Rapid detection of Aeromonas hydrophila with a DNAzyme-based sensor

Ma, Xiaoyi; wang, Cang; Qin, Mingcan; Tian, Xiaopeng; Fan, Shihui; Zu, Hangtian; Lyu, Mingsheng; Wang, Shujun

doi:10.1016/j.foodcont.2020.107829

Cited by 15 publications

(14 citation statements)

References 44 publications

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“…According to reports, the crude extracellular mixture of bacteria can be used as targets to screen highly specific DNAZzyme (Ali, Wolfe, et al., 2019; Fan et al., 2021; Gu et al., 2019; Ma et al., 2020). First, 500 μl of V. alginolyticus culture was inoculated into 50 ml of LB medium and cultured at 30°C and 180 rpm for 12 h until the OD 600 reached 0.8.…”

Section: Methodsmentioning

confidence: 99%

“…According to reports, the crude extracellular mixture of bacteria can be used as targets to screen highly specific DNAZzyme (Ali, Wolfe, et al, 2019;Fan et al, 2021;Gu et al, 2019;Ma et al, 2020).…”

Section: Alginolyticus Crude Extracellular Mixture (Cem-va) Preparationmentioning

confidence: 99%

“…The fluorescence signal was used as the detection index, the RNA‐cleaving fluorogenic DNAzyme was used to detect Vibrio anguillarum (LOD: 4 × 10 3 CFU/ml) (Gu et al., 2019), K lebsiella pneumoniae (LOD: 10 5 CFU/ml) (Ali et al., 2019). and Aeromonas hydrophila (LOD: 36 CFU/ml) (Ma et al., 2020). When the reaction time was extended to 72 h, the DNAzyme can detect 10 CFUs of Legionella pneumophila by gel‐based method (Rothenbroker et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

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Fast and sensitive graphene oxide‐DNAzyme‐based biosensor for Vibrio alginolyticus detection

Tian¹,

Hu²,

Wei³

et al. 2022

Journal of Fish Diseases

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DNAzymes have been widely and effectively used for the detection of pathogenic bacteria, which pose a serious public health threat. However, the rapid and cost-effective detection of such bacteria remains a major challenge. In this study, we successfully selected Vibrio alginolyticus-specific DNAzymes. The activity of the candidates was assessed via fluorescence intensity and gel electrophoresis. The DNAzyme DT1 had a detection limit of 31 CFU/ml for V. alginolyticus and exhibited high specificity.Graphene oxide (GO) was used to develop a DNAzyme-based fluorescent sensor for the detection of V. alginolyticus, which significantly improved detection performance and shortened the reaction time as little as 10 s. The proposed method was then validated using crab, shrimp, fish, clam, and oyster samples. This study thus provides a new method for the rapid and sensitive detection of V. alginolyticus.

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Section: Methodsmentioning

confidence: 99%

Section: Alginolyticus Crude Extracellular Mixture (Cem-va) Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fast and sensitive graphene oxide‐DNAzyme‐based biosensor for Vibrio alginolyticus detection

Tian¹,

Hu²,

Wei³

et al. 2022

Journal of Fish Diseases

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“…Specifically, sensors relying on fluorescence-based detection represent a growing body of work. For example, Ma et al developed a fluorescence-based biosensor for Aeromonas hydrophila a highly pathogenic bacteria that is often present within food products . The platform relied on a conventional RNA-cleaving fluorescent DNAzyme system in which target-induced cleavage of a fluorophore-quencher substrate by an A. hydrophila -responsive DNAzyme resulted in separation between the two entities, giving rise to a detectable fluorescence signal.…”

Section: Dnazyme Applications In Biosensingmentioning

confidence: 99%

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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Since their discovery almost three decades ago, DNAzymes have been used extensively in biosensing. Depending on the type of DNAzyme being used, these functional oligonucleotides can act as molecular recognition elements within biosensors, offering high specificity to their target analyte, or as reporters capable of transducing a detectable signal. Several parameters need to be considered when designing a DNAzyme-based biosensor. In particular, given that many of these biosensors immobilize DNAzymes onto a sensing surface, selecting an appropriate immobilization strategy is vital. Suboptimal immobilization can result in both DNAzyme detachment and poor accessibility toward the target, leading to low sensing accuracy and sensitivity. Various approaches have been employed for DNAzyme immobilization within biosensors, ranging from amine and thiol-based covalent attachment to non-covalent strategies involving biotin–streptavidin interactions, DNA hybridization, electrostatic interactions, and physical entrapment. While the properties of each strategy inform its applicability within a proposed sensor, the selection of an appropriate strategy is largely dependent on the desired application. This is especially true given the diverse use of DNAzyme-based biosensors for the detection of pathogens, metal ions, and clinical biomarkers. In an effort to make the development of such sensors easier to navigate, this paper provides a comprehensive review of existing immobilization strategies, with a focus on their respective advantages, drawbacks, and optimal conditions for use. Next, common applications of existing DNAzyme-based biosensors are discussed. Last, emerging and future trends in the development of DNAzyme-based biosensors are discussed, and gaps in existing research worthy of exploration are identified.

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“…Consequently, target detection in food samples was achieved with concentrations as low as 400 cells/mL. Moreover, Ma et al investigated the use of DNAzymes as biorecognition elements for the detection of Aeromonas hydrophila , which is a highly pathogenic bacteria posing human health threats with their presence in food and the environment [34] . An in vitro selection process had been carried out to select the used DNAzyme which exhibited the highest activity.…”

Section: Biosensors For the Detection Of Pathogensmentioning

confidence: 99%

Recent advances in the biosensors application for the detection of bacteria and viruses in wastewater

Kadadou¹,

Tizani²,

Wadi³

et al. 2022

Journal of Environmental Chemical Engineering

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Rapid detection of Aeromonas hydrophila with a DNAzyme-based sensor

Cited by 15 publications

References 44 publications

Fast and sensitive graphene oxide‐DNAzyme‐based biosensor for Vibrio alginolyticus detection

Fast and sensitive graphene oxide‐DNAzyme‐based biosensor for Vibrio alginolyticus detection

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

Recent advances in the biosensors application for the detection of bacteria and viruses in wastewater

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