Rapid Detection of Extended Spectrum β-Lactamase ( ESBL ) Producing Strains of Escherichia Coli in Urinary Tract Infections Patients in Benha University Hospital

Khater, E.; Sherif, Hammouda W

doi:10.12816/0004942

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…As regard distribution of different beta lactamases among E -coli; The ESBLs rate was the highest among the E -coli isolates and this agreed with other Egyptian studies conducted at Assiut, Banha, Alexandria, Sohag and Menoufia University hospitals. 24,25,26,27,28 Future studies may be carried out in larger sample size with application of genotypic methods which considered the gold to increase the sensitivity of the tests. Implementation of antibiotic policies stewardship and strict implementation of infection control measures to decrease antibiotic resistances.…”

Section: Discussionmentioning

confidence: 99%

“…We used the ESBL E-Test as a gold standard test. 7  ChromID™ ESBL agar (BioMérieux): Which is a selective chromogenic medium used according to manufacture instructions for the detection of Extended Spectrum ß-Lactamase producing enterobacteria , E. coli appear as pink to burgundy colonies or translucent colonies with a pink to burgundy centre.  Combined disk test (Oxoid): 6 The test evaluates the synergy between an oxyimino cephalosporin and clavulanic acid.…”

Section: Detection Of Esblsmentioning

confidence: 99%

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Different Phenotypic Methods for Detection of Beta Lactam Resistance in Escherichia coli

Rashed

Ahmed²,

Moawad³

2019

Egyptian Journal of Medical Microbiology

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Beta-lactam resistant Escherichia coli is a major cause of both nosocomial and community-acquired infections. It is essential to detect the beta lactam resistance for proper antibiotic therapy and to limit the spread the infection. Objectives: The study was designed to review the rates of extended-spectrum β-lactamases (ESBL), metallo beta lactamases (MBL) beta lactamase and AmpC production among E.coli isolates and to assess the best phenotypic method for detection of the resistance. Methodology: This study included 200 isolates obtained from patients admitted to different departments in Assiut university hospital .Screening and phenotypic confirmatory tests for resistance were done. Results: The Percentages of B -Lactamase enzymes in 5o isolates of E.coli were 6 (12%) isolates not resistant by screening with antibiotic sensitivity tests, 3 (6%) isolates were negative by the gold standard phenotypic tests, 12 (24%) were ESBL alone, 2 (4%) were ESBL and AmpC, 10 (20%) were AmpC alone, 8 (16%) were ESBL and Carbapenemases and 9 (18%) were carbapenemases alone.The combined disk test showed high sensitivity and specificity in detection of ESBL and MB, For AmpC detection; the disk approximation test showed higher sensitivity and specificity than boronic acid in detection of AmpC. Conclusion: The rate of Beta lactamases production in E.coli is seriously increased, the ESBL showed the highest percentage among E.coli isolates. The phenotypic confirmatory tests showed high sensitivity and specificity and proved to be reliable methods for detection of the beta lactamase resistance, genotypic tests are recommended to be a gold standard tests to increase the specificity of the phenotypic tests.

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Section: Discussionmentioning

confidence: 99%

Section: Detection Of Esblsmentioning

confidence: 99%

Different Phenotypic Methods for Detection of Beta Lactam Resistance in Escherichia coli

Rashed

Ahmed²,

Moawad³

2019

Egyptian Journal of Medical Microbiology

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Rapid Simultaneous Detection of AmpC and ESBLs among Enterobacteriaceae Using MastD68C Detection Set and Possible Therapeutic Options

