Rapid colorimetric detection of H2O2 in living cells and its upstream series of molecules based on oxidase-like activity of CoMnO3 nanofibers

Calcium Fluoride/Manganese Dioxide Nanocomposite with Dual Enzyme-like Activities for Uric Acid Sensing: A Comparative Study of Enzyme and Nonenzyme Methods

“…The thiol-blocking reagent N-ethylmaleimide (NEM) was introduced to remove the interference of sulfhydryl material in human serum. 40 The obtained UA levels by both methods were in good agreement with the biochemical analysis, providing good accuracy and precision with small relative errors and relative standard deviations (RSD, n = 3). UA sensing in spiked samples was also carried out to further confirm the viability of both methods in biological matrices.…”

“…Multiple human serum samples were utilized as real samples for the determination of UA in practical applications (Table ). The thiol-blocking reagent N -ethylmaleimide (NEM) was introduced to remove the interference of sulfhydryl material in human serum . The obtained UA levels by both methods were in good agreement with the biochemical analysis, providing good accuracy and precision with small relative errors and relative standard deviations (RSD, n = 3).…”

“…Further work showed that they had no effect on the system at respective doses of 10, 5, and 5 μM, respectively (Figure S19). Fortunately, the concentrations of these three substances in serum were far lower than those of UA, indicating that none of these substances interfered with the serum sample assay and the developed assay had good selectivity.…”

Ratiometric colorimetric detection of nitrite using CoMnO3 nanofibers as an oxidase-like enzyme to induce diazotization reaction

“…CoMnO 3 nanofibers were synthesized according to the previous literature 27 with a slight modification. KMnO 4 (0.75 g) and MnAc 2 ·4H 2 O (0.45 mg) were dissolved in 30 mL ultrapure water, and the mixture was stirred at 25 °C for 20 min to dissolve completely.…”

Quantification of Neuronal Cell-Released Hydrogen Peroxide Using 3D Mesoporous Copper-Enriched Prussian Blue Microcubes Nanozymes: A Colorimetric Approach in Real Time and Anticancer Effect

“…Therefore, the ongoing challenge remains the development of real-time, selective, and sensitive detection of H 2 O 2 released by living cells. On the other hand, the colorimetric assay for H 2 O 2 has gained widespread recognition as a potent and versatile approach. , Its capacity for rapid and straightforward visual detection offers significant potential for enhancing point-of-care diagnostics. Despite their effectiveness and specificity, horseradish peroxidase (HRP) has conventionally been used in H 2 O 2 colorimetric sensors.…”