Rapid characterization of the constituents in Jigucao capsule using ultra high performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry

Abstract

Jigucao capsule is a well‐known Chinese patent medicine for the treatment of acute and chronic hepatitis and cholecystitis. The chemical components of Jigucao capsule were not clear resulting from the paucity of relevant studies, which hindered the research of the pharmacological mechanism, the comprehensive development, and utilization of Jigucao capsule in clinical studies. By establishing a high‐throughput ultra‐performance liquid chromatography quadrupole time of flight mass spectrometry in combination with intelligent UNIFI software data processing platform to automatically characterize and identify the chemical profile of Jigucao capsule, 144 compounds were determined rapidly, including 34 terpenoids, 25 flavonoids, 22 steroids, 21 phenylpropanoids, 10 glycosides, six alkaloids, 13 organic acids, and other 13 components. These compounds may be the active components of Jigucao capsule. In this study, a rapid and robust method for comprehensively analyzing the chemical composition of Jigucao capsule was described and established for the first time. The results will provide a reference for the quality control of Jigucao capsule and the establishment of a higher quality standard, as well as for the pharmacodynamic material basis research.

The chemical profile ofFubai Chrysanthemum(Fubaiju) and its mechanism in preventing cataract based on ultrahigh‐performance liquid chromatography coupled with mass spectrometry and network pharmacology

“…The raw UHPLC‐Q‐TOF‐MS data in positive and negative mode were analyzed by using UNIFI software and identified by comparison with typical fragmentation pathways of known substances [11]. Eventually, 39 flavonoids and phenolic acids were identified including 28 flavonoids, 10 phenolic acids, and umbelliferone.…”

Metabolomics Analysis Coupled With UPLC/MS on Therapeutic Effect of Jigucao Capsule Against Dampness-Heat Jaundice Syndrome

“…JGCC is a good medicine for the treatment of hepatitis and cholecystitis (Zhao et al, 2009) and contains a large number of chemical components such as alkaloids, flavonoids, terpenes and organic acids. In the early stage, we have systematically studied the chemical components of JGCC, and 144 compounds have been identified (Li et al, 2021a). JGCC used in this study is a commercial drug.…”

Rapid characterization of chemical constituents in Naoling Pian by LC‐MS combined with data processing techniques

“…Due to the high separation ability of the liquid phase, and the high sensitivity and selectivity of MS, LC-MS has certain advantages in analyzing the complex and diverse chemical components of TCM [18], which is becoming increasingly popular among scholars as one of the component analysis techniques [19][20][21][22]. To further clarify the composition of NLP, an MS E scanning mode was employed in this study, which can simultaneously acquire and obtain precursor ions and product ion fragments, using the feature of simultaneous scanning of high and low collision energies can significantly improve the efficiency of sample collection and analysis [23].…”