Rapid densitometric determination of cell migration and cell adhesion in a microchemotaxis chamber

Abstract

A new rapid staining and measuring method has been developed for the quantification of migrated cells in a microchemotaxis chamber. The migrated cells were, after staining, evaluated by a transmission densitometer. The method introduced here is more accurate and faster than those described previously. In addition the technique can be used to determine the adherent capacity of cells.

Evaluation of Neutrophil Motility by Image Analysis

“…So some modern approaches capable of acquiring and analyzing more data have been proposed, including computer‐assisted systems. In some cases they were applied to den‐sitometry 21,22 ; in some others, to image analysis devices. Most of image analysis devices 23,24 have been applied in agarose assays 25 or were used to detect the orientation of a single cell or a small number of cells in experimental models 26 .…”