Rapid detection of Brucella spp. by the loop-mediated isothermal amplification method

Ohtsuki, Ryuji; Kawamoto, Keiko; Kato, Yuto; Shah, Meena; Ezaki, Takayuki; Si, Makino

doi:10.1111/j.1365-2672.2008.03732.x

Cited by 60 publications

(42 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This new detection method could be helpful for high-throughput DNA detection in basic research and pointof-care testing. Several studies have used LAMP assays for detecting various pathogens, but mostly using a real-time turbidimeter (Parida et al, 2007;Yoneyama et al, 2007;Toriniwa et al, 2006;Thekisoe et al, 2010), a real-time PCR system (Cai et al, 2008;Ohtsuki et al, 2008) or 14 fluorescent detection reagents (FDR) (Eiken) (Adams et al, 2010), which require a UV illuminator for LAMP product discrimination. The use of expensive and specialized equipment reduces the versatility of LAMP and greatly limits the wide use of this technique, especially in developing countries.…”

Section: Discussionmentioning

confidence: 99%

Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection

et al. 2014

View full text Add to dashboard Cite

Entomological monitoring of Leishmania infection in leishmaniasis endemic areas offers epidemiologic advantages for predicting the risk and expansion of the disease, as well as evaluation of the effectiveness of control programs. In this study, we developed a highly sensitive loop-mediated isothermal amplification (LAMP) method for the mass screening of sand flies for Leishmania infection based on the 18S rRNA gene. The LAMP technique could detect 0.01 parasites, which was more sensitive than classical PCR. The method was robust and could amplify the target DNA within 1 hr from a crude sand fly template without DNA purification. Amplicon

show abstract

Section: Discussionmentioning

confidence: 99%

Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection

et al. 2014

View full text Add to dashboard Cite

show abstract

“…LAMP for the Brucella was developed against Brucella cell surface protein (bcsp)31 gene and omp25 gene and sensitivity of both LAMP assay was higher than PCR (Ohtsuki et al, 2008;Lin et al, 2011). LAMP with visual detection based on calcein has also been developed targeting the same omp25 (Pan et al, 2011).…”

Section: Serological Tests Like Rose Bengal Plate Test (Rbpt) Standamentioning

confidence: 99%

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Karthik¹,

Prabakar²,

Bharathi³

et al. 2016

JEBAS

View full text Add to dashboard Cite

KEYWORDS Brucellosis Equine B. abortus Poll evil Fistulous withers LAMP ABSTRACTBrucellosis is one of the major zoonotic diseases that affect several domestic animals, wild animals and also marine mammals. Though there is no specific Brucella sp. that can affect horses, B. abortus and B. suis can affect horses naturally and B. canis experimental infection has also been reported in equines. Brucellosis in equines is characterized by two conditions namely Poll evil and fistulous withers. Organism has its predilection for joints, ligaments and tendons in case of equines and causes inflammatory conditions leading to formation of fistula. Equine brucellosis has been documented from several parts of the world and prevalence has been reported time to time mostly based on serological diagnosis. Diagnosis of brucellosis mainly depends on serological methods though isolation of the organism is the gold standard. Due to hazardous nature of the pathogen, tests like Rose Bengal plate agglutination test, Standard tube agglutination test and other serological assays are commonly employed. Isothermal amplification assay like LAMP are gaining momentum these years due to swiftness in diagnosis of the pathogen. LAMP with high specificity and sensitivity for detection of Brucella spp. and also B. abortus has been developed in the recent years. Prevention and control of brucellosis is of utmost important to halt the spread of the organism to other animals and human. Trauma is a major reason for predisposition of poll evil and fistulous withers hence proper fitting of saddle will help to prevent the disease. Housing and feeding the horses separately can prevent spread of disease from cattle. The present review discusses equine brucellosis, its epidemiology, pathogenesis, clinical signs along with appropriate prevention and control strategies to be adapted.

show abstract

“…The clinical presentation of brucellosis is nonspecific and show great variability (Ohtsuki et al, 2008). Diagnosis of this disease, therefore, requires microbiological confirmation by means of the isolation of the bacteria or demonstration by serological tests for the presence of specific antibodies.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Brucellosis among the Hospital Patients of Peshawar, Khyber Pakhtunkhwa

Shahid¹

2014

J. Inf. Mol. Biol.

View full text Add to dashboard Cite

Rapid detection of Brucella spp. by the loop-mediated isothermal amplification method

Cited by 60 publications

References 37 publications

Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection

Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection

Equine brucellosis: Review on epidemiology, pathogenesis, clinical signs, prevention and control

Prevalence of Brucellosis among the Hospital Patients of Peshawar, Khyber Pakhtunkhwa

Contact Info

Product

Resources

About