Rapid, cost-effective and organic solvent-free production of biologically active essential oil from Mediterranean wild Origanum syriacum

Shehadeh, Mayadah B.; Jaradat, Nidal; Al-Masri, Motasem; Zaid, Abdel Naser; Hussein, Fatima; Khasati, Ahmad; Suaifan, Ghadeer A. R. Y.; Darwish, Rula M.

doi:10.1016/j.jsps.2019.03.001

Cited by 22 publications

(31 citation statements)

References 31 publications

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“…They have specific aromatic odors and evaporate at room temperatures. VOs are frequently used for therapeutic purposes due to their antibacterial, antifungal, anthelmintic, analgesic, antipruritic and anticancer effects [27]. These natural compounds are isolated from various plant parts and tissues and they belong to several groups of phytochemical constituents such as aldehydes, esters, ethers, hydrocarbons, phenols, ketones, and oxides [28].…”

Section: Discussionmentioning

confidence: 99%

Composition, Anticholinesterase and Antipedicular Activities of Satureja capitata L. Volatile Oil

et al. 2020

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AbstractThe emergence of resistance for antipedicular agents and the need of potent acetylcholinesterase (AChE) therapeutics for the treatment of a neurodegenerative disorder such as Alzheimer disease has led researchers to the exploration of new therapeutic alternatives such as natural volatile oils. Therefore, the current investigation aimed to identify the components of Satureja capitata L. volatile oil (VO), and examine the VO anticholinesterase, and antipedicular activities. The plant phytoconstituents were identified using Gas chromatography mass spectrometry (GC-MS) method, while the anticholinesterase activity was determined against butyryl- and acetyl-cholinesterase using Ellman’s method. In addition, antipedicular activity was established using the diffusion method. The obtained GC-MS results identified 16 compounds in S. capitata VO with the major constituents being carvacrol, γ-terpinene, and p-cymene. Anticholinesterase analysis showed a marked inhibition potential against acetyl- and butyryl-cholinesterase enzymes with half maximal inhibitory concentration (IC50) values of 28.24±0.97 μg/ml and 92.31±1.22 μg/ml, respectively in comparison with the reference compound galantamine, which has IC50 values against the same enzymes of 5.21±0.07 μg/ml and 10.33±0.37 μg/ml, respectively. In addition, the VO, at a concentration of 20%, was effective against head lice, similar to benzyl benzoate, which resulted in 100% mortality. In addition, the VO completely inhibited the emergence of lice nits after 6 and 14 days. On the basis of the obtained results, S. capitata VO is a promising natural alternative to synthetic antipedicular and anticholinesterase drugs, which can be employed in drug development, and may lead to new candidates against head lice and neurodegenerative diseases.

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Section: Discussionmentioning

confidence: 99%

Composition, Anticholinesterase and Antipedicular Activities of Satureja capitata L. Volatile Oil

et al. 2020

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“…That means the go-back to a preantibiotic epoch in which infectious diseases caused by multiple-resistant microbial pathogens are uncontrollable [ 29 ]. It is well documented that EOs displayed a comparable antimicrobial effect against the tested antibiotic-resistant and antibiotic-susceptible strains; therefore, it could be presumed that the EOs mechanism of action is unlike that from the used antibacterial and antifungal drugs [ 11 ]. The antimicrobial activity of the EO extracted from G. curviflora leaves and flowers was assessed using broth microdilution assay.…”

Section: Discussionmentioning

confidence: 99%

“…After washing with distilled water, the leaves and flowers were left to dry in the shadow at a 25 ± 2°C temperature and 55 ± 5 RH humidity for two weeks then powdered using a mechanical grinder and stored in paper bags [ 10 ]. The G. curviflora EO was extracted utilizing the Ultrasonic-Microwave apparatus according to the literature procedure [ 11 ]. A 1 L round-bottom flask containing 50 g of the dried plant powder and 0.5 L of distilled water was positioned in the apparatus and connected to the Clevenger apparatus.…”

Section: Methodsmentioning

confidence: 99%

Glechoma curviflora Volatile Oil from Palestine: Chemical Composition and Neuroprotective, Antimicrobial, and Cyclooxygenase Inhibitory Activities

