Rapid Detection of
            <i>Staphylococcus aureus</i>
            and Methicillin-Resistant
            <i>S. aureus</i>
            (MRSA) in Wound Specimens and Blood Cultures: Multicenter Preclinical Evaluation of the Cepheid Xpert MRSA/SA Skin and Soft Tissue and Blood Culture Assays

Wolk, Donna M.; Struelens, Marc; Pancholi, Preeti; Davis, Thomas P.; Della‐Latta, Phyllis; Fuller, D.; Picton, E.; Dickenson, Roberta; Denis, Olivier; Johnson, David A.; Chapin, Kimberle

doi:10.1128/jcm.01884-08

Cited by 205 publications

(149 citation statements)

References 14 publications

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“…Our data, with 100 % sensitivity for the detection of Staphylococcus aureus and mecA, are similar to contemporary work with the various RT-PCR assays employed in previously published studies detecting meticillin-sensitive Staphylococcus aureus (MSSA) and MRSA, with sensitivities of 100 % and between 97.9 and 100 %, respectively (Paule et al, 2005;Parta et al, 2009;Wolk et al, 2009;Jukes et al, 2010). The MT-PCR assay described here has the added advantage of also being a rapid and accurate diagnostic tool for the detection of Streptococcus pneumoniae and Enterococcus species, including VRE.…”

Section: Discussionsupporting

confidence: 76%

Rapid identification of Gram-positive pathogens and their resistance genes from positive blood culture broth using a multiplex tandem RT-PCR assay

Hazelton

Thomas

Unver

et al. 2013

Journal of Medical Microbiology

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The early initiation of targeted antibiotic therapy in patients with bacteraemia and septic shock impacts favourably on outcomes. Rapid methods are therefore increasingly employed for bacterial identification directly from positive blood culture bottles, but with variable success. We evaluated the performance of the Gram Positive 12 multiplex tandem PCR (MT-PCR) assay (AusDiagnostics; catalogue no. 6202, version 07) containing targets for the identification of staphylococci including Staphylococcus aureus, streptococci including Streptococcus pneumoniae, enterococci including Enterococcus faecalis and Enterococcus faecium and their common antibiotic resistance genes (mecA, vanA, vanB). A total of 673 aerobic and anaerobic blood culture broths demonstrating Gram-positive cocci on microscopy were analysed in parallel with traditional phenotypic methods. Amplification of the internal control was inhibited in 79/673 (11.7 %) samples; however, MT-PCR identification was in concordance with phenotypic identification to the genus level in 96.6 % (537/556) of the remaining monomicrobial specimens and to the species level, where applicable, in 100 % (172/172) of samples. MT-PCR identification for 94.7 % (36/38) of polymicrobial samples matched traditional phenotypic identification. Meticillin and vancomycin susceptibility results determined by MT-PCR in blood culture broths demonstrated complete agreement with those determined by phenotypic methods in all 143 Staphylococcus aureus isolates and eight E. faecium isolates, respectively. Gram-positive pathogens and their key antibiotic resistance markers were reliably identified with the MT-PCR assay within 3 h of a positive blood culture result.

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Section: Discussionsupporting

confidence: 76%

Rapid identification of Gram-positive pathogens and their resistance genes from positive blood culture broth using a multiplex tandem RT-PCR assay

Hazelton

Thomas

Unver

et al. 2013

Journal of Medical Microbiology

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“…No nonstaphylococcal Gram-positive or Gram-negative bacterial species were detected by either the mecA or SA primerprobe sets with this assay. Previous studies have employed methicillin-resistant CoNS (MRCoNS) and methicillin-sensitive CoNS (MSCoNS) strains to evaluate false-positive detection with inhouse real-time PCR targeting the orfX-SCCmec junction (23), as well as the GeneXpert and GeneOhm assays (24,25,26). In our case, we lost no sensitivity or specificity for our assay when MRSA cultures were spiked with either MSSA or MSCoNS.…”

Section: Discussionmentioning

confidence: 73%

“…Compared to direct agar culture methods, Wolk and colleagues (24) reported 15.7% PCRpositive culture-negative specimens. There are several explanations for this discrepancy, such as nonviable organisms that are present at cultivation detected by PCR, the presence of SCCmec variants, or low densities of organisms (24,25). Both the GeneXpert MRSA and GeneOhm assays were found to have false positives due to mecA deletion strains (28,29).…”

Section: Discussionmentioning

confidence: 97%

Detection of Methicillin-Resistant Staphylococcus aureus by a Duplex Droplet Digital PCR Assay

et al. 2013

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bHealth care-associated infections with methicillin-resistant Staphylococcus aureus (MRSA) contribute to significant hospitalization costs. We report here a droplet digital PCR (ddPCR) assay, which is a next-generation emulsion-based endpoint PCR assay for high-precision MRSA analysis. Reference cultures of MRSA, methicillin-susceptible S. aureus (MSSA), and confounders were included as controls. Copan swabs were used to sample cultures and collect specimens for analysis from patients at a large teaching hospital. Swab extraction and cell lysis were accomplished using magnetic-driven agitation of silica beads. Quantitative PCR (qPCR) (Roche Light Cycler 480) and ddPCR (Bio-Rad QX100 droplet digital PCR system) assays were used to detect genes for the staphylococcal protein SA0140 (SA) and the methicillin resistance (mecA) gene employing standard TaqMan

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“…The commonly used commercial kits based on molecular methods include the BD GeneOhmTM MRSA ACP assay, the GeneXpert® MRSA assay, the Hyplex Staphylo Resist test system, and the BD GeneOhmTM StaphSR assay (5,7,20). However, these molecular methods cannot be used in most clinical microbiology laboratories in Turkey due to their high costs and lack of required technical equipment.…”

Section: Discussionmentioning

confidence: 99%

Same-day Detection of Methicillin Resistance in Staphylococcus aureus Isolates by StaResMet® Kit

Sezgin

Vural

Kiraz

et al. 2017

Jundishapur J Microbiol

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Background: Staphylococcus aureus is an important cause of hospital-acquired infections. The most important issue with S. aureus is that the isolates are getting increasingly methicillin-resistant. Rapid differentiation between methicillin-resistant and methicillinsusceptible S. aureus species is necessary to optimize treatment and minimize costs. Objectives: The current study aimed at evaluating the StaResMet® kit for rapid detection of methicillin resistance in S. aureus isolates. Methods: A total of 217 methicillin-resistant S. aureus (MRSA) and 252 methicillin-susceptible S. aureus (MSSA) isolates were tested using the StaResMet® kit. The test was performed according to the manufacturer's instructions. Results: The kit identified the MRSA isolates with 100% accuracy, and found that the minimum inhibitory concentrations (MICs)

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Rapid Detection of Staphylococcus aureus and Methicillin-Resistant S. aureus (MRSA) in Wound Specimens and Blood Cultures: Multicenter Preclinical Evaluation of the Cepheid Xpert MRSA/SA Skin and Soft Tissue and Blood Culture Assays

Cited by 205 publications

References 14 publications

Rapid identification of Gram-positive pathogens and their resistance genes from positive blood culture broth using a multiplex tandem RT-PCR assay

Rapid identification of Gram-positive pathogens and their resistance genes from positive blood culture broth using a multiplex tandem RT-PCR assay

Detection of Methicillin-Resistant Staphylococcus aureus by a Duplex Droplet Digital PCR Assay

Same-day Detection of Methicillin Resistance in Staphylococcus aureus Isolates by StaResMet® Kit

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