Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip

Kawano, Ryuji; Osaki, Toshihisa; Sasaki, Hirotaka; Takinoue, Masahiro; Yoshizawa, Satoko; Takeuchi, Shoji

doi:10.1021/ja2026085

Cited by 196 publications

(156 citation statements)

References 33 publications

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“…Various analytical techniques exist that can be used for molecular detection of pollutants 1,2 , explosives [3][4][5] , narcotics 6,7 , and pesticides 8 . Often "real-samples" contain a complex mixture of compounds, which need to be quantified.…”

Section: Introductionmentioning

confidence: 99%

Self-assembled nanoparticle arrays for multiphase trace analyte detection

et al. 2012

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Nanoplasmonic structures designed for trace analyte detection by surface enhanced Raman spectroscopy typically require sophisticated nanofabrication techniques. An alternative to fabricating such arrays is to rely on self-assembly of nanoparticles at liquid-liquid or liquid-air interfaces into close-packed arrays.The density of the arrays can be fine tuned by modifying the nanoparticle functionality, pH of the solution, and salt concentration. Importantly, these arrays are robust, "self-healing", reproducible, and extremely easy to handle. Herein, we report on the use of such platforms made of Au nanoparticles for detection of (multi)analytes from the aqueous, organic, or air phases. The total interfacial area of the Au array, at the liquid-liquid interface, is approximately 25 mm 2 , making this platform ideal for small volume samples, low concentrations, and trace analytes. Importantly, the ease of assembly and rapid 2 detection makes this platform ideal for in-field sample testing of toxins such as explosives, drugs, or other hazardous chemicals.3

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Section: Introductionmentioning

confidence: 99%

Self-assembled nanoparticle arrays for multiphase trace analyte detection

et al. 2012

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“…11 Using bilayer lipid membranes (BLMs), we have been measuring various membrane protein and biological nanopores, e.g., single molecule detection using DNA aptamers, 14 high-throughput ion channel measurements at the single-protein level, 11,15 solution-exchange experiment in the microdroplet system, 16 and the development of portable nanopore sensors. 17 In this paper, we report on the pore-forming properties of magainin 1 (GIGKFLHSAGKFGKAFVGEIMKS) from channel current conductance measurements using the high-throughput DCM.…”

Section: Introductionmentioning

confidence: 99%

Channel Current Analysis for Pore-forming Properties of an Antimicrobial Peptide, Magainin 1, Using the Droplet Contact Method

Watanabe

Kawano

2016

ANAL. SCI.

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This study describes the pore-forming properties of magainin 1 in planar lipid bilayers. These bilayers were prepared by the droplet contact method, which was executed on a microfabricated device for a high-throughput study. We arrayed four droplet chambers parallelly in the single device, and the current measurements were carried out simultaneously. Using this system, we measured the channel current conductance of magainin 1. We determined the pore size and the number of assembling monomers in magainin pores in mammalian and bacterial model membranes. This system is a powerful tool for analyzing transmembrane peptides and their antimicrobial activities.

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“…12 Furthermore, we constructed a droplet array for high-throughput measurements. 13 Using bilayer lipid membranes (BLMs), we measured various membrane proteins and biological nanopore, for example, single molecule detection using DNA aptamers, 14 high-throughput ion channel measurements at the single-protein level, 13,15,16 solutionexchange techniques via the microdroplet system, 17 and the development of portable nanopore sensors. 18 In this study, we Figure 1.…”

Section: Introductionmentioning

confidence: 99%

Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array

2016

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This paper describes a method of hairpin DNA (hpDNA) unzipping analysis using a biological nanopore array. Various lengths of hpDNAs were captured and translocated through the nanopore and the individual duration times were monitored. The correlation between the unzipping time and the simulated hybridization energy of the duplexes was able to be modeled as first-ordered reaction. This method is one of the approach for understanding of basic biological reactions in DNA replication and RNA transcription.

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Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip

Cited by 196 publications

References 33 publications

Self-assembled nanoparticle arrays for multiphase trace analyte detection

Self-assembled nanoparticle arrays for multiphase trace analyte detection

Channel Current Analysis for Pore-forming Properties of an Antimicrobial Peptide, Magainin 1, Using the Droplet Contact Method

Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array

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