Rapid classification of bacterial strains by SDS-polyacrylamide gel electrophoresis: population dynamics of the dominant dispersed phase bacteria of activated sludge

Hantula, Jarkko; Kurki, A.; Vuoriranta, P.; Bamford, Dennis H.

doi:10.1007/bf00180587

Cited by 20 publications

(14 citation statements)

References 17 publications

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“…Although SDS-PAGE of bacterial whole-cell protein extracts is shown to be very sensitive to taxonomic differences its use is still limited in some bacterial species (20,21,26,38). showed high similarity with the strain FO-033 of B. pumilus isolated from spacecraft (53).…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)

Figueiredo¹,

Gomes²,

Guimarães³

et al. 2009

Braz. J. Microbiol.

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Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some highaccumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing, Bacillus subitilis and B. pumilus were the most frequenty species (15 and 12 isolates, respectively) followed by B. licheniformes (7 isolates), B. cereus (5 isolates) and B. amiloliquefascens (3 isolates). According to present results, SDS-PAGE technique could be used as a fast and cheap first tool for identifying interspecific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies.

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Section: Discussionmentioning

confidence: 99%

Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)

Figueiredo¹,

Gomes²,

Guimarães³

et al. 2009

Braz. J. Microbiol.

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“…The changes in flocculation caused by chemical agents could in principle take place either via regulating gene expression or by physically or chemically modifying floc formation. However, the capability of pure culture flocculation is c o m m o n among bacteria isolated from activated sludge (Kurki et al 1989;Hantula et al 1991). As E D T A did not disperse the flocs, and as the addition of CaC12 did not restore flocculation at low citrate concentration, the structures linking cells to each other are not mediated by calcium ions as, for example, in m a n y viral capsid protein interactions (see Olkkonen et al 1991).…”

Section: Discussionmentioning

confidence: 99%

The efficiency of the protein-dependent flocculation of Flavobacterium sp. is sensitive to the composition of growth medium

Hantula

Bamford

1991

Appl Microbiol Biotechnol

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The pure culture flocculation of Flavobacterium sp. strain 304 was shown to be limited to early stationary phase cells. The effects of proteolytic, cellulolytic and nucleolytic enzymes as well a agents such as divalent ions, citrate, malate, EDTA, poly-L-lysine, and NaN3 on flocculation was studied. The results indicate that the flocculation of strain 304 is protein mediated, and affected by composition of the growth medium. The primary interaction between the cells is not ionic or divalent cation dependent, but probably hydrophobic in nature. In addition, the role of single-species flocculation in activated sludge is discussed.

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“…If the interrelation of phage resistance and flocculation deficiency turns out to be a common phenomenon, phage infections of activated sludge bacteria might have an effect on the settleability of a single species. As the microbial diversity of the activated sludge is high (Hantula et al 1991a) we do not assume that a major malfunction of the plant would be caused by phage infection. However, the minor variation in settleability could well be partly caused by phages altering the surface properties of the sludge bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…The most important of these are bacteria, which are the primary users of incoming organic material, and which are predated by protozoa. The total number of bacterial species is estimated to be in the order of thousands (Hantula et al 1991a), although the proportion of active species is not known.…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage resistance and flocculation deficiency of Flavobacterium sp. are phenotypically interrelated

Hantula

Bamford

1991

Appl Microbiol Biotechnol

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An analysis of Flavobacterium sp. strain 304 variants selected for flocculation deficiency and phage resistance has been carried out. The results show that flocculation is efficiently hindered by overexpressed protein in the outer membrane or carbohydrate on the cell surface. The floc-forming protein is suggested to be a minor component of the cell surface. The 12 flocculation-deficient and five phage-insensitive variants obtained were grouped into ten classes based on nine characteristics. In addition, bacteriophage d~304 resistance and the pure culture flocculation deficiency of this strain are interrelated.

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Rapid classification of bacterial strains by SDS-polyacrylamide gel electrophoresis: population dynamics of the dominant dispersed phase bacteria of activated sludge

Cited by 20 publications

References 17 publications

Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)

Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)

The efficiency of the protein-dependent flocculation of Flavobacterium sp. is sensitive to the composition of growth medium

Bacteriophage resistance and flocculation deficiency of Flavobacterium sp. are phenotypically interrelated

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