Rapid detection of Atopobium vaginae and association with organisms implicated in bacterial vaginosis

Trama, Jason P.; Pascal, Kristen E.; Zimmerman, Jessica; Self, Matthew J.; Mordechai, Eli; Adelson, Martin E.

doi:10.1016/j.mcp.2007.08.002

Cited by 20 publications

(14 citation statements)

References 23 publications

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“…However, not all healthy females have vaginal microbiota dominated by lactobacilli [5], and other lactic acid producers such as Atopobium vaginae, Leptotrichia and Megasphaera have also been suggested to assist in maintaining the acidity of the vagina, [30,31]. With BV considered a non-specific (predominantly anaerobic) polymicrobial infection and the identities of many of the vaginal microflora remaining elusive [32], determining the health of the vaginal ecosystem appears to be subjective.…”

Section: Resultsmentioning

confidence: 99%

“…However, recognising only Gardnerella vaginalis as the overt pathogen in BV would be inaccurate, especially as G. vaginalis has shown a high sensitivity (100%) but low specificity (49%) for BV by also being detected in the absence of BV [14,16,28,47]. Synergy between G. vaginalis and Atopobium vaginae has been proposed as they are often detected together in BV [32,48,49,50,51,52] and only rarely has A. vaginae been detected in the absence of G. vaginalis [53] or in health [54,55]. A. vaginae was first implicated in BV in 2004, since then several studies have emerged associating this anaerobe with >80% of BV cases [54,56,57,58,59,60,61].…”

Section: Resultsmentioning

confidence: 99%

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Anaerobes and Bacterial Vaginosis in Pregnancy: Virulence Factors Contributing to Vaginal Colonisation

Africa

Nel

Stemmet

2014

IJERPH

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The aetiology and pathogenesis of bacterial vaginosis (BV) is unclear but it appears to be associated with factors that disrupt the normal acidity of the vagina thus altering the equilibrium between the normal vaginal microbiota. BV has serious implications for female morbidity, including reports of pelvic inflammatory disease, adverse pregnancy outcomes, increased susceptibility to sexually transmitted infections and infertility. This paper reviewed new available information regarding possible factors contributing to the establishment of the BV vaginal biofilm, examined the proposed role of anaerobic microbial species recently detected by new culture-independent methods and discusses developments related to the effects of BV on human pregnancy. The literature search included Pubmed (NLM), LISTA (EBSCO), and Web of Science. Because of the complexity and diversity of population groups, diagnosis and methodology used, no meta-analysis was performed. Several anaerobic microbial species previously missed in the laboratory diagnosis of BV have been revealed while taking cognisance of newly proposed theories of infection, thereby improving our understanding and knowledge of the complex aetiology and pathogenesis of BV and its perceived role in adverse pregnancy outcomes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Anaerobes and Bacterial Vaginosis in Pregnancy: Virulence Factors Contributing to Vaginal Colonisation

Africa

Nel

Stemmet

2014

IJERPH

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“…Bacterial vaginosis (BV) is a common aberrant condition associated with depletion of lactobacilli, elevated vaginal pH, and overgrowth of Atopobium, Mobiluncus, Prevotella, Gardnerella, and Megasphaera species (1,8,17).…”

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confidence: 99%

Analysis of Vaginal Lactobacilli from Healthy and Infected Brazilian Women

Martinez

Franceschini

Patta

et al. 2008

Appl Environ Microbiol

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Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H 2 O 2 -producing lactobacilli were not associated with protection against VVC.

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“…A study by Bradshaw et al (2006) supports a synergism between G. vaginalis and A. vaginae. Several researchers suggested that infection with A. vaginae is even more specific (and a diagnostically more valuable marker) for BV than infection with G. vaginalis (Bradshaw et al, 2006;Trama et al, 2008). These two species are strongly associated with bacterial biofilms (Swidsinski et al, 2005).…”

Section: Pathogenesis Of Bacterial Vaginosismentioning

confidence: 99%

“…(Goffinet et al, 2003). Cultures of bacteria, such as G. vaginalis are of no value for BV diagnosis as women who are merely colonized with this bacterium will also have positive cultures, whereas other bacteria, such as A. vaginae are fastidious, which makes cultivation difficult (Donders, 2007;Trama et al, 2008). Molecular detection methods may be more expensive than the gold standard but allow better characterization of the vaginal flora by targeting genes (mainly the 16S rRNA sequence) of specific bacterial genera or species (Fredricks et al, 2007).…”

Section: Culture and Pcr Detection Of Bacteria Associated With Bactermentioning

confidence: 99%

Normal flora and bacterial vaginosis in pregnancy: an overview

Redelinghuys

Ehlers

Dreyer

et al. 2015

Critical Reviews in Microbiology

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The female genital tract is an intricate, yet balanced ecosystem that hosts a variety of different residential microflora. The physiological changes that occur during pregnancy may disrupt this balanced ecosystem and predispose women to a potentially pathogenic microbiota. Bacteria that are associated with bacterial vaginosis (BV) are opportunistic pathogens that frequently form part of this microbiota. The overgrowth of and infections with these bacteria are linked to poor obstetric outcomes and increased transmission of other reproductive tract infections (RTIs). These infections increase women's susceptibility of acquiring HIV, the rates of HIV shedding and the development of Acquired Immune

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Rapid detection of Atopobium vaginae and association with organisms implicated in bacterial vaginosis

Cited by 20 publications

References 23 publications

Anaerobes and Bacterial Vaginosis in Pregnancy: Virulence Factors Contributing to Vaginal Colonisation

Anaerobes and Bacterial Vaginosis in Pregnancy: Virulence Factors Contributing to Vaginal Colonisation

Analysis of Vaginal Lactobacilli from Healthy and Infected Brazilian Women

Normal flora and bacterial vaginosis in pregnancy: an overview

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