Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS

Hoyos‐Mallecot, Yannick; Riazzo, Cristina; Miranda-Casas, Consuelo; Rojo-Martín, María Dolores; Gutiérrez‐Fernández, José; Navarro-Marí, José María

doi:10.1016/j.mimet.2014.07.016

Cited by 43 publications

(27 citation statements)

References 17 publications

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“…) described a sensitivity of 98·9% and a specificity of 97·8% compared with PCR in a MALDI‐TOF MS to OXA‐48 producing Enterobacteriaceae, where an imipenem substrate was used after 1 h incubation with bacterial extracts and a turnaround time of 90 min (Hoyos‐Mallecot et al . ; Sauget et al . ).…”

Section: Phenotypic Detection Methodsmentioning

confidence: 98%

“…Hoyos‐Mallecot et al . () used blood cultures to determine the presence of carbapenemases with a MALDI‐TOF MS, and obtained a sensitivity and specificity of 100 and 90% respectively within 4·5 h. Sauget and colleagues (Sauget et al . ) described a sensitivity of 98·9% and a specificity of 97·8% compared with PCR in a MALDI‐TOF MS to OXA‐48 producing Enterobacteriaceae, where an imipenem substrate was used after 1 h incubation with bacterial extracts and a turnaround time of 90 min (Hoyos‐Mallecot et al .…”

Section: Phenotypic Detection Methodsmentioning

confidence: 99%

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Review of established and innovative detection methods for carbapenemase-producing Gram-negative bacteria

2015

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SummaryThe minimal antibiotic options for carbapenemase-producing Gram-negative bacteria necessitate their rapid detection. A literature review of a variety of phenotypic and genotypic methods is presented. Advances in culture methods and screening media are still subject to long incubation hours. Biochemical methods have shorter turnaround times and higher sensitivities and specificities, but cannot differentiate between various types and variants. Spectrophotometric methods are cheap and efficient, but are uncommon in many clinical settings, while the MALDI-TOF MS is promising for species identification, typing and resistance gene determination. Although next generation sequencing (NGS) technologies provide a better platform to detect, type and characterize carbapenem-resistant bacteria, the different NGS platforms, the large computer memories and space needed to process and store genomic data and the nonuniformity in data analysis platforms are still a challenge. The sensitivities, specificities and turnaround times recorded in the various studies reviewed favours the use of the biochemical tests (Carba NP or Rapid Carb screen tests) for the detection of putative carbapenemaseproducing isolates. MALDI-TOF MS and/or molecular methods like microarray, loop-mediated isothermal amplification and real-time multiplex PCR assays could be used for further characterization in a reference laboratory. NGS may be used for advanced epidemiological and molecular studies.

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Section: Phenotypic Detection Methodsmentioning

confidence: 98%

Section: Phenotypic Detection Methodsmentioning

confidence: 99%

Review of established and innovative detection methods for carbapenemase-producing Gram-negative bacteria

2015

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“…Mass spectrometers manufacturers, such as Bruker Daltonics © (https://www.bruker.com/) propose software platforms (e.g., FlexControl™, FlexAnalysis™, Maldi Biotyper Compass Explorer™ and Clinpro Tools™) allowing the acquisition, processing of spectra and the creation of customized databases, and together with other bioinformatics pipelines provide new performant tools to the MALDI-TOF MS community [70,71]. Applied Maths NV © (http://www.applied-maths.com/bionumerics), notably, proposes BioNumerics™, a pipeline platform for advanced analysis of spectra.…”

Section: Bioinformatics: a Powerful Tool To Reinforce Diagnosticsmentioning

confidence: 99%

MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens

2019

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Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is today the reference method for direct identification of microorganisms in diagnostic laboratories, as it is notably time-and cost-efficient. In the context of increasing cases of enteric diseases with emerging multi-drug resistance patterns, there is an urgent need to adopt an efficient workflow to characterize antimicrobial resistance (AMR). Current approaches, such as antibiograms, are time-consuming and directly impact the "patient-physician" workflow. Through this mini-review, we summarize how the detection of specific patterns by MALDI-TOF MS, as well as bioinformatics, become more and more essential in research, and how these approaches will help diagnostics in the future. Along the same lines, the idea to export more precise biomarker identification steps by MALDI-TOF(/TOF) MS data towards AMR identification pipelines is discussed. The study also critically points out that there is currently still a lack of research data and knowledge on different foodborne pathogens as well as several antibiotics families such as macrolides and quinolones, and many questions are still remaining. Finally, the innovative combination of whole-genome sequencing and MALDI-TOF MS could be soon the future for diagnosis of antimicrobial resistance in foodborne pathogens.

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“…Matrix-assisted laser desorption ionization-time of flight mass spectrometry has been shown to rapidly detect extended beta-lactamases producing Enterobacteriaceae from positive blood cultures (13/141) with a sensitivity of 99% after 90-150 min [19]. The same technique was also effective to detect carbapenemase producing Enterobacteriaceae and Pseudomonas aeruginosa in blood cultures within 4.5 h with high sensitivity and specificity [20]. Other molecular diagnostics assays that identify pathogen DNA and resistance markers within hours are also available (fluorescence in situ hybridization and real-time PCR) [21].…”

Section: Combination Therapy May Be Indicated For Lifethreatening Infmentioning

confidence: 99%

Individualized antibiotic strategies

Taccone

Bond²,

Cavicchi³

et al. 2016

Current Opinion in Anaesthesiology

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Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS

Cited by 43 publications

References 17 publications

Review of established and innovative detection methods for carbapenemase-producing Gram-negative bacteria

Review of established and innovative detection methods for carbapenemase-producing Gram-negative bacteria

MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens

Individualized antibiotic strategies

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