Rapid Detection of Pseudomonas in Seafoods Using Protease Indicator

Abstract

The effects of Pseudomonas and the proteases it synthesizes in seafoods rendering them unfit for consumption are not fully recognized. A sandwich enzymelinked immunosorbent assay (ELISA) was developed wherein protease produced by Pseudomonas isolated from shrimp was used as antigen, and anti-protease IgG conjugated with alkaline phosphatase was the second antibody. Purified protease and seafood samples, naturally contaminated or artificially inoculated with Pseudomonas, were positive by ELISA. The conventional culture method took 3 days to complete, but ELISA detected Pseudomonas within 24h. The rapidity, simplicity and efficacy of this test make it useful for implementation of HACCP systems.

Quality Indicators of Cultured Newfoundland Blue Mussels (Mytilus edulis) during Storage on Ice: Microbial Growth, pH, Lipid Oxidation, Chemical Composition Characteristics, and Microbial Fatty Acid Contents

“…Various statistical parameters were used to examine the strength of the examined linear relationships including the correlation coefficient ( r ) and level of significance ( p ) values. Relationships with r < 0.5 and p > 0.05 were considered to be poorly correlated, relationships with r = 0.500−0.700 and p < 0.05 were considered moderately correlated, and relationships with r > 0.700 and p < 0.05 were strongly correlated ( − ).…”

Synchronous front-face fluorescence spectroscopy as a promising tool for the rapid determination of spoilage bacteria on chicken breast fillet

“…In recent years some new techniques were introduced to replace the culture dependent techniques. Several methods have been used since last two decades including some enumeration techniques based on microscopy (Pyle, Broadaway, & McFeters, 1999), ATP bioluminescence (Champiat, Matas, Monfort, & Fraass, 2001) and measurement of electrical phenomenon Food Research International 44 (2011) 471-480 (Betts, 1999) or some detection methods including immunological (Jabbar & Joishy, 1999) and molecular-based techniques (ELISA, PCR, etc.) (Mullis & Faloona, 1987).…”

Efecto de enzimas proteolíticas endógenas y una bacteriana en la formación de emulsiones modelo a partir de músculo estriado de cazón (Rhizopriondon terraenovae) y cerdo

“…Pseudomonas son un grupo dominante de contaminantes activos de sustratos musculares de origen terrestre o marino (Jabbar y Joishy, 1999).…”