Rapid characterization of the absorbed chemical constituents of Tangzhiqing formula following oral administration using UHPLC–Q‐TOF‐MS

Wu, Xiaolei; Wu, Mingjiang; Xin-ze, Chen; Zhang, Hongmin; Ding, Lei; Fang, Tian; Fu, Xiaomeng; Qiu, Feng; Zhang, Deqin

doi:10.1002/jssc.201700777

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…Diterpenoid quinines are the major components in S. miltiorrhiza. In addition, more sensitive fragmentation information was observed in the positive ion mode, as they are easy to capture a proton to form the molecular ions 14 . The neutral loss of H 2 O and CO was supposed to be generated from the cleavage of the carbonyl linkage on the C ring.…”

Section: Resultsmentioning

confidence: 99%

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Characterization of chemical components in the Guanxinning injection by liquid chromatography–mass spectrometry

Cui

Zheng

et al. 2020

J Mass Spectrom

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Guanxinning injection (GXNI) is widely used in the treatments of cerebral thrombosis, cerebral hemorrhage, sequela, coronary disease, stenocardia, arrhythmia, and so on. For the herbal injections, more components should be characterized and quantified as much as possible to guarantee the drug safety. However, large numbers of the chemical constituents in the GXNI still remain unknown. In this study, ultrahigh performance liquid chromatography‐Q Exactive hybrid quadrupole‐orbitrap high‐resolution accurate mass spectrometry (UHPLC‐Q Orbitrap HRMS), in combination of nuclear magnetic resonance (NMR), was used to identify the components in GXNI, which led to the identification of 194 compounds. With the aid of solvent partition, more phthalides, diterpenoid quinines, and salvianolic acids were tentatively identified, and minor compounds with the other structural types were also detected. The structural diversity of phthalides and diterpenoid quinones were revealed by the structural network, and six phthalides and 13 diterpenoid quinones were further detected in GXNI with the help of the characteristic fragmentation pattern and structural network. In addition, NMR also revealed the presence of a series of primary metabolites in the GXNI, which could be used as a complimentary approach for the rapid identification of the chemical components in the traditional Chinese medicines (TCM). However, the unknown NMR signals of GXNI needed to be further identified to guarantee the drug safety.

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Section: Resultsmentioning

confidence: 99%

“…In addition, more sensitive fragmentation information was observed in the positive ion mode, as they are easy to capture a proton to form the molecular ions. 14…”

Section: Diterpenoid Quinonesmentioning

confidence: 99%

Characterization of chemical components in the Guanxinning injection by liquid chromatography–mass spectrometry

Cui

Zheng

et al. 2020

J Mass Spectrom

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“…Based on chemical and metabolic analyses of TZQ disposition, 86 components and 48 metabolites were detected in rat plasma, urine, and feces. The primary components absorbed are flavonoids and alkaloids, while the primary metabolites are glucuronides and sulfates ( Wu et al, 2018b ).…”

Section: Introductionmentioning

confidence: 99%

Integrated pyroptosis measurement and metabolomics to elucidate the effect and mechanism of tangzhiqing on atherosclerosis

Chen

Zhou

et al. 2022

Front. Physiol.

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Tangzhiqing formula (TZQ) is a traditional Chinese medicine prescribed to treat glucose and lipid metabolism disorders. A significant effect of TZQ on diabetes and hyperlipidemia has been demonstrated, but its effect on atherosclerosis (AS) remains unknown. This study combines pyroptosis with metabolomics to elucidate the effect and mechanism of TZQ on AS. A model of AS was developed using ApoE−/− mice fed a high-fat diet for 8 weeks. After 6 weeks of atorvastatin (Ator) or TZQ treatment, aortic lumen diameter, aortic lesion size, serum lipid profile, cytokines, and Nod-like receptor protein 3 (NLRP3) inflammasome-mediated pyroptosis were analyzed. Serum metabolomics profiles were obtained to examine the effect of TZQ on AS and the correlation between pyroptosis and metabolites was further analyzed. As a result, TZQ significantly reduced the diameter of the common carotid artery during diastole and the blood flow velocity in the aorta during systole; reduced blood lipid levels, arterial vascular plaques, and the release of inflammatory cytokines; and inhibited the NLRP3 inflammasome-mediated pyroptosis. According to metabolomics profiling, TZQ is engaged in the treatment of AS via altering arachidonic acid metabolism, glycerophospholipid metabolism, steroid hormone production, and unsaturated fatty acid biosynthesis. The cytochrome P450 enzyme family and cyclooxygenase 2 (COX-2) are two major metabolic enzymes associated with pyroptosis.

