Rapid comparison of metabolic equivalence of standard medicinal parts from medicinal plants and their in vitro-generated adventitious roots using FT-IR spectroscopy

Abstract

To determine whether metabolite fingerprinting for whole cell extracts based on Fourier transform infrared (FT-IR) spectroscopy can be used to discriminate and compare metabolic equivalence, standard medicinal parts from four medicinal plants (Cynanchum wilfordii Hemsley, Atractylodes japonica Koidz, Polygonum multiflorum Thunberg and Astragalus membranaceus Bunge) and their in vitro-produced adventitious roots were analyzed by FT-IR spectroscopy. The principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) from the FT-IR spectral data showed that the whole metabolic pattern from Cynanchum wilfordii was highly similar to Astragalus membranaceus. However, Atractylodes japonica and Polygonum multiflorum showed significantly different metabolic patterns. Furthermore, adventitious roots from Cynanchum wilfordii and Astragalus membranaceus also showed similar metabolic patterns compared to their standard medicinal parts. These results clearly show that mass proliferation of adventitious roots may be applied to aquire novel supply of standard medicinal parts from medicinal plants. However, the whole metabolic pattern from adventitious roots of Atractylodes japonica and Polygonum multiflorum were not similar to their standard medicinal parts. Furthermore, FT-IR spectroscopy combined with multivariate analyses established in this study may be applied as an alternative tool to discriminate the whole metabolic equivalence from several standard medicinal parts. Thus, we suggest that these metabolic discrimination systems may be applied for metabolic standardization of herbal medicinal resources.

Metabolic Discrimination between Adventitious Roots and Standard Medicinal Part of Atractylodes macrocephala Koidz. Using FT-IR Spectroscopy

“…FT-IR spectral analysis is widely used to identify closely related microbial species [15,16], for the classification of plant species [17], and for cultivar identification [18,19]. Moreover, FT-IR spectral analysis can be applied in the comparison of the metabolic similarity between standard medicinal parts and their in vitro proliferated adventitious roots [20,21].…”

Rapid metabolic discrimination betweenZoysia japonicaandZoysia sinicabased on multivariate analysis of FT-IR spectroscopy

“…최근 FT-IR (Fourier transform infrared spectroscopy)의 대사 체 분석기술을 이용하여 대사체 수준에서 생물 종 식별이 보고되었다 (Krishnan et al 2005). 적외선분광분석(FT-IR)은 대사체 수준에서 시료간의 양적 및 질적 패턴 차이를 조사 하는 연구 분야로, 시료의 전세포추출물에 대한 적외선분 광 스펙트럼데이터를 다변량 통계분석기법을 통해 생물종 간의 유연관계를 식별할 수 있다 (Ahn et al 2015;Kim et al 2004;Kim et al 2007;Kim et al 2009;Kwon et al 2011;Kwon et al 2014 (Fig. 2B).…”