Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction

Abstract

By using primers based on the sequence of a species-specific antigen of Helicobacterpylori (P. O'Toole, S. M.

No Correlation of babA2 with vacA and cagA Genotypes of Helicobacter pylori and Grading of Gastritis from Peptic Ulcer Disease Patients in Brazil

“…The final extension at 72 °C was for 7 minutes. One set of primers (P1 and P2) [29] that amplifies a 26-kDa antigen gene (Accession: M55507) present in all strains of H. pylori was also used according to the following conditions: initial denaturation at 94 °C for 5 minutes followed by 40 cycles of denaturation at 93 °C for 1 minute, annealing at 57 °C for 2 minutes, and extension at 70 °C for 2 minutes.…”

Helicobacter pylori Recurrence in Patients with Duodenal Ulcer: Clinical, Endoscopic, Histologic, and Genotypic Aspects. A 10‐Year Brazilian Series

“…The final extension at 72 ° C was performed for 7 minutes. One set of primers (P1 and P2) [24] that amplifies a 26 kDa antigen gene present in all strains of H. pylori was also used according to the following conditions: initial denaturation at 94 ° C for 5 minutes followed by 40 cycles of denaturation at 93 ° C for 1 minute, annealing at 57 ° C for 2 minutes and extension at 70 ° C for 2 minutes.…”

Combination ofHelicobacter pylori-iceA2and proinflammatory interleukin-1 polymorphisms is associated with the severity of histological changes in Venezuelan chronic gastritis patients

“…Helicobacter pylori infection was confirmed by PCR reactions using two sets of oligonucleotides: H3-H4, which amplifies a 298-bp fragment corresponding to a gene encoding a 26-kDa antigenic protein (Hammar et al, 1992), and Hp1-Hpx2, which amplifies a 150-bp fragment corresponding to 16S rRNA gene (Scholte et al, 1997). Samples were considered H. pylori-positive when PCR product was obtained in at least one reaction.…”