Rapid detection of extended-spectrum-β-lactamase-producing Enterobacteriaceae in blood cultures using the ESBL NDP test in Cotonou, Benin

Affolabi, Dissou; Sogbo, Fredéric; Laleye, Gracieux; Orekan, Jeanne; Massou, Faridath; Kehinde, Aderemi; Anagonou, S.

doi:10.1099/jmm.0.000509

Cited by 6 publications

(3 citation statements)

References 12 publications

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“…In Benin, several studies have reported the increasing evolution of the prevalence of broad-spectrum β-lactamase-producing enterobacteria in clinical infections [17][18][19]. But few studies have reported broad-spectrum β-lactamase-producing bacteria isolated simultaneously from external environment, kitchen environment, and food products.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Presence of Resistance Genes Detected from the Environment and Selected Food Products in Benin

Dougnon

Houssou²,

Anago

et al. 2021

Journal of Environmental and Public Health

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Gram-negative bacilli can spread from the environment and through food products. This study aimed to characterize ESBL production and virulence genes from multidrug-resistant Gram-negative bacilli isolated from specimen collected from the environment, kitchen, and food products. A total of 130 samples were collected at local markets in seven different communities in Benin (Abomey-Calavi, Ouidah, Bohicon, Abomey, Parakou, Djougou, and Grand-Popo). Samples were cultured on McConkey and ChromID™ ESBL agar plates. The isolates were identified by the API 20E gallery. An antibiotic susceptibility test was carried out, and the detection of ESBL production and virulence-associated genes was carried out by Polymerase Chain Reaction (PCR). The data collected was coded and analyzed using GraphPad prism 7 software and Excel. The software R was used to calculate the correlation coefficient between the results of the detection of ESBL+ on agar and by the effect of the double synergy. The results showed that sixty-three (63) bacterial strains were isolated from the 130 samples, of which the dominant species was Chryseomonas luteola (10/63). The kitchen samples were the most contaminated with 36.50%. More than 40% of the isolates were resistant to at least three different classes of antibiotics. Also, blaSHV gene was detected in 33.33% (21/63) of the isolates and in all isolates of Pseudomonas aeruginosa (5/5%). 11.11% (7/63) of isolates were virulent with dominance of the fimH gene, especially with Escherichia coli (83.33%). The kitchen samples showed a high prevalence of ESBL-producing strains with fimH gene. This raises the problem of non-compliance with hygiene rules in community cooking and food handling.

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Section: Introductionmentioning

confidence: 99%

Assessment of the Presence of Resistance Genes Detected from the Environment and Selected Food Products in Benin

Dougnon

Houssou²,

Anago

et al. 2021

Journal of Environmental and Public Health

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“…It is supposed that if the NDP test is implemented in routine practice, it might require additional costs, including personnel and laboratory costs, such as electricity, Eppendorf tubes, pipettes, and gloves. (20) There was a statistically significant reduction in LOT for patients who were given NDP test-guided antibiotic therapy in our randomized control trial. This might be due to the delay in the appropriate antibiotic intake in the group using standard culture-based therapy.…”

Section: Discussionmentioning

confidence: 61%

Evaluation of Nordmann–Dortet–Poirel Test as a Rapid and Direct Method of Detection of Extended-Spectrum β-Lactamases in Urinary Tract Infections

