Rapid colorimetric detection of p53 protein function using DNA-gold nanoconjugates with applications for drug discovery and cancer diagnostics

Assah, Enock; Goh, Walter L.; Zheng, Xin Ting; Lim, Ting Xiang; Li, Jun; Lane, David P.; Ghadessy, Farid J.; Tan, Yen Nee

doi:10.1016/j.colsurfb.2018.05.007

Cited by 36 publications

(27 citation statements)

References 43 publications

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“…In 2011, Lee and co-workers used AuNP-based colorimetric assays for ultra-selective detection of cancer mutation points (BRCA1) by base pairing-induced AuNP aggregation [66]. This system is also used for detection of the mutation of the epidermal growth factor receptor gene (EGFR) [67], the Kirsten rat sarcoma viral oncogene homologue gene (KRAS) [68] or p53 [69]. In the AuNP-based colorimetric assay, the signal can be amplified through isothermal [68] or polymerase chain reaction [70].…”

Section: Colorimetric Responsesmentioning

confidence: 99%

The Basic Properties of Gold Nanoparticles and their Applications in Tumor Diagnosis and Treatment

Bai

Wang

Song

et al. 2020

IJMS

278

156

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Gold nanoparticles (AuNPs) have been widely studied and applied in the field of tumor diagnosis and treatment because of their special fundamental properties. In order to make AuNPs more suitable for tumor diagnosis and treatment, their natural properties and the interrelationships between these properties should be systematically and profoundly understood. The natural properties of AuNPs were discussed from two aspects: physical and chemical. Among the physical properties of AuNPs, localized surface plasmon resonance (LSPR), radioactivity and high X-ray absorption coefficient are widely used in the diagnosis and treatment of tumors. As an advantage over many other nanoparticles in chemicals, AuNPs can form stable chemical bonds with S-and N-containing groups. This allows AuNPs to attach to a wide variety of organic ligands or polymers with a specific function. These surface modifications endow AuNPs with outstanding biocompatibility, targeting and drug delivery capabilities. In this review, we systematically summarized the physicochemical properties of AuNPs and their intrinsic relationships. Then the latest research advancements and the developments of basic research and clinical trials using these properties are summarized. Further, the difficulties to be overcome and possible solutions in the process from basic laboratory research to clinical application are discussed. Finally, the possibility of applying the results to clinical trials was estimated. We hope to provide a reference for peer researchers to better utilize the excellent physicochemical properties of gold nanoparticles in oncotherapy.

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Section: Colorimetric Responsesmentioning

confidence: 99%

The Basic Properties of Gold Nanoparticles and their Applications in Tumor Diagnosis and Treatment

Bai

Wang

Song

et al. 2020

IJMS

278

156

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“…Plasmonic nanoparticles (NPs) such as gold and silver NPs, have unprecedented ability to localize and enhance their surrounding electric eld and conne it into subwavelength volumes due to the excitation of their surface plasmon resonance (SPR) by the incident electromagnetic eld, leading to several applications from structural color printing, 8,9 energy storage, 10 and catalysis [11][12][13][14] to metal NPs-based biosensors [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] development. More recently, these localised SPR effects have been exploited to enhance the optoelectronic properties of functional materials, such as uorophores and photosensitizers, leading to a new eld of studying metal-enhanced uorescence (MEF) [30][31][32][33][34][35][36][37][38][39][40][41] and singlet oxygen generation (ME-SOG).…”

Section: Introductionmentioning

confidence: 99%

Metal-enhancement study of dual functional photosensitizers with aggregation-induced emission and singlet oxygen generation

Yaraki

Rezaei

et al. 2020

Nanoscale Adv.

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“…Recent studies have proven that nanoparticles can be used as colorimetric probes to develop versatile biosensors (also known as nanobiosensors) for virus detection [115][116][117][118][119][120][121]. This is largely designed based on the unique optical properties of the particular nanoparticles that can cause obvious color changes for naked eye detection or quantitative measurement by simple optical detector.…”

Section: Nanoparticles For Virus Detectionsmentioning

confidence: 99%

Colorimetric biosensors for point-of-care virus detections

Zhao

Wong

Zheng

et al. 2020

Materials Science for Energy Technologies

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102

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a b s t r a c tColorimetric biosensors can be used to detect a particular analyte through color changes easily by naked eyes or simple portable optical detectors for quantitative measurement. Thus, it is highly attractive for point-of-care detections of harmful viruses to prevent potential pandemic outbreak, as antiviral medication must be administered in a timely fashion. This review paper summaries existing and emerging techniques that can be employed to detect viruses through colorimetric assay design with detailed discussion of their sensing principles, performances as well as pros and cons, with an aim to provide guideline on the selection of suitable colorimetric biosensors for detecting different species of viruses. Among the colorimetric methods for virus detections, loop-mediated isothermal amplification (LAMP) method is more favourable for its faster detection, high efficiency, cheaper cost, and more reliable with high reproducible assay results. Nanoparticle-based colorimetric biosensors, on the other hand, are most suitable to be fabricated into lateral flow or lab-on-a-chip devices, and can be coupled with LAMP or portable PCR systems for highly sensitive on-site detection of viruses, which is very critical for early diagnosis of virus infections and to prevent outbreak in a swift and controlled manner.

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Rapid colorimetric detection of p53 protein function using DNA-gold nanoconjugates with applications for drug discovery and cancer diagnostics

Cited by 36 publications

References 43 publications

The Basic Properties of Gold Nanoparticles and their Applications in Tumor Diagnosis and Treatment

The Basic Properties of Gold Nanoparticles and their Applications in Tumor Diagnosis and Treatment

Metal-enhancement study of dual functional photosensitizers with aggregation-induced emission and singlet oxygen generation

Colorimetric biosensors for point-of-care virus detections

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