Al‐Maharik

Jaradat

Qneibi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The rise of the emergence of microbial resistance of antibiotics, the dangerous side effects of nonsteroidal anti-inflammatory drugs, and noncompetent medications of Alzheimer’s, Parkinson’s, and other neurodegenerative diseases prompt scientists to search for phytochemicals that could be utilized in the remedy of lethal diseases. Glechoma curviflora (Boiss.) Kuntze (Nepeta curviflora) is a medicinal herb growing in the eastern parts of the Mediterranean Sea Basin and is widely consumed as a tea. The leaves of this plant have been traditionally used for the treatment of various infectious diseases. The current research was designed to identify the chemical composition of Glechoma curviflora (Boiss.) essential oil (EO) and to assess its antibacterial, antifungal, and cyclooxygenase inhibitory activities and the biophysical gating effect on AMPA receptors. Twenty phytochemicals were identified from G. curviflora leaves and flowers EO amounting to almost 100% of the total constituents using GC-MS technique, of which 1,6-dimethylspiro[4.5]decane (27.51%) 1, caryophyllene oxide (20.08%) 2, and β-caryophyllene (18.28%) 3 were the main constituents. The biophysical properties’ effect from the plant extract on various AMPA-type receptors expressed in Human Embryonic Kidney (HEK293) cells was assessed by exploiting the whole-cell patch-clamp technique. Microdilution assay was adopted for assessing the antimicrobial property against eight virulent microbial strains whilst the cyclooxygenase inhibition effect was accomplished utilizing COX inhibitory screening colorimetric assay G. curviflora EO displayed potent activity against P. aeruginosa (MIC = 1.25 μg/mL), S. sonnei (MIC = 3.12 μg/mL), and E. coli (MIC = 1.25 μg/mL), compared with ciprofloxacin (positive control) and potent antibacterial activity against S. aureus, MRSA, S. sonnei, E. coli, and P. aeruginosa compared to Ampicillin (2nd positive control). It also showed anti-Candida (MIC = 6.25 μg/mL) and antimold (MIC = 3.125 μg/mL) activities compared with fluconazole (antifungal positive control). Likewise, our results showed an inhibition and biophysical impact of G. curviflora on all AMPARs subunits.

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“…Later on, S. viticina leaves were cleaned, rinsed at least three times with distilled water and then dried in the shade at 25 ± 2 °C and 55 ± 5 RH of humidity for three weeks. The dried leaves were cut into small pieces which were kept in special paper bags for farther experimental work [27].…”

Section: Methodsmentioning

confidence: 99%

Fingerprinting, Antimicrobial, Antioxidant, Anticancer, Cyclooxygenase and Metabolic Enzymes Inhibitory Characteristic Evaluations of Stachys viticina Boiss. Essential Oil

Jaradat

Al‐Maharik

2019

Molecules

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The present study aimed to identify the chemical constituents and to assess the in-vitro, antimicrobial, anticancer, antioxidant, metabolic enzymes and cyclooxygenase (COX) inhibitory properties of essential oil (EO) of Stachys viticina Boiss. leaves. The S. viticina EO was isolated and identified using microwave-ultrasonic and GC-MS techniques, respectively. Fifty-two compounds were identified, of which endo-borneol was the major component, followed by eucalyptol and epizonarene. The EO was evaluated against a panel of in-vitro bioassays. The EO displayed antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Epidermophyton floccosum, with MIC values of 0.039, 0.078 and 0.78 mg/mL, respectively. The EO exhibited cytotoxicity against HeLa (cervical adenocarcinoma) and Colo-205 (colon) cancer cell lines with percentages of inhibition of 95% and 90%, for EO concentrations of 1.25 and 0.5 mg/mL, respectively. Furthermore, it showed metabolic enzyme (α-amylase, α-glucosidase, and lipase) inhibitory (IC50 = 45.22 ± 1.1, 63.09 ± 0.26, 501.18 ± 0.38 µg/mL, respectively) and antioxidant activity, with an IC50 value of 19.95 ± 2.08 µg/mL. Moreover, the S. viticina EO showed high cyclooxygenase inhibitory activity against COX-1 and COX-2 with IC50 values of 0.25 and 0.5 µg/mL, respectively, similar to those of the positive control (the NSAID etodolac). Outcomes amassed from this investigation illustrate that S. viticina EO represents a rich source of pharmacologically active molecules which can be further validated and explored clinically for its therapeutic potential and for the development and design of new natural therapeutic preparations.

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Rapid, cost-effective and organic solvent-free production of biologically active essential oil from Mediterranean wild Origanum syriacum

Cited by 22 publications

References 31 publications

Composition, Anticholinesterase and Antipedicular Activities of Satureja capitata L. Volatile Oil

Composition, Anticholinesterase and Antipedicular Activities of Satureja capitata L. Volatile Oil

Glechoma curviflora Volatile Oil from Palestine: Chemical Composition and Neuroprotective, Antimicrobial, and Cyclooxygenase Inhibitory Activities

Fingerprinting, Antimicrobial, Antioxidant, Anticancer, Cyclooxygenase and Metabolic Enzymes Inhibitory Characteristic Evaluations of Stachys viticina Boiss. Essential Oil

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