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“…An in vitro experiment demonstrated that 2,3,4,6-tetra-O-galloyl-D-glucose, 1,2,3,4-tetra-O-galloyl-D-glucose, 1,2,3,4,6-penta-O-galloyl-d-glucose, quercetin-3-O-β-D-glucuronide and quercetin-3-O-β-D-glucoside were the primary biological active components of SYT due to the inhibitory effects on maltase, invertase, and lipase ( Tao et al, 2013 ). After oral administration of SYT, 86 components were identified in rat plasma, urine, and feces, including alkaloids, flavonoids, phenolic acid, diterpenoid quinones, monoterpenoids, and metabolites of glucuronidation and sulfation, which were found to be absorbed components ( Wu et al, 2018 ). Nuciferine and paeoniflorin were suggested as the quality markers of SYT ( Li et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Sanye Tablet Ameliorates Insulin Resistance and Dysregulated Lipid Metabolism in High-Fat Diet-Induced Obese Mice

Yao

Huang

et al. 2021

Front. Pharmacol.

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Sanye Tablet (SYT) is a patent prescription widely used in treating T2D and pre-diabetes, especially T2D comorbid with hypertriglyceridemia, for many years in China. However, the underlying mechanism that accounts for the anti-diabetic potential of SYT by regulating lipid-related intermediates remains to be elucidated. This study aimed to investigate the mechanism of SYT on lipid metabolism and insulin sensitivity in high-fat diet (HFD)-induced obese mice by means of combining lipidomics and proteomics. The obese mice models were developed via HFD feeding for 20 consecutive weeks. Mice in the treatment group were given metformin and SYT respectively, and the effects of SYT on body weight, blood glucose, insulin sensitivity, fat accumulation in the organs, and pathological changes in the liver were monitored. Lipid metabolism was examined by lipidomics. Further determination of signaling pathways was detected by proteomics. The biological contributions of the compounds detected in SYT’s chemical fingerprint were predicted by network pharmacology. SYT treatment reduced body weight, inhibited viscera and hepatic steatosis lipid accumulation, and prevented insulin resistance. Furthermore, it was found that circulatory inflammatory cytokines were reduced by SYT treatment. In addition, lipidomics analysis indicated that SYT targets lipid intermediates, including diacylglycerol (DAG) and Ceramide (Cer). Mechanistically, SYT positively affected these lipid intermediates by suppressing liver lipogenesis via downregulation of SREBP1/ACC and the JAK/STAT signaling pathway. Our results predicted that astragalin and rosmarinic acid might regulate the JAK-STAT pathway by targeting PIM2 and STAT1, respectively, while paeoniflorin and rosmarinic acid were likely to regulate inflammatory responses by targeting TNFα, IL-6, and IL-4 during T2D. Overall, our study provides supportive evidence for the mechanism of SYT’s therapeutic effect on dysregulated lipid metabolism in diabesity.

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Rapid characterization of the absorbed chemical constituents of Tangzhiqing formula following oral administration using UHPLC–Q‐TOF‐MS

Cited by 10 publications

References 28 publications

Characterization of chemical components in the Guanxinning injection by liquid chromatography–mass spectrometry

Characterization of chemical components in the Guanxinning injection by liquid chromatography–mass spectrometry

Integrated pyroptosis measurement and metabolomics to elucidate the effect and mechanism of tangzhiqing on atherosclerosis

Sanye Tablet Ameliorates Insulin Resistance and Dysregulated Lipid Metabolism in High-Fat Diet-Induced Obese Mice

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