El-Attar

Baddour

Omar

et al. 2020

Journal of High Institute of Public Health

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Background & Objective(s): Antimicrobial resistance due to extended-spectrum β-lactamase (ESBL) production is a major public health issue. Its rapid detection is critical for early appropriate antibiotic use to prevent treatment failure, especially in cases of septicemia requiring appropriate empiric antibiotic therapy within the first few hours, thereby decreasing the mortality rate. Rapid detection is also important to spare the use of carbapenems, which, if used as a first-line drug in antibiotic policies, may lead to the emergence and spread of carbapenemases. We evaluated the Nordmann-Dortet-Poirel (NDP) test as a rapid method to detect ESBL producers directly from urine samples from patients with symptomatic urinary tract infections (SUTIs). Furthermore, we determined the clinical and economic outcomes of using NDP test results to guide antibiotic therapy. Methods: This cross-sectional study and double-blind, randomized control trial was conducted over 10 months. Urine samples were collected randomly from all patients with urinary tract infections admitted to the Internal Medicine Department at Alexandria University Hospital during the study period and assessed for eligibility. We enrolled 152 SUTI patients with gram-negative bacilli (≥10 5 cfu/ml), and the samples were tested for ESBLs using modified double-disk synergy testing (MDDST) and the NDP test. Patients were randomly divided into groups A or B, where culture-based therapy or NDP test-guided therapy was used first, respectively. All patients were observed for a clinical cure for at least 5 days. Results: The prevalence of ESBLs was 50% using MDDST. The overall sensitivity, specificity, positive predictive value, negative predictive value, and total accuracy for the ESBL NDP test performed directly on urine samples, using interpretable results, were 89.86%, 62.86%, 70.45%, 86.27%, and 76.26%, respectively. There was moderate agreement between the NDP test and MDDST and a statistically significant reduction in the length of antibiotic therapy (LOT) in the group using NDP test-guided therapy (p = 0.0002). Conclusion: The NDP test is a rapid and easy ESBL detection method that could be introduced in clinical practice. It is useful in guiding empiric therapy and reducing the LOT. A combination of ESBL NDP and Carba NP tests could be used in areas with a high prevalence of carbapenemases and ESBLs, but further studies are necessary to confirm efficacy.

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“…In the study by Duttaroy et al in India performed on 187 strains of E. coli and Klebsiella sp., 53 isolates (29.1%) were ESBL-producing (21). Also, among the ESBLproducing Enterobacteriaceae strains isolated from a hospital in France, a significant percentage of the isolated bacteria were E. coli (40.5%) (22). Therefore, it seems that the prevalence of ESBL-producing E. coli is relatively high in hospitals of Iran, which may be the cause of the high rate of resistance to antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Assessment of SHV, CTX-M, and IMP Genes in Beta-Lactam-Resistant Escherichia coli Isolated from Patients with Urinary Tract Infection

Abdolshahi

Aminian

Zinati

et al. 2019

Middle East J Rehabil Health Stud

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Objectives: Escherichia coli are resistant to β-lactam antibiotics due to the acquisition of plasmids that encode broad-spectrum β-lactamases. Extended-spectrum β-lactamases (ESBLs) have particular importance in antimicrobial therapy. The current study aimed at determining the frequency and role of SHV, CTX-M, and IMP genes in resistance to ceftriaxone, imipenem, and piperacillin antibiotics in E. coli isolated from patients with urinary tract infection (UTI). Methods: The study population included outpatients and inpatients of two genders in all ages with UTI. A total of 270 non-duplicate E. coli stains producing ESBL from Velayat Hospital, Damghan city, Semnan province, Iran were isolated using screening and doubledisc phenotypic test. All isolates were screened to detect SHV, CTX-M, and IMP genes using polymerase chain reaction (PCR). Results: In total, 120 (44.4%) samples were positive for ESBLs including 30% CTX-M, 27.5% SHV, and 0% IMP genes. The obtained results showed that the responses of male and female patients to the examined antibiotic were different. The distribution of underlying diseases (diabetes, kidney stones, obstructive disease, congenital anomalies, and hospitalization history) was different in patients with UTI. It was observed that after hospitalization history, diabetes had the highest frequency in female (31.08%) and male (27.59%) patients with UTI. Conclusions: However, bacterial strains producing ESBLs such as E. coli should be considered as a major threat to public health due to the risk of resistant to broad-spectrum cephalosporins. Therefore, to prevent resistance to antibiotics, indiscriminate administration of such agents should be avoided.

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Rapid detection of extended-spectrum-β-lactamase-producing Enterobacteriaceae in blood cultures using the ESBL NDP test in Cotonou, Benin

Abstract: The ESBL NDP test is rapid, relatively affordable and performed well in our setting.

Cited by 6 publications

References 12 publications

Assessment of the Presence of Resistance Genes Detected from the Environment and Selected Food Products in Benin

Assessment of the Presence of Resistance Genes Detected from the Environment and Selected Food Products in Benin

Evaluation of Nordmann–Dortet–Poirel Test as a Rapid and Direct Method of Detection of Extended-Spectrum β-Lactamases in Urinary Tract Infections

Assessment of SHV, CTX-M, and IMP Genes in Beta-Lactam-Resistant Escherichia coli Isolated from Patients with Urinary Tract Infection

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