Rapid detection of 5 fungal diseases in sunflower (Helianthus annuus) using dual priming oligonucleotide system-based multiplex PCR and capillary electrophoresis

Qian, Yike; Wei, Shuang; Zhang, Na; Yang, Q.; Chen, Weimin; Zhang, Xiaoqin; Zhao, Huixia

doi:10.1016/j.slast.2022.03.004

Cited by 3 publications

(5 citation statements)

References 46 publications

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“…The concentration of 1 mM of methyl jasmonate outperformed all other concentrations in field emergence, with highest average of 80.83%, while the concentration of 3 mM recorded the lowest significant difference at 67.50%. This can be attributed to its initial superiority in the rate of initial field emergence as shown in Table (1). Previous research has shown that high concentrations of methyl jasmonate reduce the viability of maize seeds and hence seed germination by reducing the efficiency of amylase enzyme and ethylene production rate, both of which are necessary for seed germination, confirming the inverse relationship between methyl jasmonate concentrations and seed germination rate [13].…”

Section: Final Field Emergence Rate (%)mentioning

confidence: 54%

“…Additionally, the results indicate that different salinity levels negatively affected the fresh weight of seedlings, giving sunflower seeds at the salinity concentration of 8 ds m -1 the lowest average fresh weight at 0.767 g, while the salinity concentration of 4 ds m -1 gave the highest average at 3.249 g. This can be attributed to the low rate of initial and final germination, plumule length, and radicle length at the salinity concentration of 8 ds m -1 , as shown in Tables (1,2,3,4). This decrease may also be attributed to the hindrance of cambial activity in the root and stem, leading to a lack of increase in the thickness of new meristematic cells and their volume [18].…”

Section: Plant Fresh Weight (G)mentioning

confidence: 93%

“…Belonging to one of the largest plant families, the Asteraceae family, it is primarily cultivated for the extraction of oil used in cooking. Sunflower ranks fourth in oilseed production after palm oil, soybean, and rapeseed [1]. Its cultivation spans approximately 29 million hectares of arable land worldwide, covering 14% of the global demand for cooking oil [2].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Seed Priming with Methyl Jasmonate and Salinity of Irrigation Water on the Emergence and Activity of Sunflower Seedlings

Mahmoud,

Al-Fahad

2024

IOP Conf. Ser.: Earth Environ. Sci.

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The experiment was conducted in the field in the autumn season of 2023, in the fields of the College of Agriculture, Anbar University. To study the effect of methyl jasmonate concentrations and salinity of irrigation water on the germination characteristics of sunflower. The experiment was implemented using a randomized complete block design (RCBD) with factorial arrangement (two factors) and three replications. The experiment included two factors: the first factor consisted of three concentrations of methyl jasmonate (1, 2, 3 mM (mM)), and the second factor consisted of three concentrations of saline irrigation water (4, 6, 8 dS.m-1). The concentration of 1 (mM) exhibited superiority in the traits of initial germination percentage, final germination percentage, plumule length, radicale length, and fresh weight, achieving the highest averages in these traits at 61.7 %, 80.83 %, 9.54 cm, 6.16 cm, 3.26 g respectively. Conversely, the control treatment recorded the lowest rates at 32.5%, 69.17%, 6.15 cm, 2.93 cm, 0.99 g respectively. Regarding salinity levels, the concentration of 4 (dS.m-1) recorded the highest average in initial germination percentage, final germination percentage, plumule length, radicale length, and fresh and dry weights of seedlings at (45 %, 79.17 %, 10.27 cm, 5.43 cm, 3.24 g % respectively. In contrast, the 8 dS.m-1 salinity level recorded the lowest average at 29.2 %, 65%, 4.12 cm, 2.41cm, 0.76 g respectively.