Elsayed

Awad

Omar

et al. 2015

EJMM

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Background: Extended spectrum β-lactamases (ESBLs) and AmpC β-lactamases are enzymes produced by a variety of Gram-negative bacteria which confer an increased resistance to commonly used antibiotics and represent a substantial clinical threat. Several phenotypic tests have been recommended for screening and confirmation of ESBL-and AmpC-producing organisms. However, a comprehensive diagnostic algorithm integrating both screening and confirmation has not been established. Objectives: This study aimed to detect ESBL and/or AmpC production by using MastD68C ESBL and AmpC detection set as a single phenotypic method and to study its sensitivity and specificity comparing to other methods. Evaluate the effect of novel antibiotics namely tigecycline and doripenem, as well as the efficacy of old reviving antibiotics as colistin and temocillin against ESBL-and AmpC-producing Enterobacteriaecae. Methodology: Hundred Enterobacteriaceae isolates were screened for ESBL production using disc diffusion method and confirmed by combination disc diffusion test. Screening of AmpC production was done by cefoxitin disc test, disc approximation test and confirmation was done by AmpC disc test. Isolates screened positive for ESBL were investigated for their susceptibility to temocillin, tigecycline, colistin and doripenem by E-test. Results: Among the 100 Enterobacteriaceae isolates, 45 were screened positive for ESBL-production using the disc diffusion test and 36 were confirmed by the combination disc test. Nine isolates were screened for AmpCproduction using the cefoxitin disc test and 5 isolates were confirmed as AmpC producers by AmpC disc test. Using MAST D68C set, 35 isolates were ESBL producers, 2 were AmpC producers, one isolate was both ESBL and AmpC producer. All isolates were sensitive to tigecycline and doripenem. Forty-three isolates were sensitive to colistin, while, thirty-seven isolates were sensitive to temocillin. Conclusion: MAST D68C test appears to be a promising way to detect isolates producing ESBL and/or AmpC. Tigecycline, doripenem, temocillin and colistin revealed excellent activity against ESBL-and AmpC-producing Enterobacteriaceae.

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Fresh Garlic Extract has a Synergistic Effect with Antibiotics on ESBLs Producing E. coli Urinary Isolates

Abdelraheem

2019

JAMB

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Aim: Extended spectrum beta-lactamases (ESBLs)-producing bacteria often exhibit a multidrug- resistant phenotype limiting the therapeutic options available to the clinician. This study was conducted to investigate the antibacterial susceptibility pattern of ESBL producing E. coli isolates and evaluation of the antibacterial activity of Garlic extract against it. Study Design: The study was carried out using antibiotics powder and Garlic extract. Place and Duration of Study: At the microbiology lab, Faculty of Medicine, Minia University between January 2017 to August 2018. Methods: The study was carried on 55 ESBL producing E. coli isolates isolated from patients with urinary tract infections (UTI). Screening and confirmation for ESBL was done according to clinical and laboratory standard institute guidelines (CLSI) guidelines. The antibacterial sensitivity to a panel of antibiotics and Garlic was performed by tube dilution method. Results: The results of antibacterial susceptibility testing of ESBLs-producing E. coli isolates in our study showed a higher degree of resistance to the tested antibiotics. In the present study, Garlic showed an inhibitory effect on ESBLs- producing E. coli with concentrations ranging from 10-50 mg/ml and a mean of 30 mg/ml. Garlic extract produce a synergistic effect with all tested antibiotics against all tested ESBL producing E. coli isolates. A Significant decrease in ESBLs genes expression (SHV, TEM and CTX-m) was reported after treatment with Garlic extract (P value: ˂0.001). Conclusion: The combinations of antibiotics and Garlic may be more useful than individual agents, so we recommended that further researches should be undertaken to evaluate the combination of Garlic with antibiotics towards ESBL producing E. coli.

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Rapid Detection of Extended Spectrum β-Lactamase ( ESBL ) Producing Strains of Escherichia Coli in Urinary Tract Infections Patients in Benha University Hospital

Cited by 3 publications

References 14 publications

Different Phenotypic Methods for Detection of Beta Lactam Resistance in Escherichia coli

Different Phenotypic Methods for Detection of Beta Lactam Resistance in Escherichia coli

Rapid Simultaneous Detection of AmpC and ESBLs among Enterobacteriaceae Using MastD68C Detection Set and Possible Therapeutic Options

Fresh Garlic Extract has a Synergistic Effect with Antibiotics on ESBLs Producing E. coli Urinary Isolates

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