Rapid Colorimetric Micro-method for Estimating Glucose in Blood and C.S.F. Using Glucose Oxidase

Middleton, John; Griffiths, William J.

doi:10.1136/bmj.2.5060.1525

Cited by 97 publications

(24 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, experiments were carried out with albumin solution on the serosal side and bilirubin, dissolved in taurocholate, on the mucosal side. The sacs were incubated with constant shaking for 1 hour at 370 C in 95%o oxygen and 5% carbon dioxide, after which serosal and mucosal fluid was analyzed for bilirubin (28) and glucose (33) concentrations.…”

Section: Methodsmentioning

confidence: 99%

Intestinal Absorption of Bile Pigments. I. The Enterohepatic Circulation of Bilirubin in the Rat*

Lester¹,

Schmid²

1963

J. Clin. Invest.

132

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Intestinal Absorption of Bile Pigments. I. The Enterohepatic Circulation of Bilirubin in the Rat*

Lester¹,

Schmid²

1963

J. Clin. Invest.

132

View full text Add to dashboard Cite

“…A few observations were also made by Bidwell's (1953) method and were generally in agreement. Blood glucose concentration was determined by Middleton & Griffiths' (1957) method. Table 1 gives the response of each subject to each drug, as a percentage of the pre-infusion potency.…”

Section: Methodsmentioning

confidence: 99%

Increase in antihaemophilic globulin activity following infusion of adrenaline

Ingram¹

1961

The Journal of Physiology

154

View full text Add to dashboard Cite

Christie, Graham-Stewart & Ingram (1960) described an abdomino-perineal excision of the rectum in a haemophilic of moderate severity who received animal antihaemophilic globulin (AHG) to cover the operation and post-operative period. Responses to this material were measured by assaying the AHG activity in the patient's plasma before and after each dose; and on two occasions the responses were strikingly high. These peaks corresponded to episodes of acute haemorrhage requiring immediate blood transfusion; and there was also an intermediate peak coinciding with a lesser haemorrhage insufficient to necessitate transfusion. It was not clear whether the high responses correlated with the severity of the bleeding or with blood transfusion; but in another haemophilic an unexpectedly high AHG level was associated with haematemesis while the patient was under observation only. It seemed therefore that haemorrhage was the correlated factor; and in considering a possible mechanism, the extensive previous work on the acceleration of blood coagulation after the administration of adrenaline was recalled: this was reviewed by Forwell (1955), and the problem was reexamined by Forwell & Ingram (1957), who thought at that time that the effect depended on an increase in the activity of Factor V. A possible explanation of the observations in the haemophilics was that haemorrhage had led to a release of adrenaline (Greever & Watts, 1959), and that this had in turn brought about an increase in the activity of AHG in the patients' plasmas. Forwell & Ingram (1957) had not examined AHG in their experimental subjects who had received adrenaline; it therefore seemed of interest to extend the earlier work to include AHG. The results of these experiments are now presented. METHODS The healthy experimental volunteers taking part in the present investigation were senior male medical students. The haemophilic subjects were adult patients, also of course, males, and in good health apart from their haemophilia. Neither they nor the healthy subjects were fasted before testing.

show abstract

“…Erythrocyte volumes were expressed as haematocrit values, which were determined in duplicate on a Hawksley micro-haematocrit centrifuge. D-Glucose was measured by the method of Middleton & Griffiths (1957). Glucose 6-phosphate and fructose 6-phosphate were determined by the method of Hohorst (1965).…”

Section: Methodsmentioning

confidence: 99%

Anaerobic Glycolysis in Normal Human Erythrocytes Incubated in Vitro with Sodium Salicylate

Worathumrong

Grimes

1975

Clinical Science

View full text Add to dashboard Cite

1. Some effects of sodium salicylate upon anaerobic glycolysis have been studied in normal human erythrocytes incubated for up to 6 h at 37 degrees C in autologous sera. 2. Both glucose consumption and lactate production were stimulated by concentrations of salicylate up to 60 mmol/l but at the highest concentration used (90 mmol/l) an initial stimulus was followed by inhibition of glycolysis. 3. Losses occurred of adenosine 5'-triphosphate (ATP), adenosine 5'-diphosphate (ADP) and adenosine 5'-phosphate(AMP)at higher concentrations of salicylate and there was a concomitant increase of inorganic phosphate. 4. Other phosphate esters underwent concentration changes at higher concentrations of salicylate that reflected inadequate concentrations of ATP for glycolysis. 5. The rates of sodium efflux from, and potassium influx into, erythrocytes were unaffected by the presence of salicylate at concentrations sufficient to stimulate glycolysis.

show abstract

Rapid Colorimetric Micro-method for Estimating Glucose in Blood and C.S.F. Using Glucose Oxidase

Cited by 97 publications

References 5 publications

Intestinal Absorption of Bile Pigments. I. The Enterohepatic Circulation of Bilirubin in the Rat*

Intestinal Absorption of Bile Pigments. I. The Enterohepatic Circulation of Bilirubin in the Rat*

Increase in antihaemophilic globulin activity following infusion of adrenaline

Anaerobic Glycolysis in Normal Human Erythrocytes Incubated in Vitro with Sodium Salicylate

Contact Info

Product

Resources

About