Rapid detection methodology for inorganic mercury (Hg2+) in seafood samples using conjugated polymer (1,4-bis-(8-(4-phenylthiazole-2-thiol)-octyloxy)-benzene) (PPT) by colorimetric and fluorescence spectroscopy

Pavase, Tushar Ramesh; Lin, Hong; Shaikh, Qurat-ul-ain; Liu, Zhenxing

doi:10.1016/j.snb.2015.05.046

Cited by 16 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 10 ] Although these detection methods can accurately and specifically detect Hg 2+ , usually the equipment required is very expensive and needs skilled personnel to operate the procedure. To solve this problem, many Hg 2+ sensors have been designed using small chemical compounds, [ 11–14 ] gold nanoparticles, [ 15 ] DNAzymes, [ 16,17 ] oligonucleotide DNA, and proteins. [ 18 ] Among them, the oligonucleotide DNA biosensor has the advantages of low cost, high stability, and convenience to modify.…”

Section: Introductionmentioning

confidence: 99%

A label‐free ratiometric method to detect Hg²⁺ based on structural change of DNA

Peng

Liu

et al. 2021

Luminescence

View full text Add to dashboard Cite

In this work, a simple ratiometric method has been designed to detect Hg2+ based on the structural change between double‐stranded DNA (dsDNA) and its G‐quadruplex structure. When Hg2+ was added, the designed G‐quadruplex structure could change into the corresponding dsDNA by forming the T–Hg2+–T mismatch. This kind of variation resulted in a decrease in the fluorescence of the G‐quadruplex/N‐methyl mesoporphyrin IX (NMM) complex and an increase in the fluorescence from the dsDNA/SYBR Green I (SG I) pair. The secondary excitation wavelength of SG I was used to excite NMM and SG I simultaneously. The titration experiment indicated that the new method had a linear response within 0.7–2.5 μM Hg2+ with a limit of detection of 9.3 nM. Because using the T–Hg2+–T mismatch to recognize Hg2+ was very specific, the selectivity of the new method was also satisfactory. The recoveries ranged from 92.8% to 110.2% suggested that this new method could achieve a potential application for Hg2+ detection in real environmental samples.

show abstract

Section: Introductionmentioning

confidence: 99%

A label‐free ratiometric method to detect Hg²⁺ based on structural change of DNA

Peng

Liu

et al. 2021

Luminescence

View full text Add to dashboard Cite

show abstract

“…However, they usually require expensive and sophisticated instruments and skilled personnel, which hinder their wide application for onsite analysis of mercury contamination. Alternatively, considerable efforts have been focused on the development of molecular recognition probes for mercury detection based on small organic molecules, gold nanoparticles, DNAzymes, and proteins . However, a large number of small organic molecule-based assays show low water solubility, quench emissions, and poor selectivity .…”

Section: Introductionmentioning

confidence: 99%

Ratiometric-enhanced G-Quadruplex Probes for Amplified and Mix-to-Read Detection of Mercury Pollution in Aquatic Products

Yue

Deng

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

Mercury, as a global toxic pollutant, is easy to be accumulated in aquatic products and poses a great threat to human health. In this work, we proposed a mix-to-read, label-free, and robust assay for detecting mercury pollution in aquatic products by engineering a ratiometric-enhanced G-quadruplex probe. The transformation from the G-quadruplex to a hairpin-like structure allows us to confer a ratiometric and leveraged response to Hg 2+ , amplifying the signal-to-background ratio for Hg 2+ detection. Hg 2+ response was further improved by screening parallel-and antiparallel-, single-, and multiple-stranded G-quadruplex structures. Compared to the common aptamer probes, the ratiometric-enhanced G-quadruplex probe increased the sensitivity for Hg 2+ detection by 4.7 times. This proposed sensing system allowed a simple and one-tube homogenous detection of Hg 2+ at room temperature using a single unlabeled DNA sequence. Its application for Hg 2+ detection in fish and shrimp conferred satisfactory recovery rates ranging from 98.5 to 105.9%. The label-free and mix-to-read assay is promising for the onsite detection of mercury pollution and facilitating food safety of aquatic products.

show abstract

“…Although these methods provide satisfying detection performance, they require expensive instrumentation and skilled personnel to accomplish the operational procedures. To overcome this issue, several types of mercury-ion sensors have been developed based on small organic molecules, [11][12][13][14] gold nanoparticles, [15][16][17][18] aggregation-induced emission (AIE), [19][20][21] nanotubes, 22,23 electrochemical techniques, 7,24 DNAzymes 25 and proteins. 26 Many of these sensors show sufficient sensitivity and selectivity for the detection of mercury in aqueous solution; however, most of them still suffer from several limitations, such as low water solubility, sophisticated synthesis procedures, difficult modification of the probe materials and sample matrix interference.…”

Section: Introductionmentioning

confidence: 99%

A Label-free “Turn-on” Fluorescence Method for Detecting Mercury Ion in Aqueous Solution Based on N-Methyl Mesoporphyrin IX (NMM)/G-quadruplex DNA

Zhang

Ding

et al. 2017

ANAL. SCI.

View full text Add to dashboard Cite

In the present work, a simple design of "turn-on" fluorescence method for mercury ion was developed and explored based on specific T-Hg-T mismatches as a recognizer and N-methyl mesoporphyrin IX (NMM)/G-quadruplex DNA system as a reporter. The titration experiment showed that the mercury-ion concentration and the fluorescence intensity signal change exhibited a consistent linear correlation within the 50 to 500 nM range with a detection limit down to 12.9 nM. In a selectivity experiment, our method showed obvious selectivity against other metal ions, being consistent with other reported detection methods of mercury ion based on the specific reactivity of T-Hg-T mismatches. Our method was then successfully employed to detect mercury ion in pond water with excellent reliability.

show abstract

Rapid detection methodology for inorganic mercury (Hg2+) in seafood samples using conjugated polymer (1,4-bis-(8-(4-phenylthiazole-2-thiol)-octyloxy)-benzene) (PPT) by colorimetric and fluorescence spectroscopy

Cited by 16 publications

References 34 publications

A label‐free ratiometric method to detect Hg²⁺ based on structural change of DNA

A label‐free ratiometric method to detect Hg²⁺ based on structural change of DNA

Ratiometric-enhanced G-Quadruplex Probes for Amplified and Mix-to-Read Detection of Mercury Pollution in Aquatic Products

A Label-free “Turn-on” Fluorescence Method for Detecting Mercury Ion in Aqueous Solution Based on N-Methyl Mesoporphyrin IX (NMM)/G-quadruplex DNA

Contact Info

Product

Resources

About

Rapid detection methodology for inorganic mercury (Hg2+) in seafood samples using conjugated polymer (1,4-bis-(8-(4-phenylthiazole-2-thiol)-octyloxy)-benzene) (PPT) by colorimetric and fluorescence spectroscopy

Cited by 16 publications

References 34 publications

A label‐free ratiometric method to detect Hg2+ based on structural change of DNA

A label‐free ratiometric method to detect Hg2+ based on structural change of DNA

Ratiometric-enhanced G-Quadruplex Probes for Amplified and Mix-to-Read Detection of Mercury Pollution in Aquatic Products

A Label-free “Turn-on” Fluorescence Method for Detecting Mercury Ion in Aqueous Solution Based on N-Methyl Mesoporphyrin IX (NMM)/G-quadruplex DNA

Contact Info

Product

Resources

About

A label‐free ratiometric method to detect Hg²⁺ based on structural change of DNA

A label‐free ratiometric method to detect Hg²⁺ based on structural change of DNA