Rapid collagen peptide mass fingerprinting as a tool to authenticate Pleuronectiformes in the food industry

Dierickx, Katrien; Presslee, Samantha; Harvey, Virginia L.

doi:10.1016/j.foodcont.2023.109680

Cited by 6 publications

(4 citation statements)

References 52 publications

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“…Applying these biomarkers to additional Mediterranean archaeological grouper remains could generate critical insights into how species distributions and abundances have changed over time. Species‐specific peptide biomarkers for Mediterranean groupers have the potential to aid in identifying species in present‐day fish markets (Dierickx et al., 2023 ; Mazzeo et al., 2008 ). As grouper meat is highly organoleptic, it is not uncommon for grouper in Mediterranean fish markets to be falsely labelled and substituted for cheaper fishes (Asensio et al., 2008 ; Cutarelli et al., 2014 ; Giusti et al., 2022 ; Trotta et al., 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Grouping groupers in the Mediterranean: Ecological baselines revealed by ancient proteins

Winter,

de Kock,

Mackie

et al. 2023

Ecology and Evolution

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Marine historical ecology provides a means to establish baselines to inform current fisheries management. Groupers (Epinephelidae) are key species for fisheries in the Mediterranean, which have been heavily overfished. Species abundance and distribution prior to the 20th century in the Mediterranean remains poorly known. To reconstruct the past biogeography of Mediterranean groupers, we investigated whether Zooarchaeology by Mass Spectrometry (ZooMS) can be used for identifying intra‐genus grouper bones to species level. We discovered 22 novel, species‐specific ZooMS biomarkers for groupers. Applying these biomarkers to Kinet Höyük, a Mediterranean archaeological site, demonstrated 4000 years of regional Epinephelus aeneus dominance and resiliency through millennia of fishing pressures, habitat degradation and climatic changes. Combining ZooMS identifications with catch size reconstructions revealed the Epinephelus aeneus capacity for growing 30 cm larger than hitherto documented, revising the maximum Total Length from 120 to 150 cm. Our results provide ecological baselines for a key Mediterranean fishery which could be leveraged to define and assess conservation targets.

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Section: Discussionmentioning

confidence: 99%

Grouping groupers in the Mediterranean: Ecological baselines revealed by ancient proteins

Winter,

de Kock,

Mackie

et al. 2023

Ecology and Evolution

Self Cite

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show abstract

“…Applying these biomarkers to additional Mediterranean archaeological grouper remains could generate critical insights into how species distributions and abundances have changed over time. Species-specific peptide biomarkers for Mediterranean groupers have the potential to aid in identifying species in present-day fish markets (Dierickx et al, 2023;Mazzeo et al, 2008). As grouper meat is highly organoleptic, it is not uncommon for grouper in Mediterranean fish markets to be falsely labelled and substituted for cheaper fishes (Asensio et al, 2008;Cutarelli et al, 2014;Giusti et al, 2022;Trotta et al, 2005).…”

Section: Col1 Utility For Mediterranean Grouper Phylogeny and Zooms I...mentioning

confidence: 99%

Grouping groupers in the Mediterranean: ecological baselines revealed by ancient proteins

Winter,

Kock,

Mackie

et al. 2023

Preprint

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Marine historical ecology provides a means to establish baselines to inform current fisheries management. Groupers (Epinephelidae) are key species for fisheries in the Mediterranean which have been heavily overfished. Species abundance and distribution prior to the 20th century in the Mediterranean remains poorly known. To reconstruct the past biogeography of Mediterranean groupers, we investigated if Zooarchaeology by Mass Spectrometry (ZooMS) can be used for identifying intra-genus grouper bones to species level. We discovered 22 novel, species-specific ZooMS biomarkers for groupers. Applying these biomarkers to a northeastern Mediterranean archaeological site demonstrated 4000 years of regional Epinephelus aeneus dominance and resiliency through millennia of fishing pressures, habitat degradation, and climatic changes. Combining ZooMS identifications with catch size reconstructions revealed the Epinephelus aeneus capacity for growing 30 cm larger than hitherto documented. Our results provide ecological baselines for a key Mediterranean fishery which could be leveraged to define and assess conservation targets.

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“…Spectroscopic techniques coupled with chemometrics and molecular methods are the most widely used for fish authenticity testing. As for proteinbased techniques, these include proteomics based on mass spectrometry [7][8][9][10][11][12][13], twodimensional electrophoresis with subsequent analysis with mass spectrometry [14], isoelectric focusing [15,16], enzyme-linked immunosorbent assay (ELISA) [17][18][19][20], and capillary electrophoresis [21]. The methods developed for the identification of individual species are time-consuming and less successful in processed foods since the specific morphological traits are transformed throughout the processing.…”

Section: Introductionmentioning

confidence: 99%

Molecular Rapid Test for Identification of Tuna Species

Gkini,

Christopoulos,

Conides

et al. 2024

Biosensors

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Tuna is an excellent food product, relatively low in calories, that is recommended for a balanced diet. The continuously increasing demand, especially for bluefin-tuna-based food preparations, and its relatively high market price make adulteration by intentionally mixing with other lower-priced tunas more prospective. The development of rapid methods to detect tuna adulteration is a great challenge in food analytical science. We have thus developed a simple, fast, and low-cost molecular rapid test for the visual detection of tuna adulteration. It is the first sensor developed for tuna authenticity testing. The three species studied were Thunnus thynnus (BFT), Thunnus albacares, and Katsuwonus pelamis. DNA was isolated from fresh and heat-treated cooked fish samples followed by PCR. The PCR products were hybridized (10 min) to specific probes and applied to the rapid sensing device. The signal was observed visually in 10–15 min using gold nanoparticle reporters. The method was evaluated employing binary mixtures of PCR products from fresh tissues and mixtures of DNA isolates from heat-treated tissues (canned products) at adulteration percentages of 1–100%. The results showed that the method was reproducible and specific for each tuna species. As low as 1% of tuna adulteration was detected with the naked eye.

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Rapid collagen peptide mass fingerprinting as a tool to authenticate Pleuronectiformes in the food industry

Cited by 6 publications

References 52 publications

Grouping groupers in the Mediterranean: Ecological baselines revealed by ancient proteins

Grouping groupers in the Mediterranean: Ecological baselines revealed by ancient proteins

Grouping groupers in the Mediterranean: ecological baselines revealed by ancient proteins

Molecular Rapid Test for Identification of Tuna Species

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