confidence: 91%

Genome Wide Expression Profiling Reveals Suppression of Host Defence Responses during Colonisation by Neisseria meningitides but not N. lactamica

Wong

Kroll

et al. 2011

PLoS ONE

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Both Neisseria meningitidis and the closely related bacterium Neisseria lactamica colonise human nasopharyngeal mucosal surface, but only N. meningitidis invades the bloodstream to cause potentially life-threatening meningitis and septicaemia. We have hypothesised that the two neisserial species differentially modulate host respiratory epithelial cell gene expression reflecting their disease potential. Confluent monolayers of 16HBE14 human bronchial epithelial cells were exposed to live and/or dead N. meningitidis (including capsule and pili mutants) and N. lactamica, and their transcriptomes were compared using whole genome microarrays. Changes in expression of selected genes were subsequently validated using Q-RT-PCR and ELISAs. Live N. meningitidis and N. lactamica induced genes involved in host energy production processes suggesting that both bacterial species utilise host resources. N. meningitidis infection was associated with down-regulation of host defence genes. N. lactamica, relative to N. meningitidis, initiates up-regulation of proinflammatory genes. Bacterial secreted proteins alone induced some of the changes observed. The results suggest N. meningitidis and N. lactamica differentially regulate host respiratory epithelial cell gene expression through colonisation and/or protein secretion, and that this may contribute to subsequent clinical outcomes associated with these bacteria.

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Section: Resultsmentioning

confidence: 91%

Genome Wide Expression Profiling Reveals Suppression of Host Defence Responses during Colonisation by Neisseria meningitides but not N. lactamica

Wong

Kroll

et al. 2011

PLoS ONE

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“…The cultivation, isolation, and differentiation of colonized species in the nasopharynx of patients and healthy carriers are of great importance. Neisseria's fimbriae (pili) and other surface antigens are associated with attachment, twitching motility, and genetic competence of the bacterium (11). Pathogenic Neisseria strains nutritionally are fastidious and grow at 35 -37°C.…”

Section: Characteristics Of Neisseria Genusmentioning

confidence: 99%

Characteristics of Neisseria Species Colonized in the Human’s Nasopharynx

Tavana

Ataee

Jonaidi

et al. 2020

Jundishapur J Microbiol

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Context: Neisseria meningitidis is the causative agent of a life-threatening infection with high mortality and morbidity worldwide. The most common types of this bacterium are serogroups A, B, C, W135, X, and Y. Although in some countries, such as Iran, the meningococcal meningitis has been well monitored and controlled by the use of divalent and quadrivalent vaccines, other fatal infections caused by these bacteria are still an important threat. For the above reason, this review focused on the differences of Neisseria characteristics, particularly in capsular composition, pathogenic and commensal stages to a better understanding of how to manage Neisseria infections. Evidence Acquisition: In this review, PubMed, EMBASE, ScienceDirect, Scopus, and Google Scholar were searched for English-language publications on pathogenic or commensal strains of Neisseria, meningococcal disease, Neisseria biology, genetic diversity, molecular typing, serogroups, diagnostic, and epidemiology around the world up to July 2019. All articles and academic reports in the defined area of this research were considered too. The data were extracted and descriptively discussed. Results: We included 85 studies in the survey. The data analysis revealed that the distribution of meningococcal serogroups was different regionally. For example, the serogroups C and W-135 accounted for Africa and Latin America regions, serogroup B in the European countries, and rarely in the Western Pacific, and serogroups A and C were dominant in Asian countries. Although data set for laboratory-based diagnosis of N. meningitidis are available for all countries, only 30% of the countries rely on reference laboratories for serogroup determination, and more than half of the countries lack the ability of surveillance system. Nevertheless, molecular detection procedure is also available for all countries. The use of the meningococcal vaccine is a variable country by country, but most countries have applied the meningococcal vaccine, either divalent or quadrivalent, for the protection of high-risk groups. Conclusions: Owing to the geographical distribution of N. meningitidis serogroups in circulating, each country has to monitor for changes in serogroups diversity and its control management. Furthermore, laboratories should scale up the epidemiology and disease burden. It should be mentioned that quadrivalent meningococcal vaccines reduce the meningococcal disease burden sharply.

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Bioprocess monitoring and computer control: Key roots of the current PAT initiative

Junker

Wang

2006

Biotech & Bioengineering

115

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This review article has been written for the journal, Biotechnology and Bioengineering, to commemorate the 70th birthday of Daniel I.C. Wang, who served as doctoral thesis advisor to each of the co-authors, but a decade apart. Key roots of the current PAT initiative in bioprocess monitoring and control are described, focusing on the impact of Danny Wang's research as a professor at MIT. The history of computer control and monitoring in biochemical processing has been used to identify the areas that have already benefited and those that are most likely to benefit in the future from PAT applications. Past applications have included the use of indirect estimation methods for cell density, expansion of on-line/at-line and on-line/in situ measurement techniques, and development of models and expert systems for control and optimization. Future applications are likely to encompass additional novel measurement technologies, measurements for multi-scale and disposable bioreactors, real time batch release, and more efficient data utilization to achieve process validation and continuous improvement goals. Dan Wang's substantial contributions in this arena have been one key factor in steering the PAT initiative towards realistic and attainable industrial applications.

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Rapid characterization of outer‐membrane proteins in Neisseria lactamica by SELDI–TOF‐MS (surface‐enhanced laser desorption ionization–time‐of‐flight MS) for use in a meningococcal vaccine

Cited by 24 publications

References 29 publications

Genome Wide Expression Profiling Reveals Suppression of Host Defence Responses during Colonisation by Neisseria meningitides but not N. lactamica

Genome Wide Expression Profiling Reveals Suppression of Host Defence Responses during Colonisation by Neisseria meningitides but not N. lactamica

Characteristics of Neisseria Species Colonized in the Human’s Nasopharynx

Bioprocess monitoring and computer control: Key roots of the current PAT initiative

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