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Section: Final Field Emergence Rate (%)mentioning

confidence: 54%

Section: Plant Fresh Weight (G)mentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Effect of Seed Priming with Methyl Jasmonate and Salinity of Irrigation Water on the Emergence and Activity of Sunflower Seedlings

Mahmoud,

Al-Fahad

2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…The mPCR molecular assays using more than one pair of primers are becoming increasingly popular, as they can detect multiple targets simultaneously in a single PCR [17]. The mPCR-based species identification methods have been developed for many organisms, including nematodes [29], fungi [64], bacteria [37], and viruses [28]. The development of mPCR in the present study provides rapid and costeffective means for simultaneous detection of the five mulberry bacterial wilt pathogens including P. ananatis, ECC, KpSC, KoC and R. pseudosolanacearum.…”

Section: Discussionmentioning

confidence: 99%

“…ananatis "Rp": R. pseudosolanacearum; "ECC": E. cloacae complex; "KpSC": K. pneumoniae species complex; "KoC": K. oxytoca complex. (58)(59)(60)(61)(62)(63)(64)(65)(66)(67)(68)(69). NC: the healthy mulberry genomic DNA as a negative control.…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of virulence-related genes by multiplex PCR in five pathogenic bacteria of mulberry bacterial wilt

Yuan,

Qazi,

Huang

et al. 2024

Chem. Biol. Technol. Agric.

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Mulberry bacterial wilt is a devastating disease that is difficult to control and causes serious economic losses to the sericulture industry. This disease is mostly caused by a diverse group of pathogenic and opportunistic bacteria including, Ralstonia pseudosolanacearum, Pantoea ananatis, Enterobacter cloacae complex (ECC), Klebsiella pneumoniae species complex (KpSC), and K. oxytoca complex (KoC). Due to the lack of a rapid and reliable test to simultaneously detect these complex pathogens of mulberry wilt, we developed a multiplex PCR (mPCR) assay to detect five virulence-related genes carried by the pathogenic bacteria of mulberry bacterial wilt disease. The primers were designed for the virulence-related genes: pleD (GGDF structural domain-containing protein), yjfP (esterase), pelY (peripheral pectate lyase), ampD (N-acetyl-anhydromuranmyl-L-alanine amidase), and ripW (type III effector). Overall, the developed mPCR assay showed highly specific, sensitive and reproducible detection of target pathogens. Briefly, the results showed that the mPCR was highly specific in individual reactions, and the lowest detection concentration of the five pathogenic bacteria was 1.87 × 103 CFU/mL (DNA = 2.45 pg/μL). From 46 natural mulberry wilt samples, the mPCR detection rates of P. ananatis, ECC, KpSC, KoC and R. pseudosolanacearum were 8.69, 91.3, 34.7, 23.9 and 65.21%, respectively. The traditional culture media isolation methods showed comparable results. The pathogenicity test of 84 suspected pathogenic bacteria revealed that the morbidity (average morbidity level) caused by the pathogenic bacteria detected by mPCR was ≥ 65.5%, while the morbidity of the undetected pathogenic bacteria was ≤ 35.5%. Based on these results, we believe that the mPCR developed in the present study will be useful in rapid, reproducible, and sensitive detection of the pathogenic bacteria causing mulberry bacterial wilt including, R. pseudosolanacearum, P. ananatis, ECC, KpSC, and KoC. Graphical abstract

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Emerging analytical techniques in food safety and biosecurity

Yahaya,

Yahya,

Yahya

et al. 2024

Green Chemistry in Food Analysis

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Rapid detection of 5 fungal diseases in sunflower (Helianthus annuus) using dual priming oligonucleotide system-based multiplex PCR and capillary electrophoresis

Cited by 3 publications

References 46 publications

Effect of Seed Priming with Methyl Jasmonate and Salinity of Irrigation Water on the Emergence and Activity of Sunflower Seedlings

Effect of Seed Priming with Methyl Jasmonate and Salinity of Irrigation Water on the Emergence and Activity of Sunflower Seedlings

Rapid detection of virulence-related genes by multiplex PCR in five pathogenic bacteria of mulberry bacterial wilt

Emerging analytical techniques in food safety and biosecurity

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