2009
DOI: 10.1002/elps.200900136
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Rapid detection of Mycoplasma pneumonia in a microfluidic device using immunoagglutination assay and static light scattering

Abstract: We present real-time, rapid detection of Mycoplasma pneumonia in PBS inside a Y-channel PDMS microfluidic device via optical fiber monitoring of latex immunoagglutination. The latex immunoagglutination assay was performed with serially diluted M. pneumonia solutions using highly carboxylated polystyrene particles of 390 and 500 nm diameter conjugated with monoclonal anti-M. pneumonia. Proximity optical fibers were located around the viewing cell of the device, which were used to measure the increase in 45 degr… Show more

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Cited by 10 publications
(10 citation statements)
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“…Conventional assay methods used for diagnosis of the pulmonary disease primarily include spirometry, cell migration assay, 9 acid fast smear using sputum, 12 gene expression analysis, 15 cell culture assays, [16][17] interferon gamma release assay. 18 In addition, for understanding the mechanism of pulmonary diseases, researchers have conducted their studies conventionally in human patients, 9 animal models, 4,14 and using in vitro lung tissue slices.…”
Section: Conventional Study Models and Diagnostic Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…Conventional assay methods used for diagnosis of the pulmonary disease primarily include spirometry, cell migration assay, 9 acid fast smear using sputum, 12 gene expression analysis, 15 cell culture assays, [16][17] interferon gamma release assay. 18 In addition, for understanding the mechanism of pulmonary diseases, researchers have conducted their studies conventionally in human patients, 9 animal models, 4,14 and using in vitro lung tissue slices.…”
Section: Conventional Study Models and Diagnostic Methodsmentioning
confidence: 99%
“…12 Gene expression analysis Laborious, samples are subjected to considerable ex-vivo perturbation resulting in documented phenotypic and functional changes. 15 Cell culture assay Unable to mimic the in vivo conditions, 16,17 contamination is a big challenge. 17 Interferon gamma release assay Unable to count the number of CD4 cells secreting the cytokine, that makes them less accurate.…”
Section: Conventional Diagnostic Methods Shortcomingsmentioning
confidence: 99%
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“…Conventional assay methods used for diagnosis of the pulmonary disease primarily include spirometry, cell migration assay [9], acid fast smear using sputum [12], gene expression analysis [15], cell culture assays [16][17], interferon gamma release assay [18]. In addition, for understanding the mechanism of pulmonary diseases, researchers have conducted their studies conventionally in human patients [9], animal models [4], [14] and using in vitro lung tissue slices [16].…”
Section: Conventional Study Models and Diagnostic Methodsmentioning
confidence: 99%
“…Although there have been a number of optical approaches with microfluidic devices including surface enhanced Raman scattering, interferometry, and particle beam emission spectroscopy [12][13][14], little effort has been made Keesung Kim 1,2 Man-Bock Gu toward the development of light scattering detection inside a microfluidic device [15,16]. In this sense, the so-called ''light scattering particle immunoagglutination assay'' (LSPIA) could be a new, alternative method for rapid and high-sensitivity detection of proteins, viruses and bacteria [17][18][19].…”
Section: Introductionmentioning
confidence: 99%