Rapid degradation of bacteriophage λ O protein by ClpP/ClpX protease influences the lysis-versus-lysogenization decision of the phage under certain growth conditions of the host cells

Czyż, Agata; Zielke, Ryszard A.; Węgrzyn, Grzegorz

doi:10.1007/s007050170073

Cited by 9 publications

(9 citation statements)

References 14 publications

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“…As reported previously for wild-type plasmids replicating in wild-type E. coli (Barajska et al 2002), in all hosts employed in this experiment, intermediates characteristic for both bidirectional and unidirectional replication modes could be observed with roughly equal frequency. Therefore, we conclude that dysfunction of ClpP/ClpX protease (and resultant stabilization of the O protein, as demonstrated previously by Gottesman et al 1993;Wdgrzyn et al 2000;Czyr et al 2001) has no signiWcant eVect on directionality of DNA replication.…”

Section: Resultssupporting

confidence: 60%

Switch from θ to σ replication of bacteriophage λ DNA: factors involved in the process and a model for its regulation

et al. 2007

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Bacteriophage lambda genome is one of the classical model replicons in studies on the regulation of DNA replication. Moreover, since genes coding for Shiga toxins are located in genomes of lambdoid phages, understanding of mechanisms controlling lambda DNA replication may be of bio-medical importance. During lytic development of bacteriophage lambda, its genome is replicated according to the theta (circle-to-circle) mode early after infection, and then it is switched to the sigma (rolling circle) mode. Two mechanisms of regulation of this switch were proposed recently and both suggested a crucial role for directionality of lambda DNA replication. Whereas one hypothesis assumed transient impairment of ClpP/ClpX-mediated proteolysis of the lambdaO initiator protein, another suggested a crucial role for transcriptional activation of the orilambda region and factors involved in the control of the p (R) promoter activity. Here we demonstrate that mutations in clpP and clpX genes had little influence on both directionality of lambda DNA replication and appearance of sigma replication intermediates. On the other hand, regulators affecting activity of the p (R) promoter (responsible for initiation of transcription, which activates orilambda) directly or indirectly influenced directionality of lambda DNA replication to various extents. Therefore, we conclude that regulation of the efficiency of transcriptional activation of orilambda, rather than transient impairment of the lambdaO proteolysis, is responsible for the control of the switch from theta to sigma replication, and propose a model for this control.

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Section: Resultssupporting

confidence: 60%

Switch from θ to σ replication of bacteriophage λ DNA: factors involved in the process and a model for its regulation

et al. 2007

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“…Stability of the cII gene product, expressed from plasmid pMO23, in E. coli cells was investigated in pulse–chase experiments followed by cell lysis, SDS‐PAGE, autoradiography and densitometry of appropriate bands on autoradiograms, according to a previously described method (Czyż et al ., 2001).…”

Section: Methodsmentioning

confidence: 99%

“…The 'lysis-versus-lysogenization' decision is a crucial step in the phage life cycle, and there are many conditions that influence the choice of one of the two alternative developmental modes of the infecting l phage. Low multiplicity of infection (MOI) favours the lytic cycle, in contrast to high MOI, which stimulates lysogenization (for reviews, see Taylor and Wegrzyn, 1998;W grzyn et al ., 2001). During infection at low temperature, the lytic cycle is blocked, whereas lysogenization proceeds efficiently (Obuchowski et al ., 1997a;Gabig et al ., 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Growth rate of the host cell is another factor influencing the lysis-versus-ę n ¢ lysogenization decision. Higher efficiency of lysogenization is observed when host cells are cultured in poor growth conditions than during bacterial growth in rich media (Taylor and Wegrzyn, 1998;W grzyn et al ., 2001). The influence of nutritional growth conditions on this decision is based on the actions of specific nucleotide alarmones, cAMP and ppGpp.…”

Section: Introductionmentioning

confidence: 99%

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SeqA‐mediated stimulation of a promoter activity by facilitating functions of a transcription activator

Słomińska

Konopa

Ostrowska

et al. 2003

Molecular Microbiology

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SummaryIt was demonstrated recently that the SeqA protein, a main negative regulator of Escherichia coli chromosome replication initiation, is also a specific transcription factor. SeqA specifically activates the bacteriophage l p R promoter while revealing no significant effect on the activity of another l promoter, p L . Here, we demonstrate that lysogenization by bacteriophage l is impaired in E. coli seqA mutants. Genetic analysis demonstrated that CII-mediated activation of the phage p I and p aQ promoters, which are required for efficient lysogenization, is less efficient in the absence of seqA function. This was confirmed in in vitro transcription assays. Interestingly, SeqA stimulated CII-dependent transcription from p I and p aQ when it was added to the reaction mixture before CII, although having little effect if added after a preincubation of CII with the DNA template. This SeqA-mediated stimulation was absolutely dependent on DNA methylation, as no effects of this protein were observed when using unmethylated DNA templates. Also, no effects of SeqA on transcription from p I and p aQ were observed in the absence of CII. Binding of SeqA to templates containing the tested promoters occurs at GATC sequences located downstream of promoters, as revealed by electron microscopic studies. In contrast to p I and p aQ , the activity of the third CII-dependent promoter, p E , devoid of neighbouring downstream GATC sequences, was not affected by SeqA both in vivo and in vitro . We conclude that SeqAstimulates transcription from p I and p aQ promoters in co-operation with CII by facilitating functions of this transcription activator, most probably by allowing more efficient binding of CII to the promoter region.

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“…Under standard laboratory conditions, inactivation of ClpXP protease has no significant effect on k growth. However, degradation of O protein may play a role in the lysis-lysogeny decision in slow growing bacterial cells (Czyz et al, 2001). N-terminal sequences play the most critical role in engaging proteolysis by ClpP/ClpX (Gonciarz-Swiatek et al, 1999).…”

Section: The Participation Of Proteases In Phage Dna Replicationmentioning

confidence: 99%

Recruitment of host ATP-dependent proteases by bacteriophage λ

Kobiler

Oppenheim

Herman

2004

Journal of Structural Biology

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Rapid degradation of bacteriophage λ O protein by ClpP/ClpX protease influences the lysis-versus-lysogenization decision of the phage under certain growth conditions of the host cells

Cited by 9 publications

References 14 publications

Switch from θ to σ replication of bacteriophage λ DNA: factors involved in the process and a model for its regulation

Switch from θ to σ replication of bacteriophage λ DNA: factors involved in the process and a model for its regulation

SeqA‐mediated stimulation of a promoter activity by facilitating functions of a transcription activator

Recruitment of host ATP-dependent proteases by bacteriophage λ

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