Rapid detection of <i>Salmonella</i> using an aptamer-functionalized PDA liposome sensor with naked-eye colorimetric sensing

Lee, Goeun; Kim, Byeongsung; Jang, Inseung; Il Kim, Moon; Shin, Seunghan; Kwon, Kiok

doi:10.1039/d3ma00840a

Aptamer and DNAzyme Based Colorimetric Biosensors for Pathogen Detection

Liu,

Li,

Salena

et al. 2024

Angewandte Chemie

0

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The detection of pathogens is critical for preventing and controlling health hazards across clinical, environmental, and food safety sectors. Functional nucleic acids (FNAs), such as aptamers and DNAzymes, have emerged as versatile molecular tools for pathogen detection due to their high specificity and affinity. This review focuses on the in vitro selection of FNAs for pathogens, with emphasis on the selection of aptamers for specific biomarkers and intact pathogens, including bacteria and viruses. Additionally, the selection of DNAzymes for bacterial detection is discussed. The integration of these FNAs into colorimetric biosensors has enabled the development of simple, cost‐effective diagnostic platforms. Both non‐catalytic and catalytic colorimetric biosensors are explored, including those based on gold nanoparticles (AuNPs), polydiacetylenes (PDAs), protein enzymes, G‐quadruplexes, and nanozymes. These biosensors offer visible detection through color changes, making them ideal for point‐of‐care diagnostics. The review concludes by highlighting current challenges and future perspectives for advancing FNA‐based colorimetric biosensing technologies for pathogen detection.

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Aptamer and DNAzyme Based Colorimetric Biosensors for Pathogen Detection

Liu,

Li,

Salena

et al. 2024

Angew Chem Int Ed

0

View full text Add to dashboard Cite

The detection of pathogens is critical for preventing and controlling health hazards across clinical, environmental, and food safety sectors. Functional nucleic acids (FNAs), such as aptamers and DNAzymes, have emerged as versatile molecular tools for pathogen detection due to their high specificity and affinity. This review focuses on the in vitro selection of FNAs for pathogens, with emphasis on the selection of aptamers for specific biomarkers and intact pathogens, including bacteria and viruses. Additionally, the selection of DNAzymes for bacterial detection is discussed. The integration of these FNAs into colorimetric biosensors has enabled the development of simple, cost‐effective diagnostic platforms. Both non‐catalytic and catalytic colorimetric biosensors are explored, including those based on gold nanoparticles (AuNPs), polydiacetylenes (PDAs), protein enzymes, G‐quadruplexes, and nanozymes. These biosensors offer visible detection through color changes, making them ideal for point‐of‐care diagnostics. The review concludes by highlighting current challenges and future perspectives for advancing FNA‐based colorimetric biosensing technologies for pathogen detection.

show abstract