Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay

Abstract

Cysticercosis is a neglected tropical disease caused by the larvae of Taenia solium in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of Cysticercus cellulosae. UCP particles were bound to two antigens, TSOL18 and GP50; samples were captured, and the signal from the UCP particles was converted into a detectable signal for analysis using a biosensor. Compared to ELISA, UPT-LF has higher sensitivity and specificity, with a sensitivity of 93.59% and 97.44%, respectively, in the case of TSOL18 and GP50 antigens and a specificity of 100% for both. Given its rapidness, small volume, high sensitivity and specificity, and good stability and reproducibility, this method could be used in the diagnosis of cysticercosis.

Development and evaluation of time-resolved rapid immunofluorescence test for detection of TSOL18 specific antibody in porcine cysticercosis infections

“…The sensitivity of the ICS method exhibits 1% increase when compared to the LFA method [ 22 ]. Not only does ICS demonstrate exceptional detection results, but it also offers a more cost-effective alternative to LFA.…”

“…The same batch of negative (10) and positive serums (10) was tested at different time points and two temperatures (4 and 25 ℃) and each test had three replicates. The results at different time points were compared with the initial results to calculate the relative deviation: a relative deviation of less than 15% was accepted [ 22 ].…”

“…Pigs attained a nearly complete immune protection rate of 99.5% when they were immunized solely with the recombinant TSOL18 antigen [ 21 ]. A research used TSOL18 to develop a method for detecting porcine cysticercosis based on a lateral-flow assay (LFA) that uses up-converting phosphor technology [ 22 ]. The sensitivity of this method was 93.59% and the specificity was 100% [ 22 ].…”

“…A research used TSOL18 to develop a method for detecting porcine cysticercosis based on a lateral-flow assay (LFA) that uses up-converting phosphor technology [ 22 ]. The sensitivity of this method was 93.59% and the specificity was 100% [ 22 ]. In this study, we aimed to assess a simple, rapid, and stable TRFM-ICS using TSOL18 antigen to achieve large-scale and rapid field detection of Cysticercus cellulose .…”

Lateral Flow Immunoassay Based on Time-Resolved Fluorescence Microspheres for Rapid and Quantitative Screening CA199 in Human Serum

“…However, the traditional AuNPs-based LFIA has limitations in high-sensitivity quantification. Varied novel nanoparticles, such as quantum dots [ 22 ], fluorescent microspheres [ 23 ], magnetic nanoparticles [ 24 ], and up-conversion phosphorescent nanoparticles [ 25 ], have been used as reporters in LFIA to break through the limitation. Among them, fluorescence microspheres suffer from narrow Stokes shift, strong background signals, and photobleaching, influencing the accuracy and sensitivity of detection results [ 26 ].…”