Rapid Detection of Bloodstream Pathogens in Liver Transplantation Patients With FilmArray Multiplex Polymerase Chain Reaction Assays: Comparison With Conventional Methods

Otlu, Barış; Bayındır, Yaşar; Özdemir, Fatih; Ince, Volkan; Cuglan, S.; Hopoglu, M.; Yakupoğulları, Yusuf; Kizilkaya, C.; Kuzucu, Çiğdem; Işık, Burak; Yilmaz, Sezai

doi:10.1016/j.transproceed.2015.02.025

Cited by 9 publications

(6 citation statements)

References 25 publications

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“…In this study, all the samples are from blood cultures containing carbon powder, the accuracy of detection of the Filmarray was good comparing with the current clinical combination method, and this was consistent with the outcomes of previous researchers using non-carbon powder blood culture bottles. It is capable of providing rapid [18][19][20] and reliable results in the detection of pathogens present in automated blood culture systems [11,21], and it can be applied to the special patients [22][23][24] and the sterile body fluids, such as cerebrospinal, joint, pleural and ascitic fluids, bronchoscopy samples and abscesses [25][26][27]. The panel can not only save the patient cost ($30,000 saved per 100 patients tested) but also improve the ASP [28][29][30].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Performance of Filmarray Blood Culture Identification Panel on Detecting Blood Cultures Containing Activated Carbon Powder

Chen

Wang

2021

Preprint

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Objective: The FilmArray Blood Culture Identification (BCID) panel is a rapid microfluidic PCR amplification microbial detection system. Several studies have evaluated its clinical performance on the basis of blood culture bottles containing resins. However, proportion of hospitals in China use bottles with carbon power, which the performance of FilmArray has not been fully investigated. Therefore, this study is conducted to explore the accuracy of the panel using blood culture bottles with carbon power. Method: 147 venous blood cultures containing carbon powder were used to assess the microbial and antibiotic resistance detection ability of the FilmArray panel. Outcomes were compared with results of the clinical combination method and their consistency was analyzed. Results: FilmArray detected single microorganism in 121 samples, multiple microorganism in 9 cases and the consistency rate between the two methods was 90.6%. Among the 150 microorganisms detected, 85.1% (40/47) of staphylococcus contained the antibiotic resistant mecA gene, 15.3% (9/59) of Enterobacter detected the KPC gene, 7.7% (1/13) of Enterococcus has the vanA gene and the consistency with their clinical drug-resistant phenotypes were 93.6%, 86.4% and 100%, respectively. Conclusion: The identification rate of the FilmArray BCID panel using venous blood cultures with activated carbon powder was highly consistent with the outcomes of previous researchers using non-carbon powder blood culture bottles. It is capable of providing rapid and reliable results in the detection of pathogens present in automated blood culture systems.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Performance of Filmarray Blood Culture Identification Panel on Detecting Blood Cultures Containing Activated Carbon Powder

Chen

Wang

2021

Preprint

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“…This assay can be useful in quickly detecting common pathogens for which a given patient is at risk. In a study of liver transplant patients, a multiplex FilmArray did not miss infections detected by conventional methods [87]. Clinically important fungi not included on the panel were accordingly missed in multi-center trials comparing this assay to conventional diagnostic methods [88,89].…”

Section: Culture Independent Assaysmentioning

confidence: 94%

An Approach to Fungal Diagnostics in Solid Organ Transplantation

Gaston¹,

Azar²

2018

obm transplant

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The approach to diagnosing fungal infections following solid organ transplantation (SOT) is patient-specific. An assessment of an individual's risk for particular infections, also understood as the pre-test probability, should guide appropriate diagnostic testing whereas diagnostic stewardship is needed to produce interpretable, actionable and cost-sensitive results. This review provides a cognitive framework for practitioners aiming to diagnose fungal infections in recipients of SOT, taking into consideration the benefits and limitations of currently available diagnostic platforms. In order to better characterize diagnostic utility for this population, we focus on clinical studies that include patients with SOT in full or in part. The goal of this review is to improve the diagnosis of fungal infections in patients with SOT by encouraging a thoughtful, systematic diagnostic approach.

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“…FP, false positive; TP, true positive; Table 1. Continued [29,37,[46][47][48], two studies were labelled 'high-risk' for index tests [36,46], three studies were labelled 'unclear' for index tests [29,38,49], three studies were labelled 'unclear' for reference standards [29,50,51], two studies were labelled 'high-risk' for flow and timing [29,48,52] and six studies were labelled 'unclear' for flow and timing [37,46,49,51,[53][54][55]. Overall, all of the studies had low concern regarding applicability.…”

Section: Quality Assessmentmentioning

confidence: 99%

Diagnostic efficiency of the FilmArray blood culture identification (BCID) panel: a systematic review and meta-analysis

Yang,

Tao

2023

Journal of Medical Microbiology

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Introduction. The FilmArray blood culture identification panel (BCID) panel is a multiplex PCR assay with high sensitivity and specificity to identify the most common pathogens in bloodstream infections (BSIs). Hypothesis. We hypothesize that the BCID panel has good diagnostic performance for BSIs and can be popularized in clinical application. Aim: To provide summarized evidence for the diagnostic accuracy of the BCID panel for the identification of positive blood cultures. Methodology. We searched the MEDLINE, EMBASE and Cochrane databases through March 2021 and assessed the efficacy of the diagnostic test of the BCID panel. We performed a meta-analysis and calculated the summary sensitivity and specificity of the BCID panel. Systematic review protocols were registered in the International Prospective Register of Systematic Reviews (PROSPERO) (registration number CRD42021239176). Results. A total of 16 full-text articles were eligible for analysis. The overall sensitivities of the BCID panel on Gram-positive bacteria, Gram-negative bacteria and fungi were 97 % (95 % CI, 0.96–0.98), 100 % (95 % CI, 0.98–01.00) and 99 % (95 % CI, 0.87–1.00), respectively. The pooled diagnostic specificities were 99 % (95 % CI, 0.97–1.00), 100 % (95 % CI, 1.00–1.00) and 100 % (95 % CI, 1.00–1.00) for Gram-positive bacteria, Gram-negative bacteria and fungi, respectively. Conclusions. The BCID panel has high rule-in value for the early detection of BSI patients. The BCID panel can still provide valuable information for ruling out bacteremia or fungemia in populations with low pretest probability.

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Rapid Detection of Bloodstream Pathogens in Liver Transplantation Patients With FilmArray Multiplex Polymerase Chain Reaction Assays: Comparison With Conventional Methods

Cited by 9 publications

References 25 publications

Evaluation of the Performance of Filmarray Blood Culture Identification Panel on Detecting Blood Cultures Containing Activated Carbon Powder

Evaluation of the Performance of Filmarray Blood Culture Identification Panel on Detecting Blood Cultures Containing Activated Carbon Powder

An Approach to Fungal Diagnostics in Solid Organ Transplantation

Diagnostic efficiency of the FilmArray blood culture identification (BCID) panel: a systematic review and meta-analysis

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