Rapid Detection of <i>Escherichia coli</i> by Using Antibody‐Conjugated Silver Nanoshells

Kalele, Suchita; Kundu, Anita A.; Gosavi, Suresh; Deobagkar, Dileep N.; Deobagkar, Deepti D.; Kulkarni, Sulabha K.

doi:10.1002/smll.200500286

Cited by 103 publications

(59 citation statements)

References 23 publications

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“…Nanoshells are another type of metal nanostructures with interesting optical properties useful for various applications [59][60][61][62][63]. These structures, which are typically made of spherical silica cores surrounded by a layer of noble metal aggregates, have variation in size, shape and surface morphology due to the random nature of aggregation [64,65].…”

Section: Metal Nanostructuresmentioning

confidence: 99%

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

2016

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By electrodeposition and galvanic replacement reaction, we developed a facile, time-saving, cost-effective, and environmentally friendly, two-step synthesis route to obtain a controllable cobalt oxide/Au hierarchically nanostructured electrode for glucose sensing. The nanomaterials were characterized by transmission electron microscopy, scanning electron microscopy, Raman spectroscopy, energy-dispersive spectrometry, and X-ray photoelectron spectroscopy, meanwhile, the sensing performance was investigated by cyclic voltammetry and amperometric response. The results revealed that this novel electrode exhibited excellent electrocatalytic performance toward glucose oxidation, with a wide double-linear range from 0.2 μM to 20 mM and a low detection limit of 0.1 μM based on a signal-to-noise ratio of 3, which was mainly attributed to the ability of loading a small amount of Au with good electron conductivity on the surface of cobalt oxide nanosheets with large active surface area and synergistic electrocatalytic activity of Au and cobalt oxide toward glucose electrooxidation. This facile, sensitive, and selective glucose sensor is also proven to be suitable for the detection of glucose in human serum.

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Section: Metal Nanostructuresmentioning

confidence: 99%

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

2016

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“…[3][4][5][6][7] Many new types of nanomaterials are being released in significant amounts with wide variety of applications in areas such as medicine, diagnostics, drug delivery, 8 and biosensors. 9,10 The increasing applications of NPs in various fields have led to an increase in human exposure which in turn may lead to toxicological effects and unexpected health and environmental hazards. 11,12 Among the intentionally engineered NPs, metal oxide NPs are the most widely produced and used nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

Epigenetic modulation upon exposure of lung fibroblasts to TiO<sub>2</sub> and ZnO nanoparticles: alterations in DNA methylation

Patil

Gade

Deobagkar

2016

IJN

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Titanium dioxide (TiO 2 ) and zinc oxide (ZnO) nanoparticles (NPs) are promising candidates for numerous applications in consumer products. This will lead to increased human exposure, thus posing a threat to human health. Both these types of NPs have been studied for their cell toxicity, immunotoxicity, and genotoxicity. However, effects of these NPs on epigenetic modulations have not been studied. Epigenetics is an important link in the genotype and phenotype modulation and misregulation can often lead to lifestyle diseases. In this study, we have evaluated the DNA methylation-based epigenetic changes upon exposure to various concentrations of NPs. The investigation was designed to evaluate global DNA methylation, estimating the corresponding methyltransferase activity and expression of Dnmt gene using lung fibroblast (MRC5) cell line as lungs are the primary route of entry and target of occupational exposure to TiO 2 and ZnO NPs. Enzyme-linked immunosorbent assay-based immunochemical assay revealed dose-related decrease in global DNA methylation and DNA methyltransferase activity. We also found direct correlation between the concentration of NPs, global methylation levels, and expression levels of Dnmt1, 3A, and 3B genes upon exposure. This is the first study to investigate effect of exposure to TiO 2 and ZnO on DNA methylation levels in MRC5 cells. Epigenetic processes are known to play an important role in reprogramming and adaptation ability of an organism and can have long-term consequences. We suggest that changes in DNA methylation can serve as good biomarkers for early exposure to NPs since they occur at concentrations well below the sublethal levels. Our results demonstrate a clear epigenetic alteration in response to metal oxide NPs and that this effect was dose-dependent.

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“…1 AgNPs have been exploited in designing silica-silver core-shell nanoparticles and utilized for rapid detection of microbes, proteins, and antibodies. 2,3 AgNPs have also been used in bone cements in artificial joint replacements, since their presence drastically reduced the wear and tear of the polymer. 4,5 Although AgNPs have promising role in biomedical applications, human toxicity and environmental hazards posed by them cannot be neglected.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity

Kulkarni¹,

Shaiwale²,

Deobagkar³

et al. 2015

IJN

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There has been rapid progress in exploring microorganisms for green synthesis of nanoparticles since microbes show extraordinary diversity in terms of species richness and niche localization. Microorganisms are easy to culture using relatively inexpensive and simple nutrients under varied conditions of temperature, pressure, pH, etc. In this work, Deinococcus radiodurans that possesses the ability to withstand extremely high radiation and desiccation stress has been employed for the synthesis of silver nanoparticles (AgNPs). D. radiodurans was able to accumulate AgNPs in medium under various conditions, and process optimization was carried out with respect to time, temperature, pH, and concentration of silver salt. AgNPs were characterized using UV/vis spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The microbially synthesized AgNPs exhibited good antimicrobial activity against both Gram-negative and Gram-positive organisms and anti-biofouling activity. Their ability to inhibit growth and proliferation of cancer cell line was also examined, and it could be seen that AgNPs synthesized using D. radiodurans exhibited excellent anticancer activity.

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Rapid Detection of Escherichia coli by Using Antibody‐Conjugated Silver Nanoshells

Cited by 103 publications

References 23 publications

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

Epigenetic modulation upon exposure of lung fibroblasts to TiO<sub>2</sub> and ZnO nanoparticles: alterations in DNA methylation

Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity

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