Rapid Detection of Anti-SARS-CoV-2 Antibody Using a Selenium Nanoparticle-Based Lateral Flow Immunoassay

Chen, Chunxia; Hu, Hangzhan; Li, Xiaoquan; Zheng, Zhi; Wang, Zhizeng; Wang, Xuance; Zheng, Peiming; Cui, Facai; Li, Gang; Wang, Yaohui; Liu, Zhigang; Ma, Yi

doi:10.1109/tnb.2021.3105662

Cited by 38 publications

(28 citation statements)

References 25 publications

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“…In contrast, the metal(loid)s content in vegetables, rice grain, and fish around a large Cu-smelter all exceeded the maximum permissible level of food in China [ 18 ]. In addition, the metal(loid)s contamination in soil-crop systems around the NSPI has also been reported in several other countries [ 19 , 20 ]. However, there is little information about the migration of metal(loid)s in the soil-wheat system around the NSPI in China.…”

Section: Introductionmentioning

confidence: 87%

Metal(loid)s Spatial Distribution, Accumulation, and Potential Health Risk Assessment in Soil-Wheat Systems near a Pb/Zn Smelter in Henan Province, Central China

Liu

Ren

et al. 2022

IJERPH

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To understand the influence of Pb/Zn smelter on surrounding environment, 110 soil and 62 wheat grain samples (62 paired samples) were collected nearby a Pb/Zn smelter in Jiaozuo City, Henan Province, China. The content and spatial distribution of metal(loid)s in the soil-wheat system, and the potential health risk via consumption of wheat grains were determined. Results showed that the average content of Pb, Cd, As, Cu, Zn, and Ni in soil were 129.16, 4.28, 17.95, 20.43, 79.36, and 9.42 mg/kg, respectively. The content of Cd in almost all soil samples (99.1%) exceeded the national limitation of China (0.6 mg/kg). Spatial distribution analysis indicated that atmospheric deposition might be the main pollution source of Pb, Cd, As, and Zn in soil. In addition, the average content of Pb, Cd, As, Cu, Zn, and Ni in wheat grain were 0.62, 0.35, 0.10, 3.7, 35.77, and 0.15 mg/kg, respectively, with the average Pb and Cd content exceeding the national limitation of China. The average bioaccumulation factor of these metal(loid)s followed the following order: Zn (0.507) > Cu (0.239) > Cd (0.134) > Ni (0.024) > Pb (0.007) > As (0.006). Health risk assessment indicated that the average noncarcinogenic risk of children (6.78) was much higher than that of adults (2.83), and the carcinogenic risk of almost all wheat grain is higher than the acceptable range, with an average value of 2.43 × 10−2. These results indicated that humans who regularly consume these wheat grains might have a serious risk of noncarcinogenic and carcinogenic diseases.

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Section: Introductionmentioning

confidence: 87%

Metal(loid)s Spatial Distribution, Accumulation, and Potential Health Risk Assessment in Soil-Wheat Systems near a Pb/Zn Smelter in Henan Province, Central China

Liu

Ren

et al. 2022

IJERPH

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“…In terms of the mechanism of action in modern medicine, it is directly absorbed into blood through oral mucosa, avoiding the first-pass effect of the drug, so it has the advantages of better efficacy in activating blood circulation and removing blood stasis, faster onset of action, and higher safety. It not only improves vascular permeability, dilates blood vessels, and increases cerebral blood flow, thereby lowering blood pressure, but also specifically antagonizes platelet-activating factor (PAF), protects the corresponding target organs, reduces blood viscosity, improves microvascular function, and plays an anti-AS role [ 20 , 21 ]. In traditional Chinese medicine, Ginkgo is a good medicine for promoting blood circulation and removing blood stasis, which has the effects of promoting blood circulation and removing blood stasis, dredging veins, and relaxing collaterals.…”

Section: Discussionmentioning

confidence: 99%

Observation on the Efficacy of Ginkgo Ketone Ester Drop Pill in Improving Hypertension Combined with Carotid Atherosclerotic Plaque

