Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH Method: a multicenter investigation

Evaluation of Three Rapid Diagnostic Methods for Direct Identification of Microorganisms in Positive Blood Cultures

“…More recently, the Enterococcus QuickFISH assay was shown to be 97% sensitive for the detection of non-E. faecalis enterococci (38). Our results are therefore similar to those of previous studies, with observed sensitivity and specificity of 98.3% and 100%, respectively, for the identification of staphylococci and 100% sensitivity and specificity for the detection of Enterococcus species in monomicrobial cultures (data not shown).…”

“…The methods evaluated in our study are associated with differing hands-on time requirements as well as reagent and capital equipment costs, but all three offer the potential to substantially reduce the time to a result compared with routine methods (18,38,48). Nevertheless, implementation of rapid blood culture identification in a clinical microbiology laboratory will likely not eliminate the need for additional testing to be performed upon recovery of the microorganisms present on subculture.…”

Evaluation of real-time PCR and pyrosequencing for screening incubating blood culture bottles from adults with suspected bloodstream infection

“…Several molecular-based platforms including real-time PCR [19, 20], PNA FISH [21, 22], Verigene (Nanosphere Inc., Northbrook, IL) [23], FilmArray (BioFire, Salt Lake City, UT) [24] and MALDI-TOF MS [25, 26] have been used to successfully detect and identify microorganisms associated with BSI, however these assays all require fluid from positive blood culture bottles as starting material. In contrast, this study illustrated our success in combining real-time PCR and Pyrosequencing to detect and identify bacteria in incubating blood culture fluid prior to growth being detected in culture.…”

Artificial Nucleic Acid Probes and Their Applications in Clinical Microbiology

“…Laboratory evaluation of their Staphylococcus QuickFISH test demonstrated a high specificity (approximately 99%) for both S. aureus and coagulasenegative Staphylococci individually and 90% when both are present together (Deck et al, 2012). Similarly, their Yeast 3-color Traffic Light assay for Candida species displayed >96% sensitivity and >95% specificity (Hall, Le Febre, Deml, Wohlfiel, & Wengenack, 2012) while their assay for detecting Enterococcus achieve >97% sensitivity and 100% specificity (Deck et al, 2014). Despite the promising results of the evaluation of the AdvanDx's assays, there are limitations: (1) the approach requires a positive culture, thus a key bottleneck-waiting for the culture to turn positive has not been eliminated; (2) the multiplexing abilities are limited to three distinct targets or groups, and given the range of targets that may induce sepsis, more than one assay is required to achieve sufficient coverage; and (3) the assay is an open system, prone to cross contamination, and although very simple, is also prone to human errors and inconsistencies.…”