Rapid colorimetric sensing platform for the detection of Listeria monocytogenes foodborne pathogen

Alhogail, Sahar; Suaifan, Ghadeer A. R. Y.; Zourob, Mohammed

doi:10.1016/j.bios.2016.07.043

Cited by 117 publications

(50 citation statements)

References 32 publications

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“…innocua and promiscuity towards S. aureus, our data in the nanobrush system shows that the output signal is relatively low when compared to the capture of L. monocytogenes. 66 are rapid (including loop-mediated isothermal amplification or LAMP), with total analysis time of less than one hour, but with poor LOD values near 10 2 CFU-mL -1 . The LOD can be improved considerably with sample pretreatment 67 , but this adds to the analysis time, which is not desirable in a food processing environment.…”

Section: Biosensor Characterizationmentioning

confidence: 99%

Actuation of chitosan-aptamer nanobrush borders for pathogen sensing

Hills

Oliveira

Cavallaro

et al. 2018

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We demonstrate a sensing mechanism for rapid detection of Listeria monocytogenes in food samples using the actuation of chitosan-aptamer nanobrush borders. The bio-inspired soft material and sensing strategy mimic natural symbiotic systems, where low levels of bacteria are selectively captured from complex matrices. To engineer this biomimetic system, we first develop reduced graphene oxide/nanoplatinum (rGO-nPt) electrodes, and characterize the fundamental electrochemical behavior in the presence and absence of chitosan nanobrushes during actuation (pH-stimulated osmotic swelling). We then characterize the electrochemical behavior of the nanobrush when receptors (antibodies or DNA aptamers) are conjugated to the surface. Finally, we test various techniques to determine the most efficient capture strategy based on nanobrush actuation, and then apply the biosensors in a food product. Maximum cell capture occurs when aptamers conjugated to the nanobrush bind cells in the extended conformation (pH < 6), followed by impedance measurement in the collapsed nanobrush conformation (pH > 6). The aptamer-nanobrush hybrid material was more efficient than the antibody-nanobrush material, which was likely due to the relatively high adsorption capacity for aptamers. The biomimetic material was used to develop a rapid test (17 min) for selectively detecting L. monocytogenes at concentrations ranging from 9 to 107 CFU mL-1 with no pre-concentration, and in the presence of other Gram-positive cells (Listeria innocua and Staphylococcus aureus). Use of this bio-inspired material is among the most efficient for L. monocytogenes sensing to date, and does not require sample pretreatment, making nanobrush borders a promising new material for rapid pathogen detection in food.

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Section: Biosensor Characterizationmentioning

confidence: 99%

Actuation of chitosan-aptamer nanobrush borders for pathogen sensing

Hills

Oliveira

Cavallaro

et al. 2018

Analyst

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“…In literature, many rapid analysis methods were developed for L. monocytogenes detection. Alhogail et al designed colorimetric biosensor to detect rapidly the amidolytic activity of Listeria protease [38]. The detection limit was found to be 2.17 × 10 2 cfu/mL in milk and meat samples.…”

Section: Discussionmentioning

confidence: 99%

Immunomagnetic separation and Listeriamonocytogenes detection with surface-enhanced Raman scattering

Akçinar¹,

Aslım²,

Torul³

et al. 2020

Turk J Med Sci

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Background/aim: We aimed to develop a rapid method to enumerate Listeria monocytogenes (L. monocytogenes) utilizing magnetic nanoparticle based preconcentration and surface-enhanced Raman spectroscopy measurements. Materials and methods: Biological activities of magnetic Au-nanoparticles have been observed to have the high biocompatibility, and a sample immunosensor model has been designed to use avidin attached Au-nanoparticles for L. monocytogenes detection. Staphylococcus aureus (S. aureus) and Salmonella typhimurium (S. typhimurium) bacteria cultures were chosen for control studies. Antimicrobial activity studies have been done to identify bio-compatibility and bio-characterization of the Au-nanoparticles in our previous study and capturing efficiencies to bacterial surfaces have been also investigated. Results: We constructed the calibration graphs in various population density of L. monocytogenes as 2.2 × 10 1 to 2.2 × 10 6 cfu/mL and the capture efficiency was found to be 75%. After the optimization procedures, population density of L. monocytogenes and Raman signal intensity showed a good linear correlation (R 2 = 0.991) between 10 2 to 10 6 cfu/mL L. monocytogenes. The presented sandwich assay provides low detection limits and limit of quantification as 12 cfu/mL and 37 cfu/mL, respectively. We also compared the experimental results with reference plate-counting methods and the practical utility of the proposed assay is demonstrated using milk samples. Conclusion: It is focused on the enumeration of L. monocytogenes in milk samples and the comparision of results of milk analysis obtained by the proposed SERS method and by plate counting method stay in food agreement. In the present study, all parameters were optimized to select SERS-based immunoassay method for L. monocytogenes bacteria to ensure LOD, selectivity, precision and repeatablity.

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“…The availability of such methods would enable better control of such bacteria in milk, improving the quality of dairy products and decreasing the economic costs associated with rejection of spoiled milk. Bacteriological methods, immunological methods, and molecular methods, such as multiplex PCR and real-time PCR, have all been applied to the detection of microorganisms in food (Alhogail et al, 2016;Nagaraj et al, 2016;Xu et al, 2017). However, these methods are not always feasible for use on farm or in modest-sized microorganism testing laboratories because they are often expensive and require the use of bulky apparatus.…”

Section: Discussionmentioning

confidence: 99%

Development of a novel loop-mediated isothermal amplification assay for the detection of lipolytic Pseudomonas fluorescens in raw cow milk from north China

Liang

Zhang

et al. 2017

Journal of Dairy Science

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Lipases secreted by psychrotrophic bacteria are known to be heat resistant and can remain active even after the thermal processing of milk products. Such enzymes are able to destabilize the quality of milk products by causing a rancid flavor. Rapid detection of a small amount of heat-resistant lipase-producing psychrotrophic bacteria is crucial for reducing their adverse effects on milk quality. In this study, we established and optimized a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Pseudomonas fluorescens in raw cow milk, as the most frequently reported heat-resistant lipase-producing bacterial species. Pseudomonas fluorescens-specific DNA primers for LAMP were designed based on the lipase gene sequence. Reaction conditions of the LAMP assay were tested and optimized. The detection limit of the optimized LAMP assay was found to be lower than that of a conventional PCR-based method. In pure culture, the detection limit of the LAMP assay was found to be 4.8 × 10 cfu/reaction of the template DNA, whereas the detection limit of the PCR method was 4.8 × 10 cfu/reaction. Evaluation of the performance of the method in P. fluorescens-contaminated pasteurized cow milk revealed a detection limit of 7.4 × 10 cfu/reaction, which was 10 lower than that of the PCR-based method. If further developed, the LAMP assay could offer a favorable on-farm alternative to existing technologies for the detection of psychotrophic bacterial contamination of milk, enabling improved quality control of milk and milk products.

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Rapid colorimetric sensing platform for the detection of Listeria monocytogenes foodborne pathogen

Cited by 117 publications

References 32 publications

Actuation of chitosan-aptamer nanobrush borders for pathogen sensing

Actuation of chitosan-aptamer nanobrush borders for pathogen sensing

Immunomagnetic separation and Listeriamonocytogenes detection with surface-enhanced Raman scattering

Development of a novel loop-mediated isothermal amplification assay for the detection of lipolytic Pseudomonas fluorescens in raw cow milk from north China

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