Rapid detection of columnaris disease in channel catfish (Ictalurus punctatus) with a new species-specific 16-S rRNA gene-based PCR primer for Flavobacterium columnare

Bader, Joel A.; Shoemaker, Craig A.; Klesius, Phillip H.

doi:10.1016/s0167-7012(02)00208-7

Cited by 75 publications

(66 citation statements)

References 22 publications

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“…The fish were euthanized, and internal organs and muscle tissue were removed. Total DNA was isolated using the DNAeasy kit (Quiagen) and used as template for the PCR-based F. columnare detection methods of Toyama et al (1996) and Bader et al (2003a) with the following modification: rTaq polymerase was used for PCR (Takara Bio). PCR products were run on a 0.7% agarose gel and stained with Syber Green.…”

Section: Zebra Fish Carementioning

confidence: 99%

Susceptibility of zebra fish Danio rerio to infection by Flavobacterium columnare and F. johnsoniae

Moyer¹,

Hunnicutt²

2007

Dis. Aquat. Org.

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Section: Zebra Fish Carementioning

confidence: 99%

Susceptibility of zebra fish Danio rerio to infection by Flavobacterium columnare and F. johnsoniae

Moyer¹,

Hunnicutt²

2007

Dis. Aquat. Org.

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“…Bacterial disease outbreaks impose a significant constraint on the production of fish and shellfish (Bachère et al, 1995;Verschuere et al, 2000;Gomez et al, 2007). Bacterial pathogens have been reported to cause heavy mortality in both cultured and wild fish species in different parts of the world (Joseph and Clerk, 2002;ISSN : 0974-9411 (Print), 2231-5209 (Online) All Rights Reserved © Applied and Natural Science Foundation www.ansfoundation.org Bader et al, 2003;). The facultative bacterial pathogens prove a potential threat, when fish are under environmental and physiological stress (Kirjusina et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of antibacterial activity of two different earthworm species, Perionyx excavatus and Pheretima posthuma against pathogenic bacteria

Bansal

Gupta

Singh

et al. 2015

JANS

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Disease outbreaks are being increasingly recognized as a significant constraint on aquaculture production and trade affecting the economic development of the sector in many countries. Extracting and using biologically active compounds from earthworms has traditionally been practiced by indigenous people throughout the world. The aim of the present study was to shown antimicrobial activity through earthworm extract against fish bacterial pathogens. In total, 8 bacterial strains i.e. 6 gram negative viz. Aeromonas hydrophila, Pseudomonas aeruginosa, P. fluorescens, E.coli, Enterobacter aerogens and Shigella sp. and 2 gram positive viz. Staphylococcus aureus and Micrococcus luteus were identified. The extract of earthworm Perionyx excavatus, Pheretima posthuma were prepared and antimicrobial activity of the extract was determined by antimicrobial well diffusion assay. After 24 hrs of incubation period, it was observed that earthworm extract showed antibacterial activity against isolated bacterial strains. Among earthworm extract of two different species, the maximum zone of inhibition was shown against A. hydrophila by Perionyx excavatus (18.33± 0.66 mm) and P. posthuma (16.66±0.33). P. excavatus showed antibacterial activity against all pathogenic bacteria except Shigella spp. However on the other hand, P.posthuma showed antibacterial activity against A. hydrophila, P. fluorescens, E.coli, and S. aureus. The study has proved that earthworm extract can be effectively used for suppression of bacterial infection in fishes and that it can used as potential antimicrobial drug against commercial antibiotic resistance bacteria.

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“…The ubiquitous distribution of the organism in fresh water environments and the tendency for fish to acquire the disease after mechanical and/or environmental insults makes F. columnare among the most described pathogens in cultured, ornamental and wild fish populations (Triyanto and Wakabayashi, 1999;Austin and Austin, 1999;Bader et al, 2003;Jinu and Goodwin, 2004). Epizootics of columnaris are rarely spontaneous and have been troublesome in hatcheries, pen, cage culture and in pond culture, particularly under adverse environmental conditions and factors stressful to the fish.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Flavobacterium columnare (Bernardet et al. 2002) from four tropical fish species in Brazil

Pilarski

Rossini

Ceccarelli³

2008

Braz. J. Biol.

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Flavobacterium columnare is the causative agent of columnaris disease in freshwater fish, implicated in skin and gill disease, often causing high mortality. The aim of this study was the isolation and characterization of Flavobacterium columnare in tropical fish in Brazil. Piracanjuba (Brycon orbignyanus), pacu (Piaractus mesopotamicus), tambaqui (Colossoma macropomum) and cascudo (Hypostomus plecostomus) were examined for external lesions showing signs of colunmaris disease such as greyish white spots, especially on the head, dorsal part and caudal fin of the fish. The sampling comprised 50 samples representing four different fish species selected for study. Samples for culture were obtained by skin and kidney scrapes with a sterile cotton swabs of columnaris disease fish and streaked onto Carlson and Pacha (1968) artificial culture medium (broth and solid) which were used for isolation. The strains in the liquid medium were Gram negative, long, filamentous, exhibited flexing movements (gliding motility), contained a large number of long slender bacteria and gathered into 'columns'. Strains on the agar produced yellow-pale colonies, rather small, flat that had rhizoid edges. A total of four Flavobacterium columnare were isolated: 01 Brycon orbignyanus strain, 01 Piaractus mesopotamicus strain, 01 Colossoma macropomum strain, and 01 Hypostomus plecostomus strain. Biochemical characterization, with its absorption of Congo red dye, production of flexirubin-type pigments, H 2 S production and reduction of nitrates proved that the isolate could be classified as Flavobacterium columnare. Keywords

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Rapid detection of columnaris disease in channel catfish (Ictalurus punctatus) with a new species-specific 16-S rRNA gene-based PCR primer for Flavobacterium columnare

Cited by 75 publications

References 22 publications

Susceptibility of zebra fish Danio rerio to infection by Flavobacterium columnare and F. johnsoniae

Susceptibility of zebra fish Danio rerio to infection by Flavobacterium columnare and F. johnsoniae

Comparative study of antibacterial activity of two different earthworm species, Perionyx excavatus and Pheretima posthuma against pathogenic bacteria

Isolation and characterization of Flavobacterium columnare (Bernardet et al. 2002) from four tropical fish species in Brazil

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