Rapid communication: dinucleotide repeat polymorphism of rainbow trout, FGT4

Sakamoto, Takashi; Okamoto, Nobuaki; Ikeda, Yoshichika

doi:10.2527/1994.72102767x

Cited by 3 publications

(2 citation statements)

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“…) reflect the way loci were added in the computations, i.e., from most to least informative for maximum fgu estimates and from least to most informative for minimumfgu estimates. For any number ofloci, minimum and maximumfgu values in turbot were higher than minimum and maximumfgu Morris et al (1996), and loci FGT2, FGT3, and FGT4 were c1onedby Sakamoto et al (1994aSakamoto et al ( , 1994bSakamoto et al ( , 1994c.The renaming of loci PuPuPy and Omy77 isjustified by the fact that new primers were designed from tbe published sequences oftbe flanking regions. Brown trout microsatellite locus Str2INRA was c1onedby Estoup et al (1993).T;;l' anneding temperature.…”

Section: Influence Of the Number Of Loci And Of The Mating Scheme Strmentioning

confidence: 99%

Parentage assignment using microsatellites in turbot (Scophthalmus maximus) and rainbow trout (Oncorhynchus mykiss) hatchery populations

Estoup¹,

Gharbi²,

SanCristobal³

et al. 1998

Can. J. Fish. Aquat. Sci.

130

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Eight turbot (Scophthalmus maximus) and eight rainbow trout (Oncorhynchus mykiss) microsatellites were selected for parentage assignment in fish-farmed populations. The number of alleles, gene diversity, polymorphic information content, and the probabilities of exclusion of these loci were 8, 0.76, 0.73, and 0.55 in turbot and 4, 0.65, 0.59 and 0.39 in rainbow trout, respectively. The power of the markers for parentage assignment was assessed by computing the frequency of good and unique decisions (fgu) in a population of genitors defined by its allele frequencies and assuming three different types of mating schemes. The eight turbot microsatellites gave larger maximal mating schemes (the largest mating structure with a fgu >= 0.95) than the eight rainbow trout loci: 1 female (F) mated to 520 males (M) (paternity retrieval scheme), more than 140F x 140M (factorial scheme), and more than 15 000 independent pairs (natural population scheme) for turbot, and 1F x 88M, 34F x 34M, and 7000 independent pairs for rainbow trout. The variation of the fgu values with the number of loci confirmed that the turbot set of microsatellites was more efficient for parentage assignment than the rainbow trout markers.

show abstract

Section: Influence Of the Number Of Loci And Of The Mating Scheme Strmentioning

confidence: 99%

Parentage assignment using microsatellites in turbot (Scophthalmus maximus) and rainbow trout (Oncorhynchus mykiss) hatchery populations

Estoup¹,

Gharbi²,

SanCristobal³

et al. 1998

Can. J. Fish. Aquat. Sci.

130

View full text Add to dashboard Cite

show abstract

“…disease resistance, we are attempting to iso late DNA markers from the rainbow trout genome. [17][18][19][20][21] In fisheries sciences, many species need to be analyzed. If DNA markers isolated from one species can be used for different species, they must be a more profitable tool for ge nome analysis in fisheries sciences.…”

Section: Introductionmentioning

confidence: 99%

Application of PCR Primer Pairs from Rainbow Trout to Detect Polymorphisms of CA Repeat DNA Loci in Five Confamilial Species

1996

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Dinucleotide repeat polymorphisms are widely used as markers for genetic analysis in mammalian genomes because of their abundance and high variability among individuals. We have previously isolat ed informative markers for genetic studies of rainbow trout. In fisheries science, many species need to be analyzed. Therefore, heterologous applications of DNA markers designed in one species can be use ful. The sequences flanking a dinucleotide repeat are often conserved among closely related species. We thus wanted to determine whether DNA markers isolated from the rainbow trout genome could be used to detect dinucleotide repeat polymorphisms in five other species of salmonid. Five sets of primer pairs that flank rainbow trout microsatellites and that produced polymorphic PCR products, were tested with template DNA from five species. Specific PCR products were obtained in all cases. Four out of five DNA markers could detect polymorphism in all species and all five DNA markers could detect polymor phism in at least one species. In many cases, these markers showed large numbers of alleles and high heterozygosity values in other salmonids such as rainbow trout. Our results show that DNA markers iso lated from rainbow trout are also useful in other salmonids for fisheries sciences such as population stu dies and genetic linkage analysis.

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Linkage analysis of quantitative trait loci associated with spawning time in rainbow trout (Oncorhynchus mykiss)

et al. 1999

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Rapid communication: dinucleotide repeat polymorphism of rainbow trout, FGT4

Cited by 3 publications

References 0 publications

Parentage assignment using microsatellites in turbot (Scophthalmus maximus) and rainbow trout (Oncorhynchus mykiss) hatchery populations

Parentage assignment using microsatellites in turbot (Scophthalmus maximus) and rainbow trout (Oncorhynchus mykiss) hatchery populations

Application of PCR Primer Pairs from Rainbow Trout to Detect Polymorphisms of CA Repeat DNA Loci in Five Confamilial Species

Linkage analysis of quantitative trait loci associated with spawning time in rainbow trout (Oncorhynchus mykiss)

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