Rapid Detection of Anabolic Steroids in Urine by Protein Arrays

Zhou, Yong; Du, Guanhua; Geng, Manman; Lu, Zheng

doi:10.1055/s-2005-865824

Cited by 5 publications

(5 citation statements)

References 16 publications

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“…870 The detection of antibiotics in complex environments has been reported using Raman spectroscopy, aptamer-based sensors, 871 and photophysical methods. 872 Luminescent sensors for opioids, 873 over-the-counter drugs, 874 and antibiotics 875 have also been reported.…”

Section: Pharmaceutical Agentsmentioning

confidence: 99%

Supramolecular Luminescent Sensors

2018

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There is great need for stand-alone luminescence-based chemosensors that exemplify selectivity, sensitivity, and applicability and that overcome the challenges that arise from complex, real-world media. Discussed herein are recent developments toward these goals in the field of supramolecular luminescent chemosensors, including macrocycles, polymers, and nanomaterials. Specific focus is placed on the development of new macrocycle hosts since 2010, coupled with considerations of the underlying principles of supramolecular chemistry as well as analytes of interest and common luminophores. State-of-the-art developments in the fields of polymer and nanomaterial sensors are also examined, and some remaining unsolved challenges in the area of chemosensors are discussed. CONTENTS 4.4. Explosives 4.5. Pharmaceutical Agents 4.6. Biologically Relevant 4.6.1. Adenosine Triphosphate (ATP)

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Section: Pharmaceutical Agentsmentioning

confidence: 99%

Supramolecular Luminescent Sensors

2018

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“…for example to re-evaluate athletes' urinary and serum hormones profile, and so forth). Furthermore, until new methods for testosterone misuse detection (41,43) in treated hypogonadal athletes are validated and approved by WADA, it is necessary to know the individual treatment schedules exactly and to worldwide standardize endocrinological monitoring programmes during authorized testosterone treatments. In this sense, further studies on the serum and urinary hormones profiles of hypogonadal athletes of different races treated with different testosterone formulations or doses are warranted.…”

Section: Serum Hormones Profilementioning

confidence: 99%

Urinary and serum hormones profiles after testosterone enanthate administration in male hypogonadism: Concerns on the detection of doping with testosterone in treated hypogonadal athletes

Luigi

Sgrò

Romanelli

et al. 2009

J Endocrinol Invest

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Whereas the observed prolonged hyperandrogenism partially limited data interpretation, the report ed characteristics of variation of urinary parameters might be used to suspect testosterone misuse in hypogonadal athletes treated with testosterone enanthate. In this sense, while the actual threshold for tes tos terone/epites tos ter one ratio was confirmed to be of reduced usefulness, we suggest a contemporary evaluation of whole urinary androgen metabolites profile and serum androgens, at specific time points after testosterone enanthate administration. Moreover, an adequate tailoring of treatment, to avoid transitory hyperandrogenism, is highly advisable. Further studies on strategies for detecting doping with testosterone in hypogonadal athletes are warranted.

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“…This surface was used successfully in multireceptor immobilization, including estrogen receptor-β, adrenergic receptor-β 2 , μ-opioid receptor, androgen receptor, and so forth, in prohibited drug detection. 15 To verify the receptor binding activity on the chip, we performed the traditional ligand binding saturation assay and a competitive assay using different strategies. As shown in Figure 3, the shape of the dose dependence curve showed a typical convex curve, which suggested a positive cooperative binding of labeled testosterone with the protein arrays.…”

Section: Discussionmentioning

confidence: 99%

“…Fang et al 14 verified the binding assay on the chip by membrane receptors. We have used a multiple-receptor chip to detect prohibited drugs in athletes' urine 15 and an enzyme chip to screen elastase inhibitors. 16 However, to our knowledge, there are no reports about the application of receptor chips in high-throughput drug discovery.…”

Section: Introductionmentioning

confidence: 99%

Using the Protein Chip to Screen Agonists and Antagonists of the Androgen Receptor

Zhou¹,

Liu²,

Wang³

et al. 2008

SLAS Discovery

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Based on its important physical and pathological function, the androgen receptor (AR) is regarded as a significant drug target. In this report, the authors describe a novel strategy of protein chip technology to screen agonists and antagonists of AR. First, the AR ligand binding domain (AR-LBD) was expressed in Escherichia coli, purified, and then immobilized on a silane-polysaccharide surface of a protein chip. Second, the affinities of methyltestosterone (MT) and fluorescent-labeled testosterone for the AR-LBD protein chip were determined. Third, a converse strategy of the protein chip was tested to evaluate its reliability as a drug screening method. Fourth, a 10,067-compound library was screened to find new ligands of AR. From the results, the K(d) of testosterone and the IC(50) of MT are consistent with the literature (0.61 vs. 0.49 nM 2.88 vs. 3.90 nM, respectively). The Z' factor of the high-throughput screening (HTS) method was 0.76, which meets the requirement of drug screening (>0.4). Finally, 3 active ligands of AR were identified with their IC( 50) values of 3.63, 2.19, and 1.71 microM, respectively. In summary, the novel strategy of the AR-LBD protein chip was suitable for HTS at the molecular level.

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Rapid Detection of Anabolic Steroids in Urine by Protein Arrays

Cited by 5 publications

References 16 publications

Supramolecular Luminescent Sensors

Supramolecular Luminescent Sensors

Urinary and serum hormones profiles after testosterone enanthate administration in male hypogonadism: Concerns on the detection of doping with testosterone in treated hypogonadal athletes

Using the Protein Chip to Screen Agonists and Antagonists of the Androgen Receptor

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