Rapid detection of bacterial cells by light scattering method

Abstract

Method of rapid detection of bacterial cells by light scattering is described. Determination of quantitative changes of bacteria is the given method based on the changes of their size distributing in the process of cultivation. Liquid medial are diluted and analyzed by the proposed technology to determine presence of bacteria. A method includes sounding of flow suspended bacterial cells by monochromatic coherent light, registration of signals of co-operation of sounding radiation with the explored microbiological objects by detects amplitudes and durations of scattered light impulses. Distribution of particles by sizes is determined from the measured functional dependence of number of registered particles from amplitude and duration of the proper electric impulses on the output photoreceiver. Detection is done for a range of particle size from 0.1 to 10 mkm, and thus particle's size distribution is determined. The results of studying of rapid detection of Escherichia coli by light scattering are described.

Light-scattering properties of microorganisms Desulfuromonas acetoxidans by influence of silver

“…Bacterial growth commonly can be investigated by the registration of bacterial suspension turbidity at λ = 410 nm. The distribution of particles in size is by the light scattering methods is proposed in (Bilyy et al, 2008;2011). It includes the sounding of flow suspended bacterial cells by monochromatic coherent light, the registration of cooperative signals of sounding radiation and the explored microbiological objects by detecting of amplitudes and duration of cattered light impulses.…”