Rapid detection and quantification of aminoglycoside phosphorylation products using direct‐infusion high‐resolution and ultra‐high‐performance liquid chromatography/mass spectrometry

Perez, Johnny J.; Chen, Chin-Yi

doi:10.1002/rcm.8241

Cited by 6 publications

(2 citation statements)

References 26 publications

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“…Aminoglycosides and fluorinated surfactants are examples of these compounds. To overcome this obstacle, ion pairing agents have been employed to facilitate separation of aminoglycosides. , Ion pairing reagents are generally avoided, as these reagents require high concentrations and are almost impossible to remove once introduced to the analytical column. In addition, ion pairing reagents change selectivity when attempting to employ that column for non-ion-pair applications.…”

Section: Introductionmentioning

confidence: 99%

Rapid Quantification of Ammonium Nitrate and Urea Nitrate Using Liquid Chromatography–High-Resolution Orbitrap Mass Spectrometry

Perez,

Brady,

Broderick

et al. 2024

Anal. Chem.

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Resolution and sensitivity improvements in mass spectrometry technology have enabled renewed attempts at solving challenging analytical issues. One such issue involves the analysis of energetic ionic species. Energetic ionic species make up an important class of chemical materials, and a more robust and versatile analytical platform would provide tremendous value to the analytical community. Initial attempts at quantification of energetic ionic species employed high-resolution time-of-flight measurements with crown ether (CE) complexation and flow injection analysis (FIA). In this investigation, ammonium nitrate (AN) and urea nitrate (UN) in the presence of a crown ether complexation agent were explored by using high-resolution orbitrap mass spectrometry. Product ion scans of these signature complexes reveal positive identification of these energetic ionic species. Finally, quantification was demonstrated for both flow injection and liquid chromatography−mass spectrometry (LC-MS) analysis, suggesting the capability for routine and rapid analysis of these energetic ionic materials.

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Section: Introductionmentioning

confidence: 99%

Rapid Quantification of Ammonium Nitrate and Urea Nitrate Using Liquid Chromatography–High-Resolution Orbitrap Mass Spectrometry

Perez,

Brady,

Broderick

et al. 2024

Anal. Chem.

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“…The European Union (EU) stipulated 150 μg/kg as the maximum residue limit (MRL) for KAN in milk (European Commission, 2010). Thus far, numerous analytical methods have been reported for the detection of KAN residues in milk, including high‐performance liquid chromatography (HPLC) (Oertel et al ., 2004; Perez & Chen, 2018; Zhang et al ., 2019a, 2019b), LC (Acaroz et al ., 2020; Tasci et al ., 2021), immunoassay (Jiang et al ., 2018) and aptasensors (Sun et al ., 2014; Zou et al ., 2021). The limitations of these instrument methods include time‐consuming processes, tedious sample pretreatments and expensive instrument requirements, which may hinder their applications in the rapid, sensitive and high‐throughput screenings of contaminants in food.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle‐carbon nanotube nanohybrids with peroxidase‐like activity for the highly‐sensitive immunoassay of kanamycin in milk

Zeng

Chen

et al. 2022

Int J of Food Sci Tech

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Summary Owing to the increasing incidences of antibiotic residues being found in foods of animal‐origin, rapid and reliable screening methods for detecting these residues are urgently required. This study aimed to develop a robust and ultrasensitive colorimetric immunoassay based on nanohybrids for the rapid screening of kanamycin (KAN) residues in milk. The HAuCl4 solution was directly reduced on the multiwalled carbon nanotubes (MWCNTs) using a one‐step synthesis method to obtain the Au/MWCNTs nanohybrids. The Au/MWCNTs exhibited mimetic peroxidase activity and higher affinities for the TMB and H2O2 compared with horseradish peroxidase and other nanohybrids. A direct competitive enzyme‐linked immunosorbent assay (dcELISA) based on Au/MWCNTs was developed and employed for KAN detection in milk samples under optimised conditions. The dcELISA showed high sensitivity toward KAN residues, with a detection limit of 11.2 pg/mL. Moreover, this novel immunoassay displayed high precision and accuracy in milk samples, with a recovery rate ranging from 94.3 to 124.5% and a coefficient variation from 7.8 to 11.3%. Eight real milk samples were found to contain a KAN concentration in the range of ND–0.26 ng/mL. Therefore, this dcELISA based on the Au/MWCNTs nanohybrid has promising potential for applications in food monitoring and quality control.

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Implementation of normalized retention time (iRT) for bottom-up proteomic analysis of the aminoglycoside phosphotransferase enzyme facilitating method distribution

Perez

Chen

2018

Anal Bioanal Chem

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Rapid detection and quantification of aminoglycoside phosphorylation products using direct‐infusion high‐resolution and ultra‐high‐performance liquid chromatography/mass spectrometry

Cited by 6 publications

References 26 publications

Rapid Quantification of Ammonium Nitrate and Urea Nitrate Using Liquid Chromatography–High-Resolution Orbitrap Mass Spectrometry

Rapid Quantification of Ammonium Nitrate and Urea Nitrate Using Liquid Chromatography–High-Resolution Orbitrap Mass Spectrometry

Gold nanoparticle‐carbon nanotube nanohybrids with peroxidase‐like activity for the highly‐sensitive immunoassay of kanamycin in milk

Implementation of normalized retention time (iRT) for bottom-up proteomic analysis of the aminoglycoside phosphotransferase enzyme facilitating method distribution

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