Rapid detection of genetic variability in chrysanthemum (Dendranthema grandiflora Tzvelev) using random primers

Wolff, Kirsten; Rijn, J. Peters-van

doi:10.1038/hdy.1993.147

Cited by 101 publications

(52 citation statements)

References 37 publications

(34 reference statements)

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“…Alternatively, diagnostic marker bands of parents may have been lost from S. cambrensis due to rapid genomic change, which is believed to occur in new allopolyploids more commonly than previously assumed (Soltis & Soltis 1999, 2000. A third possibility is that band absence is due to the effect of ploidy upon competition processes in RAPD-PCR reactions (Wolff & Peters-Van Rijn 1993;Weising et al 1995). Because S. cambrensis contains a larger genome than S. squalidus, it is expected to possess more sites of 100% sequence similarity to a particular primer.…”

Section: Genomic Composition Of Hybrid Derivatives Of Senecio Squalidmentioning

confidence: 99%

Plant introductions, hybridization and gene flow

Abbott

James

Milne

et al. 2003

Phil. Trans. R. Soc. Lond. B

140

121

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Many regional floras contain a high proportion of recently introduced plant species. Occasionally, hybridization between an introduced species and another species (introduced or native) can result in interspecific gene flow. This may occur even in instances where the F 1 hybrid shows very high sterility, but occasionally produces a few viable gametes. We provide examples of gene flow occurring between some rhododendrons recently introduced to the British flora, and between an introduced and native Senecio species. Neutral molecular markers have normally been employed to obtain evidence of interspecific gene flow, but the challenge now is to isolate and characterize functional introgressed genes and to determine how they affect the fitness of introgressants and whether they improve adaptation to novel habitats allowing introgressants to expand the range of a species. We outline a candidate gene approach for isolating and characterizing an allele of the RAY gene in Senecio vulgaris, which is believed to have introgressed from S. squalidus, and which causes the production of ray florets in flower heads. We discuss the effects of this introgressed allele on individual fitness, including those that originate directly from the production of ray florets plus those that may arise from pleiotropy and/or linkage.

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Section: Genomic Composition Of Hybrid Derivatives Of Senecio Squalidmentioning

confidence: 99%

Plant introductions, hybridization and gene flow

Abbott

James

Milne

et al. 2003

Phil. Trans. R. Soc. Lond. B

140

121

View full text Add to dashboard Cite

show abstract

“…These techniques have been applied to wild species to answer specific questions, mainly regarding the crossing system (Hayward and Hamilton, 1997). Some studies based on microsatellites have been employed in the study of natural populations (Collevatti et al, 2001;Zucchi et al, 2003) but most of the studies are performed with RAPD markers in plants (Chalmers et al, 1992;Wolf andRijn, 1993, Bucci andMenozzi, 1995;Yeh et al, 1995;Maguire and Sedgley 1997;Gillies et al, 1997, Palácios andGonzález-Candelas, 1997;Gallois et al, 1998, Cattan-Toupance et al, 1998, Skabo et al, 1998, and in other organisms (Peever andMilgroom, 1994, Lou et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Genetic structure of natural Eugenia dysenterica DC (Myrtaceae) populations in northeastern Goiás, Brazil, accessed by morphological traits and RAPD markers

Trindade

Chaves

2005

Genet. Mol. Biol.

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The Brazilian Cerrado (Savannah) is a biome with great biodiversity. Many of the species found in this biome have unlimited economic potential and are used by the local population for various purposes. With the opening of the agricultural frontiers in this region, much of the native flora has been replaced by extensive areas of monocultures. Thirteen populations of the Eugenia dysenterica DC species were sampled in the northeast of the state of Goiás, Brazil, to analyze their genetic structure. Morphological traits were measured for all the progeny obtained and some of them were analyzed by using RAPD markers. The natural populations of the species showed high levels of variability for the morphological traits. The phenotypic, genotypic and genetic variabilities as structured in these populations were highly significant among populations, although most of the variability was detected within populations. The phenotypic and genetic variabilities were structured spatially as shown by the positive and highly significant correlation coefficients between the phenotypic and genetic distance matrixes and the geographic distance matrix. The ideal conservation strategy for the species in the region requires sampling of a high number of individuals of each population and also a significant number of populations to ensure suitable effective population sizes.

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“…Biochemical and molecular markers have been used to enhance understanding of plant population genetic structure (Soltis et al, 1992). Among several efficient methods for revealing genetic variability within and among plant populations, some of the most widely applied are isozyme electrophoresis (Hamrick and Allard, 1972;Hamrick and Godt, 1990), random amplified DNA polymorphism -RAPDs - (Wolff and Peters-van Rijn, 1993;Wachira et al, 1995;Brummer et al, 1995;Swoboda and Bhalla, 1997;Palacios and Gonzalez-Candelas, 1997), and restriction fragment length polymorphisms -RFLPs - (Keim et al, 1989;Hong et al, 1993;Yanesita et al, 1997). In the genus Prosopis, previous isoenzymatic studies in some Algarobia Section species have shown low differentiation among species and populations coupled with high variability within populations (Saidman, 1985(Saidman, , 1986(Saidman, , 1990(Saidman, , 1993Saidman and Vilardi, 1987;Verga, 1995).…”

Section: Introductionmentioning

confidence: 99%

Isozyme and RAPD studies in Prosopis glandulosa and P. Velutina (Leguminosae, Mimosoideae)

2000

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Allozyme and random amplified polymorphic DNA (RAPD) techniques have been compared for their usefulness for genetic and taxonomic studies in Prosopis glandulosa and P. velutina populations. Isozymes and RAPDs yielded similarly high estimates of genetic variability. Genetic structure and differentiation were analyzed through non-hierarchical Wright's F DT . For all populations considered, both markers produced low gene flow (Nm < 1) estimates. When only P. glandulosa populations were analyzed, isozyme data yielded higher gene flow estimates (Nm > 1), in agreement with that expected for conspecific populations. However, in RAPD data the expected reduction in F DT and the increase in Nm were not observed. Correlation between F DT and geographical distance matrices (Mantel test) for all populations was significant (P = 0.02) when based on isozymes, but not so (P = 0.33) when based on RAPDs. No significant associations among genetic and geographical or climatic variables were observed. Two isoenzyme systems (GOT and PRX) enabled us to distinguish between P. glandulosa and P. velutina, but no diagnostic band for recognition of populations or species studied here were detected by RAPD. However, RAPD markers showed higher values for genetic differentiation among conspecific populations of P. glandulosa and a lower coefficient of variation than those obtained from isozymes.

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Rapid detection of genetic variability in chrysanthemum (Dendranthema grandiflora Tzvelev) using random primers

Cited by 101 publications

References 37 publications

Plant introductions, hybridization and gene flow

Plant introductions, hybridization and gene flow

Genetic structure of natural Eugenia dysenterica DC (Myrtaceae) populations in northeastern Goiás, Brazil, accessed by morphological traits and RAPD markers

Isozyme and RAPD studies in Prosopis glandulosa and P. Velutina (Leguminosae, Mimosoideae)

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