Rapid detection of avian leukosis virus using a fluorescent microsphere immunochromatographic test strip assay

Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice

“…We conjugated fluorescent microspheres to mAb 2F7 (Wang et al, 2019). First, 400 µL of 0.05 M BB (pH = 8.0) in a 2-mL tube was mixed for 1 min with 100 µL of fluorescent microspheres.…”

Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay

“…Fluorescent microspheres on the modified carboxylate group should be used at approximately pH 5.0 (Majdinasab et al, 2015). The anti-OTA mAb was coupled to the FMs according to the carboxyl activation coupling method (Wang et al, 2019). Briefly, 100 µL of FMs were suspended in 400 ul of 0.05 M BB (pH 8.0).…”

“…The combination of FM and immunochromatographic test strip (ICTS) (FM-ICTS) and a fluorescence analyzer make it possible to quantitatively read and analyze fluorescence signals and avoids errors caused by visual observation. Furthermore, the concentration of analyte can be calculated by establishing a fitting curve (Wang et al, 2019).…”

Development of a colloidal gold immunochromatographic strip for rapid detection of avian coronavirus infectious bronchitis virus

“…The colloidal gold-labeled antibody-based immunochromatographic strip is rapid, easy to operate, low cost, is not dependent on professional equipment, and is widely used in poultry disease detection ( Li et al, 2013 ; Qian et al, 2015 ; Yu et al, 2018 ; Wang et al, 2019 ; Yu et al, 2020 ), as well as in certain other fields ( Zhao et al, 2008 ; Li et al, 2012 ; Meng et al, 2014 ; Li et al, 2020 ; Lee et al, 2021 ; Li et al, 2021 ; Guo et al, 2023 ). Zhang et al, (2010) reported the use of IBV colloidal gold immunochromatography for the first time, but their method was only used to detect the antibodies against IBV.…”