Rapid Detection and Species Identification of Mycobacteria in Paraffin-Embedded Tissues by Polymerase Chain Reaction

Ghossein, Ronald; Ross, Donald; Salomon, Robert N.; Rabson, Arthur R.

doi:10.1097/00019606-199209000-00005

Cited by 19 publications

(24 citation statements)

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“…Additionally, this technique has been advantageous when the mycobacteria fail to grow in culture because of small numbers or the fastidious nature of the organism, as well as in cases wherein mycobacterial disease was not initially considered. Moreover, an amplification assay can potentially give a result much faster (within 2 to 3 days) than can culture (up to 6 weeks) (60,126).…”

Section: Molecular Identificationmentioning

confidence: 99%

“…Earlier reports by Ghossein et al (60), described a method in which amplified fragments from paraffin-embedded tissue, as well as cultures of M. tuberculosis, M. avium complex, and "saprophytic mycobacteria," were identified by PCR of a 383-bp segment of the gene encoding the 65-kDa mycobacterial surface antigen and subsequent digestion with NarI. Unfortunately, the "saprophytic mycobacteria" were not further identified by the authors (60).…”

Section: Molecular Identificationmentioning

confidence: 99%

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Clinical and Taxonomic Status of Pathogenic Nonpigmented or Late-Pigmenting Rapidly Growing Mycobacteria

2002

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The history, taxonomy, geographic distribution, clinical disease, and therapy of the pathogenic nonpigmented or late-pigmenting rapidly growing mycobacteria (RGM) are reviewed. Community-acquired disease and health care-associated disease are highlighted for each species. The latter grouping includes health care-associated outbreaks and pseudo-outbreaks as well as sporadic disease cases. Treatment recommendations for each species and type of disease are also described. Special emphasis is on the Mycobacterium fortuitum group, including M. fortuitum, M. peregrinum, and the unnamed third biovariant complex with its recent taxonomic changes and newly recognized species (including M. septicum, M. mageritense, and proposed species M. houstonense and M. bonickei). The clinical and taxonomic status of M. chelonae, M. abscessus, and M. mucogenicum is also detailed, along with that of the closely related new species, M. immunogenum. Additionally, newly recognized species, M. wolinskyi and M. goodii, as well as M. smegmatis sensu stricto, are included in a discussion of the M. smegmatis group. Laboratory diagnosis of RGM using phenotypic methods such as biochemical testing and high-performance liquid chromatography and molecular methods of diagnosis are also discussed. The latter includes PCR-restriction fragment length polymorphism analysis, hybridization, ribotyping, and sequence analysis. Susceptibility testing and antibiotic susceptibility patterns of the RGM are also annotated, along with the current recommendations from the National Committee for Clinical Laboratory Standards (NCCLS) for mycobacterial susceptibility testing

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Section: Molecular Identificationmentioning

confidence: 99%

Section: Molecular Identificationmentioning

confidence: 99%

Clinical and Taxonomic Status of Pathogenic Nonpigmented or Late-Pigmenting Rapidly Growing Mycobacteria

2002

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“…9 " 15 Recently, several investigators have used the same methods to identify M tuberculosis from archival formalinfixed paraffin-embedded (FFPE) tissue. 16 " 23 Interestingly, none of the studies that identified mycobacteria from FFPE tissue provided follow-up information for those patients that were positive by PCR but negative by acid-fast stain and culture (AFSC) to determine if any of those patients eventually had positive cultures.…”

mentioning

confidence: 99%

Rapid Detection of Mycobacteria in Inflammatory Necrotizing Granulomas From Formalin-Fixed, Paraffin-Embedded Tissue by PCR in Clinically High-Risk Patients With Acid-Fast Stain and Culture-Negative Tissue Biopsies

et al. 1996

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A collection of inflammatory necrotizing granulomas (INGs) negative by acid-fast stain and culture (AFSC) were analyzed by polymerase chain reaction (PCR) for the presence of mycobacteria. Forty-two paraffin-embedded specimens with INGs were collected from patients at high risk for contracting tuberculosis. Twenty biopsies were positive and 22 were negative for mycobacteria by AFSC. Two universal primers specific for all mycobacteria were used to detected a 414 base pair (bp) fragment of 16S rRNA gene. Twenty of 20 biopsies were positive for mycobacteria by both AFSC and PCR (100%), whereas 19 of 22 biop-A resurgence in tuberculosis has become a very serious public health concern. 1 " 8 In 1992, there were 26,532 new cases in the United States. Georgia ranked 5th among the states with 950 new cases. Grady Memorial Hospital (GMH), a 1,200 bed inner-city hospital in Atlanta, Georgia, where this study took place, had 257 new cases of tuberculosis in 1992, a total higher than that for all but 25 states in the United States. These developments in tuberculosis infection have generated a sense of urgency to develop more efficient methods for detecting mycobacteria.

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“…Such patients usually show evidence of miliary pulmonary TB as well. 3 Isolated liver TB is the rarest form of local hepatic TB, 4 which is reported to have an overall incidence of 0.3%. 5 The local type occurs through the spread of the Mycobacterium by the portal vein from the intestine.…”

Section: Discussionmentioning

confidence: 99%

Portal Hypertension Secondary to Isolated Liver Tuberculosis

Otoukesh¹,

Shahsafi²,

Tahbaz³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. In this report, we present a case of isolated liver tuberculosis (TB) as a cause of non-cirrhotic portal hypertension leading to bleeding esophageal varices. Although TB has been known to cause portal hypertension in a variety of ways, this case was notable for the presence of periportal inflammation and granulomas, also seen in hepatic schistosomiasis. Herein, we discuss isolated liver TB and the differential diagnosis of non-cirrhotic portal hypertension. In endemic areas, TB should be considered in the differential diagnosis of non-cirrhotic portal hypertension.

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Rapid Detection and Species Identification of Mycobacteria in Paraffin-Embedded Tissues by Polymerase Chain Reaction

Cited by 19 publications

References 0 publications

Clinical and Taxonomic Status of Pathogenic Nonpigmented or Late-Pigmenting Rapidly Growing Mycobacteria

Clinical and Taxonomic Status of Pathogenic Nonpigmented or Late-Pigmenting Rapidly Growing Mycobacteria

Rapid Detection of Mycobacteria in Inflammatory Necrotizing Granulomas From Formalin-Fixed, Paraffin-Embedded Tissue by PCR in Clinically High-Risk Patients With Acid-Fast Stain and Culture-Negative Tissue Biopsies

Portal Hypertension Secondary to Isolated Liver Tuberculosis

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