Zhang¹,

Ma²

2022

Emergency Medicine International

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Purpose. To observe and analyze the efficacy of Ginkgo ketone ester drop pill intervention in patients with hypertension combined with carotid atherosclerotic plaque. Methods. The subjects were 300 patients with hypertension complicated with carotid atherosclerotic plaque treated in our hospital from January 2019 to September 2021. The grouping was done by the random number table method and 300 patients were divided equally into 2 groups. One group was treated with Western medicine alone (clopidogrel sulfate tablets, phenyl amlodipine tablets, irbesartan tablets, and resorvastatin) as the Western medicine group (WM group, n = 150), and one group was added to this intervention with Ginkgo ketone ester drop pill as the Chinese medicine group (CM group, n = 150). The observation indexes were the improvement of blood pressure (systolic blood pressure (SBP) and diastolic blood pressure (DBP)), blood lipids (low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), and total cholesterol (TC)), vascular endothelial function (nitric oxide (NO) and endothelin-1 (ET-1)), inflammatory factors (C-reactive protein (CRP) and interleukin-6 (IL-6)), plaque (intimal medial thickness (IMT) of carotid artery and plaque area), and efficacy after intervention and adverse effects during intervention in both groups. Results. After intervention, SBP, DBP, LDL-C, TG, and TC levels were lower and HDL-C levels were higher in both groups than before intervention in the same group, and both CM groups improved significantly compared with the WM group ( P < 0.05 ). After intervention, NO levels were higher and ET-1 levels were lower in both groups than before the intervention in the same group, and both CM groups improved significantly compared with the WM group ( P < 0.05 ). After intervention, CRP and IL-6 levels were lower in both groups than before intervention in the same group, and both CM groups improved significantly compared with the WM group ( P < 0.05 ). After intervention, IMT and plaque area were lower in both groups than before intervention in the same group and both CM groups improved significantly compared with the WM group ( P < 0.05 ). The total effective number of the CM group was better than the WM group ( P < 0.05 ), and there was no significant difference in the adverse reactions number in both groups ( P > 0.05 ). Conclusions. The treatment of hypertension combined with carotid atherosclerotic plaque with Ginkgo ketone ester drop pill helps to improve the blood pressure, blood lipid, and vascular endothelial function of patients and helps to inhibit the inflammation level and atherosclerotic plaque of patients, with significant efficacy and no significant adverse effects in patients, which is worthy of clinical promotion.

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“…The assembly of the LFIA was described previously. 35 , 36 The structure and assembly of the LFIA strip are shown in Figure 1A . The sample pad, conjugate pad, nitrocellulose membrane (NC membrane), and absorbent pad were assembled on a PVC plate in turn.…”

Section: Methodsmentioning

confidence: 99%

Development of a Point-of-Care Test Based on Selenium Nanoparticles for Heart-Type Fatty Acid-Binding Proteins in Human Plasma and Blood

Wang¹,

Wu²,

Ma³

et al. 2022

IJN

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Purpose A rapid, convenient, cost-effective in-home test method for identifying heart-type fatty acid-binding protein (H-FABP) in plasma and blood by a lateral-flow immunoassay (LFIA) based on selenium nanoparticles (SeNPs) was developed. Methods SeNPs were synthesized by using L-ascorbic acid to reduce seleninic acid at room temperature and conjugated with an anti-H-FABP monoclonal antibody. The limit of detection, specificity, and stability were measured, and clinical samples were analyzed. Results The SeNPs were spherical with a diameter of 39.48 ± 3.72 nm and were conjugated successfully with an anti-H-FABP antibody, resulting in a total diameter of 46.52 ± 2.95 nm. The kit was designed for the determination of H-FABP in plasma specimens and whole blood specimens. The limit of detection was 1 ng/mL in plasma and blood, and the results could be determined within 10 min. No cross-reaction occurred with cardiac troponin I, creatine kinase-MB or myoglobin. The kits were stored at 40 °C for up to 30 days without significant loss of activity. The sensitivity was determined to be 100%, the specificity 96.67%, and the overall coincidence rate 97.83%. Conclusion This SeNP assay kit can conveniently, rapidly, and sensitively detect H-FABP in plasma or blood with a readout of a simple color change visible to the naked eye with no special device, and can be used as an auxiliary means for the early screening of AMI. Clinical Trial Registration Plasma and blood samples were used under approval from the Experimental Animal Ethics committee of the Joint National Laboratory for Antibody Drug Engineering, Henan University. The clinical trial registration number was HUSOM-2019-047.

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Rapid Detection of Anti-SARS-CoV-2 Antibody Using a Selenium Nanoparticle-Based Lateral Flow Immunoassay

Cited by 38 publications

References 25 publications

Metal(loid)s Spatial Distribution, Accumulation, and Potential Health Risk Assessment in Soil-Wheat Systems near a Pb/Zn Smelter in Henan Province, Central China

Metal(loid)s Spatial Distribution, Accumulation, and Potential Health Risk Assessment in Soil-Wheat Systems near a Pb/Zn Smelter in Henan Province, Central China

Observation on the Efficacy of Ginkgo Ketone Ester Drop Pill in Improving Hypertension Combined with Carotid Atherosclerotic Plaque

Development of a Point-of-Care Test Based on Selenium Nanoparticles for Heart-Type Fatty Acid-Binding Proteins in Human Plasma and Blood

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