Rapid Detection of Microbial Contamination in Triclosan and High Fluoride Dentifrices Using an Atp Bioluminescence Assay

Abstract

The Celsis ATP Bioluminescence method was optimized and validated to detect the presence of microbial contamination in High Fluoride and Triclosan dentifrice formulations. Several enrichment broths were evaluated by using a 24–27 h incubation period. The ATP concentrations of the enrichment broths were found to a range from 0.012 to 0.040 nM. None of the tested enrichment broths were found to exhibit any sample inhibition/enhancement effects on the ATP Bioluminescence reaction. Dentifrice suspensions were inoculated with bacteria, yeast, and mold. All test microorganisms (ca. 1–15 CFU/g) were detected within a 24–27 h incubation period by using TAT Broth Base enrichment broths containing different concentrations of the following ingredients: Tween 20, Neopeptone, Dextrose, Triton X‐100, Thiosulfate, Sodium Dibasic Phosphate, and Glycine. Negative ATP response after 24–27 h of incubation at 35C indicates the absence of contamination from these products.

Submersible microbial fuel cell sensor for monitoring microbial activity and BOD in groundwater: Focusing on impact of anodic biofilm on sensor applicability

“…6). The current density increased gradually with operation time and reached its highest level (0.99 ± 0.01 mA/m 2 ) after 3 h. According to the linear relationship established in Figure 4B, the microbial activity in the real groundwater was estimated to a microbial ATP level of 0.064 ± 0.006 nmol/L, which was close to 0.081 ± 0.005 nmol/L of ATP measured by standard method (Ignar et al, 1998). After switching to the biofilm‐colonized anode, a stable current density (222 ± 1 mA/m 2 ) was produced immediately (≤0.08 h), resulting in a total response time <3.1 h for both microbial activity and BOD monitoring (Fig.…”

“…The current generations with other samples were showed the same trends, and the results were summarized in Table I. The deviations between the ATP concentration measured by the sensor and standard method (Ignar et al, 1998) were ranging from 15% to 22%, while the deviations between the BOD measured by the sensor and APHA method were ranging from 6% to 16% for these four real samples. Repeated BOD measurements by the sensor showed standard deviations from ±2% to ±6%, which are lower than the deviation from conventional BOD 5‐day tests, where deviation of ±15% is allowed (Kumlanghan et al, 2007).…”

“…92521) dissolved in Lumin (PM) buffer (Celsis No. 92588) and diluted in sterile tap water (Ignar et al, 1998).…”

Detection of indicator pathogens from pharmaceutical finished products and raw materials using multiplex PCR and comparison with conventional microbiological methods

“…Standard microbiological analysis of pharmaceutical samples requires 5-7 days to be completed [4,28,39]. However, rapid methods have given results within 24-30 h [12,13]. Nevertheless, companies have relied on these methods for quality evaluation of raw materials and Wnished products [4,5].…”