Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

Rapid Identification of Pathogens Causing Bloodstream Infections by Raman Spectroscopy and Raman Tweezers

“…Since the laser beam is tightly focused, microbial cells cover most of the volume from which Raman spectra are acquired. In this way, the signal of the surrounding serum is suppressed ( 39 , 48 ). The averaged spectra of the analyzed species in the serum, together with the PCA results, are presented in Fig.…”

“…Raman spectroscopy belongs to optical methods with multiple applications in clinical medicine, such as measurements of inflammatory markers, including C-reactive protein (CRP) ( 20 , 21 ); measurements of blood and urine chemicals ( 22 ); measurements of blood coagulation ( 23 ); and potential cancer diagnostics, even from human body fluids ( 23 – 26 ). In microbiology, numerous studies have shown the possibility of identifying microbes ( 27 – 38 ) and their virulence factors, including antibiotic resistance ( 39 – 41 ) and biofilm formation ( 42 – 44 ), by Raman spectroscopy and its variations. Raman tweezers, a combination of Raman spectroscopy and optical tweezers, allowed single-cell analyses of microbes in more depth by describing metabolic changes ( 45 , 46 ).…”

Direction-controllable dual-optical conveyors based on optical tweezers

“…For the study of light-induced particle transport, Zemánek et al discussed in depth various light-induced mechanisms for the controlled transport of microscopic particles; these mechanisms rely on the direct transfer of momentum between the particles and the incident light waves, on the combination of optical forces with external forces of other nature, and on light-triggered phoretic motion [27]. Subsequently, they introduced applied directions for research in light-induced particle transport, such as optical printing [28], medical testing [29], optically levitated in vacuum [30]. In recent years, several types of combination of microfluidic and optical tweezers have been proposed [31,32].…”

Light pressure across all scales: editorial

“…al [31], related with the unconventional properties of the Belinfante's spin momentum. The integration of optical tweezers with Raman spectroscopy, Raman tweezers, has opened to important applications in the spectroscopic characterization of biomolecules and cells that are experimentally investigated by Foti et al [32] and Bernatova et al [33], respectively.…”